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In situ Raman study of redox state changes of mitochondrial cytochromes in a perfused rat heart.
PLoS One 2013; 8(8):e70488Plos

Abstract

We developed a Raman spectroscopy-based approach for simultaneous study of redox changes in c-and b-type cytochromes and for a semiquantitative estimation of the amount of oxygenated myoglobin in a perfused rat heart. Excitation at 532 nm was used to obtain Raman scattering of the myocardial surface of the isolated heart at normal and hypoxic conditions. Raman spectra of the heart under normal pO2 demonstrate unique peaks attributable to reduced c-and b-type cytochromes and oxymyoglobin (oMb). The cytochrome peaks decreased in intensity upon FCCP treatment, as predicted from uncoupling mitochondrial respiration. Conversely, transient hypoxia causes the reversible increase in the intensity of peaks assigned to cytochromes c and c1, reflecting electron stacking proximal to cytochrome oxidase due to the lack of terminal electron acceptor O2. Intensities of peaks assigned to oxy- and deoxyhemoglobin were used for the semiquantitative estimation of oMb deoxygenation that was found to be of approximately 50[Formula: see text] under hypoxia conditions.

Authors+Show Affiliations

Biophysics Department, Biological faculty, Moscow State University, Moscow, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24009655

Citation

Brazhe, Nadezda A., et al. "In Situ Raman Study of Redox State Changes of Mitochondrial Cytochromes in a Perfused Rat Heart." PloS One, vol. 8, no. 8, 2013, pp. e70488.
Brazhe NA, Treiman M, Faricelli B, et al. In situ Raman study of redox state changes of mitochondrial cytochromes in a perfused rat heart. PLoS ONE. 2013;8(8):e70488.
Brazhe, N. A., Treiman, M., Faricelli, B., Vestergaard, J. H., & Sosnovtseva, O. (2013). In situ Raman study of redox state changes of mitochondrial cytochromes in a perfused rat heart. PloS One, 8(8), pp. e70488. doi:10.1371/journal.pone.0070488.
Brazhe NA, et al. In Situ Raman Study of Redox State Changes of Mitochondrial Cytochromes in a Perfused Rat Heart. PLoS ONE. 2013;8(8):e70488. PubMed PMID: 24009655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ Raman study of redox state changes of mitochondrial cytochromes in a perfused rat heart. AU - Brazhe,Nadezda A, AU - Treiman,Marek, AU - Faricelli,Barbara, AU - Vestergaard,Jakob H, AU - Sosnovtseva,Olga, Y1 - 2013/08/29/ PY - 2013/04/16/received PY - 2013/06/19/accepted PY - 2013/9/7/entrez PY - 2013/9/7/pubmed PY - 2014/4/17/medline SP - e70488 EP - e70488 JF - PloS one JO - PLoS ONE VL - 8 IS - 8 N2 - We developed a Raman spectroscopy-based approach for simultaneous study of redox changes in c-and b-type cytochromes and for a semiquantitative estimation of the amount of oxygenated myoglobin in a perfused rat heart. Excitation at 532 nm was used to obtain Raman scattering of the myocardial surface of the isolated heart at normal and hypoxic conditions. Raman spectra of the heart under normal pO2 demonstrate unique peaks attributable to reduced c-and b-type cytochromes and oxymyoglobin (oMb). The cytochrome peaks decreased in intensity upon FCCP treatment, as predicted from uncoupling mitochondrial respiration. Conversely, transient hypoxia causes the reversible increase in the intensity of peaks assigned to cytochromes c and c1, reflecting electron stacking proximal to cytochrome oxidase due to the lack of terminal electron acceptor O2. Intensities of peaks assigned to oxy- and deoxyhemoglobin were used for the semiquantitative estimation of oMb deoxygenation that was found to be of approximately 50[Formula: see text] under hypoxia conditions. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24009655/In_situ_Raman_study_of_redox_state_changes_of_mitochondrial_cytochromes_in_a_perfused_rat_heart_ L2 - http://dx.plos.org/10.1371/journal.pone.0070488 DB - PRIME DP - Unbound Medicine ER -