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Poultry as reservoir for extraintestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans.
Vet Microbiol. 2013 Dec 27; 167(3-4):506-12.VM

Abstract

Escherichia coli strains O45:K1:H7 are implicated in severe human infections such as meningitis. Since an increasing prevalence of serogroup O45 among avian pathogenic (APEC) and human extraintestinal pathogenic (ExPEC) E. coli strains isolated in Spain have been noticed, the aims of the present study were to investigate similarities between poultry and human O45 isolates, and to investigate the evolutionary relationship of ST95 types. The genetic relatedness and virulence gene profiles of 55 O45 APEC obtained from an avian colibacillosis collection (1991-2011) and 19 human O45 ExPEC from a human septicemic/uropathogenic (UPEC) E. coli collection (1989-2010) were determined by multilocus sequence typing (MLST), pulsed-field-gel-electrophoresis (PFGE), ECOR phylogrouping, and PCR-based genotyping. Two main clonal groups were established. The most prevalent and highly pathogenic O45:K1:H7-B2-ST95 shows a successful persistence since the 90s to the present, with parallel evolution both in human and poultry, on the basis of their PFGE and virulence gene profile similarities (9 human strains and 15 avian strains showed ≥85% PFGE identity). Comparison of this group with other ST95 closely related members (O1:K1:H7 and O18:K1:H7 isolates from our collections) shows pathogenic specialization through conserved virulence genotypes. The other prevalent O45 clonal group characterized in this study, the O45:HNM/H19-D-ST371/ST2676 was only detected in APEC strains suggesting host specificity. In conclusion, poultry could be acting as a reservoir of O45:K1:H7-B2-ST95 and other pathogenic ST95 serotypes in humans. Further studies would be necessary to clarify if pathogenic mechanisms used by ST95 strains are the same in avian and human hosts.

Authors+Show Affiliations

Department of Microbiology and Parasitology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain. Electronic address: azucena.mora@usc.es.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24008093

Citation

Mora, Azucena, et al. "Poultry as Reservoir for Extraintestinal Pathogenic Escherichia Coli O45:K1:H7-B2-ST95 in Humans." Veterinary Microbiology, vol. 167, no. 3-4, 2013, pp. 506-12.
Mora A, Viso S, López C, et al. Poultry as reservoir for extraintestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans. Vet Microbiol. 2013;167(3-4):506-12.
Mora, A., Viso, S., López, C., Alonso, M. P., García-Garrote, F., Dabhi, G., Mamani, R., Herrera, A., Marzoa, J., Blanco, M., Blanco, J. E., Moulin-Schouleur, M., Schouler, C., & Blanco, J. (2013). Poultry as reservoir for extraintestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans. Veterinary Microbiology, 167(3-4), 506-12. https://doi.org/10.1016/j.vetmic.2013.08.007
Mora A, et al. Poultry as Reservoir for Extraintestinal Pathogenic Escherichia Coli O45:K1:H7-B2-ST95 in Humans. Vet Microbiol. 2013 Dec 27;167(3-4):506-12. PubMed PMID: 24008093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poultry as reservoir for extraintestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans. AU - Mora,Azucena, AU - Viso,Susana, AU - López,Cecilia, AU - Alonso,María Pilar, AU - García-Garrote,Fernando, AU - Dabhi,Ghizlane, AU - Mamani,Rosalía, AU - Herrera,Alexandra, AU - Marzoa,Juan, AU - Blanco,Miguel, AU - Blanco,Jesús E, AU - Moulin-Schouleur,Maryvonne, AU - Schouler,Catherine, AU - Blanco,Jorge, Y1 - 2013/08/18/ PY - 2013/04/11/received PY - 2013/06/13/revised PY - 2013/08/02/accepted PY - 2013/9/7/entrez PY - 2013/9/7/pubmed PY - 2014/5/27/medline KW - APEC KW - Escherichia coli KW - ExPEC KW - NMEC KW - O45 KW - ST95 SP - 506 EP - 12 JF - Veterinary microbiology JO - Vet Microbiol VL - 167 IS - 3-4 N2 - Escherichia coli strains O45:K1:H7 are implicated in severe human infections such as meningitis. Since an increasing prevalence of serogroup O45 among avian pathogenic (APEC) and human extraintestinal pathogenic (ExPEC) E. coli strains isolated in Spain have been noticed, the aims of the present study were to investigate similarities between poultry and human O45 isolates, and to investigate the evolutionary relationship of ST95 types. The genetic relatedness and virulence gene profiles of 55 O45 APEC obtained from an avian colibacillosis collection (1991-2011) and 19 human O45 ExPEC from a human septicemic/uropathogenic (UPEC) E. coli collection (1989-2010) were determined by multilocus sequence typing (MLST), pulsed-field-gel-electrophoresis (PFGE), ECOR phylogrouping, and PCR-based genotyping. Two main clonal groups were established. The most prevalent and highly pathogenic O45:K1:H7-B2-ST95 shows a successful persistence since the 90s to the present, with parallel evolution both in human and poultry, on the basis of their PFGE and virulence gene profile similarities (9 human strains and 15 avian strains showed ≥85% PFGE identity). Comparison of this group with other ST95 closely related members (O1:K1:H7 and O18:K1:H7 isolates from our collections) shows pathogenic specialization through conserved virulence genotypes. The other prevalent O45 clonal group characterized in this study, the O45:HNM/H19-D-ST371/ST2676 was only detected in APEC strains suggesting host specificity. In conclusion, poultry could be acting as a reservoir of O45:K1:H7-B2-ST95 and other pathogenic ST95 serotypes in humans. Further studies would be necessary to clarify if pathogenic mechanisms used by ST95 strains are the same in avian and human hosts. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/24008093/Poultry_as_reservoir_for_extraintestinal_pathogenic_Escherichia_coli_O45:K1:H7_B2_ST95_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(13)00406-9 DB - PRIME DP - Unbound Medicine ER -