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Single-cell gel electrophoresis assay in the ten spotted live-bearer fish, Cnesterodon decemmaculatus (Jenyns, 1842), as bioassay for agrochemical-induced genotoxicity.
Ecotoxicol Environ Saf. 2013 Dec; 98:368-73.EE

Abstract

The ability of two 48 percent chlorpyrifos-based insecticides (Lorsban* 48E® and CPF Zamba®), two 50 percent pirimicarb-based insecticides (Aficida® and Patton Flow®), and two 48 percent glyphosate-based herbicides (Panzer® and Credit®) to induce DNA single-strand breaks in peripheral blood erythrocytes of Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae) exposed under laboratory conditions was evaluated by the single-cell gel electrophoresis (SCGE) assay. In those fish exposed to Lorsban* 48E®, CPF Zamba®, Aficida®, Patton Flow®, Credit®, and Panzer®, a significant increase of the genetic damage was observed for all formulations regardless of the harvesting time. This genotoxic effect was achieved by an enhancement of Type II-IV comets and a concomitant decrease of Type 0-I comets over control values. A regression analysis revealed that the damage varied as a negative function of the exposure time in those Lorsban* 48E®- and Aficida®-treated fish. On the other hand, a positive correlation between damage increase and exposure time was achieved after Patton Flow® and Credit® treatment. Finally, no correlation was observed between increase in the genetic damage and exposure time after treatment with CPF Zamba® or Panzer®. These results highlight that all agrochemicals inflict primary genotoxic damage at the DNA level at sublethal concentrations, regardless of the exposure time of the aquatic organisms under study, at least within a period of 96 h of treatment.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24011534

Citation

Vera-Candioti, Josefina, et al. "Single-cell Gel Electrophoresis Assay in the Ten Spotted Live-bearer Fish, Cnesterodon Decemmaculatus (Jenyns, 1842), as Bioassay for Agrochemical-induced Genotoxicity." Ecotoxicology and Environmental Safety, vol. 98, 2013, pp. 368-73.
Vera-Candioti J, Soloneski S, Larramendy ML. Single-cell gel electrophoresis assay in the ten spotted live-bearer fish, Cnesterodon decemmaculatus (Jenyns, 1842), as bioassay for agrochemical-induced genotoxicity. Ecotoxicol Environ Saf. 2013;98:368-73.
Vera-Candioti, J., Soloneski, S., & Larramendy, M. L. (2013). Single-cell gel electrophoresis assay in the ten spotted live-bearer fish, Cnesterodon decemmaculatus (Jenyns, 1842), as bioassay for agrochemical-induced genotoxicity. Ecotoxicology and Environmental Safety, 98, 368-73. https://doi.org/10.1016/j.ecoenv.2013.08.011
Vera-Candioti J, Soloneski S, Larramendy ML. Single-cell Gel Electrophoresis Assay in the Ten Spotted Live-bearer Fish, Cnesterodon Decemmaculatus (Jenyns, 1842), as Bioassay for Agrochemical-induced Genotoxicity. Ecotoxicol Environ Saf. 2013;98:368-73. PubMed PMID: 24011534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-cell gel electrophoresis assay in the ten spotted live-bearer fish, Cnesterodon decemmaculatus (Jenyns, 1842), as bioassay for agrochemical-induced genotoxicity. AU - Vera-Candioti,Josefina, AU - Soloneski,Sonia, AU - Larramendy,Marcelo L, Y1 - 2013/09/05/ PY - 2013/05/03/received PY - 2013/08/07/revised PY - 2013/08/13/accepted PY - 2013/9/10/entrez PY - 2013/9/10/pubmed PY - 2014/3/25/medline KW - Agrochemical commercial formulations KW - Chlorpyrifos KW - Comet assay KW - Glyphosate KW - Pirimicarb KW - Poeciliidae SP - 368 EP - 73 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 98 N2 - The ability of two 48 percent chlorpyrifos-based insecticides (Lorsban* 48E® and CPF Zamba®), two 50 percent pirimicarb-based insecticides (Aficida® and Patton Flow®), and two 48 percent glyphosate-based herbicides (Panzer® and Credit®) to induce DNA single-strand breaks in peripheral blood erythrocytes of Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae) exposed under laboratory conditions was evaluated by the single-cell gel electrophoresis (SCGE) assay. In those fish exposed to Lorsban* 48E®, CPF Zamba®, Aficida®, Patton Flow®, Credit®, and Panzer®, a significant increase of the genetic damage was observed for all formulations regardless of the harvesting time. This genotoxic effect was achieved by an enhancement of Type II-IV comets and a concomitant decrease of Type 0-I comets over control values. A regression analysis revealed that the damage varied as a negative function of the exposure time in those Lorsban* 48E®- and Aficida®-treated fish. On the other hand, a positive correlation between damage increase and exposure time was achieved after Patton Flow® and Credit® treatment. Finally, no correlation was observed between increase in the genetic damage and exposure time after treatment with CPF Zamba® or Panzer®. These results highlight that all agrochemicals inflict primary genotoxic damage at the DNA level at sublethal concentrations, regardless of the exposure time of the aquatic organisms under study, at least within a period of 96 h of treatment. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/24011534/Single_cell_gel_electrophoresis_assay_in_the_ten_spotted_live_bearer_fish_Cnesterodon_decemmaculatus__Jenyns_1842__as_bioassay_for_agrochemical_induced_genotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(13)00355-2 DB - PRIME DP - Unbound Medicine ER -