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Polymer-based alternative method to extract bromelain from pineapple peel waste.
Biotechnol Appl Biochem 2013 Sep-Oct; 60(5):527-35BA

Abstract

Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.

Authors+Show Affiliations

Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24011234

Citation

Novaes, Letícia Celia de Lencastre, et al. "Polymer-based Alternative Method to Extract Bromelain From Pineapple Peel Waste." Biotechnology and Applied Biochemistry, vol. 60, no. 5, 2013, pp. 527-35.
Novaes LC, Ebinuma Vde C, Mazzola PG, et al. Polymer-based alternative method to extract bromelain from pineapple peel waste. Biotechnol Appl Biochem. 2013;60(5):527-35.
Novaes, L. C., Ebinuma, V. d. e. . C., Mazzola, P. G., & Pessoa, A. (2013). Polymer-based alternative method to extract bromelain from pineapple peel waste. Biotechnology and Applied Biochemistry, 60(5), pp. 527-35. doi:10.1002/bab.1121.
Novaes LC, et al. Polymer-based Alternative Method to Extract Bromelain From Pineapple Peel Waste. Biotechnol Appl Biochem. 2013;60(5):527-35. PubMed PMID: 24011234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymer-based alternative method to extract bromelain from pineapple peel waste. AU - Novaes,Letícia Celia de Lencastre, AU - Ebinuma,Valéria de Carvalho Santos, AU - Mazzola,Priscila Gava, AU - Pessoa,Adalberto,Jr Y1 - 2013/09/06/ PY - 2013/03/13/received PY - 2013/04/23/accepted PY - 2013/9/10/entrez PY - 2013/9/10/pubmed PY - 2014/12/17/medline KW - aqueous two-phase system KW - bromelain KW - pineapple waste KW - poly(acrylic acid) KW - polyethylene glycol SP - 527 EP - 35 JF - Biotechnology and applied biochemistry JO - Biotechnol. Appl. Biochem. VL - 60 IS - 5 N2 - Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process. SN - 1470-8744 UR - https://www.unboundmedicine.com/medline/citation/24011234/Polymer_based_alternative_method_to_extract_bromelain_from_pineapple_peel_waste_ L2 - https://doi.org/10.1002/bab.1121 DB - PRIME DP - Unbound Medicine ER -