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[Effect of electroacupuncture stimulation of "Fengfu" (GV 16) and "Taichong" (LR 3) on expression of COX-2 and tyrosine hydroxylase in Substantia Nigra in rats with Parkinson's disease].

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) therapy on the expression of tyrosine hydroxylase (TH) and cyclooxygenase-2 (COX-2) proteins of Substantia Nigra cells(SNc) in the rotenone-induced Parkinson's disease(PD) rats, so as to explore the mechanism of EA underlying improvement of PD.

METHODS

Forty rats were randomly divided into normal, sham-operation (sham), model and EA groups (n = 10/group). The PD model was established by successive subcutaneous injection of rotenone (highly selective lesions of nigrostriatal dopaminergic neurons) for 28 days. EA (2 Hz, 1 mA) was applied to bilateral "Fengfu" (GV 16) and "Taichong" (LR 3) for 20 min, once daily for 14 days. The expression levels of TH and COX-2 proteins in the Substantia Nigra of midbrain were detected with Western blotting.

RESULTS

Compared with the normal group, the expression level of TH protein in the model group was significantly decreased (P < 0.01), and that of COX-2 protein in the model group was significantly increased (P < 0.01). After the EA treatment, the expression level of TH in the EA group was obviously upregulated (P < 0.01), and that of COX-2 protein in the EA group was considerably down-regulated (P < 0.01). No significant differences were found between the normal and sham groups in the expression levels of TH and COX-2 proteins (P > 0.05).

CONCLUSION

EA therapy can decrease inflammation mediator COX-2 protein expression and upregulate TH protein expression in the Substantia Nigra of midbrain in PD rats, which may contribute to its effect in relieving PD in clinic.

Authors+Show Affiliations

,

The Third Clinical College of Zhejiang Chinese Medical University, Hangzhou 310005, China.

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Source

MeSH

Acupuncture Points
Animals
Cyclooxygenase 2
Electroacupuncture
Gene Expression
Humans
Male
Parkinson Disease
Rats
Rats, Sprague-Dawley
Substantia Nigra
Tyrosine 3-Monooxygenase
Up-Regulation

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

24006664

Citation

TY - JOUR T1 - [Effect of electroacupuncture stimulation of "Fengfu" (GV 16) and "Taichong" (LR 3) on expression of COX-2 and tyrosine hydroxylase in Substantia Nigra in rats with Parkinson's disease]. AU - Wang,Shu-Ju, AU - Fang,Jian-Qiao, AU - Ma,Jun, AU - Zhou,Dan, AU - Wang,Yan-Chun, AU - Gan,Shui-Yong, AU - Sun,Guo-Jie, PY - 2013/9/7/entrez PY - 2013/9/7/pubmed PY - 2013/9/26/medline SP - 198 EP - 201 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 38 IS - 3 N2 - OBJECTIVE: To observe the influence of electroacupuncture (EA) therapy on the expression of tyrosine hydroxylase (TH) and cyclooxygenase-2 (COX-2) proteins of Substantia Nigra cells(SNc) in the rotenone-induced Parkinson's disease(PD) rats, so as to explore the mechanism of EA underlying improvement of PD. METHODS: Forty rats were randomly divided into normal, sham-operation (sham), model and EA groups (n = 10/group). The PD model was established by successive subcutaneous injection of rotenone (highly selective lesions of nigrostriatal dopaminergic neurons) for 28 days. EA (2 Hz, 1 mA) was applied to bilateral "Fengfu" (GV 16) and "Taichong" (LR 3) for 20 min, once daily for 14 days. The expression levels of TH and COX-2 proteins in the Substantia Nigra of midbrain were detected with Western blotting. RESULTS: Compared with the normal group, the expression level of TH protein in the model group was significantly decreased (P < 0.01), and that of COX-2 protein in the model group was significantly increased (P < 0.01). After the EA treatment, the expression level of TH in the EA group was obviously upregulated (P < 0.01), and that of COX-2 protein in the EA group was considerably down-regulated (P < 0.01). No significant differences were found between the normal and sham groups in the expression levels of TH and COX-2 proteins (P > 0.05). CONCLUSION: EA therapy can decrease inflammation mediator COX-2 protein expression and upregulate TH protein expression in the Substantia Nigra of midbrain in PD rats, which may contribute to its effect in relieving PD in clinic. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/24006664/[Effect_of_electroacupuncture_stimulation_of_"Fengfu"__GV_16__and_"Taichong"__LR_3__on_expression_of_COX_2_and_tyrosine_hydroxylase_in_Substantia_Nigra_in_rats_with_Parkinson's_disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html ER -