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Effects of potential probiotic Bacillus amyloliquefaciens [corrected] FPTB16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (Catla catla).
Fish Shellfish Immunol. 2013 Nov; 35(5):1547-53.FS

Abstract

Effects of dietary administration of Bacillus amyloliquefaciens FPTB16 on systemic and mucosal immunity and disease resistance of catla (Catla catla) against Edwardsiella tarda infection were evaluated in the present study. The laboratory maintained B. amyloliquefaciens was used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. Healthy catla were challenged by this bacterium for determination of its safety. For preparation of probiotic supplemented diet, the bacteria were added to the basal diet (control) at three different inclusion levels i.e., 1 × 10(9), 1 × 10(8) and 1 × 10(7) CFU/g diet. Fish (weight 25-30 g) were fed with these diets and various immune parameters and disease resistance study were conducted at 4 weeks and 8 weeks post-feeding. The bacterial antagonism study showed inhibition zone against E. tarda, Aeromonas hydrophila, Vibrio parahaemolyticus and V. harveyi. B. amyloliquefaciens was harmless to catla as neither mortalities nor morbidities were observed after the challenge. Study of different systemic and mucosal immunological parameters viz. superoxide anion production and nitric oxide production, myeloperoxidase content, lysozyme activity and total protein content showed significant enhancement (p < 0.05) in fish fed with 10(8) and 10(9) CFU/g B. amyloliquefaciens at both time points with the highest values observed in case of 10(9) CFU/g. For fish fed with 10(7) CFU/g B. amyloliquefaciens, all the parameters showed significant enhancement (p < 0.05) at both time points except the lysozyme activity of serum at 8 weeks. Diet containing 10(8) and 10(9) CFU/g B. amyloliquefaciens significantly enhanced (p < 0.05) the resistance of catla against bacterial challenge at both time points. These results collectively suggest that B. amyloliquefaciens is a potential probiotic species and can be used in aquaculture to improve health status and disease resistance with an optimal dietary supplementation of 10(9) CFU/g.

Authors+Show Affiliations

Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, Lembucherra, Post Box No. 60, Agartala 799 001, Tripura, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24012512

Citation

Das, Anushree, et al. "Effects of Potential Probiotic Bacillus Amyloliquefaciens [corrected] FPTB16 On Systemic and Cutaneous Mucosal Immune Responses and Disease Resistance of Catla (Catla Catla)." Fish & Shellfish Immunology, vol. 35, no. 5, 2013, pp. 1547-53.
Das A, Nakhro K, Chowdhury S, et al. Effects of potential probiotic Bacillus amyloliquefaciens [corrected] FPTB16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (Catla catla). Fish Shellfish Immunol. 2013;35(5):1547-53.
Das, A., Nakhro, K., Chowdhury, S., & Kamilya, D. (2013). Effects of potential probiotic Bacillus amyloliquefaciens [corrected] FPTB16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (Catla catla). Fish & Shellfish Immunology, 35(5), 1547-53. https://doi.org/10.1016/j.fsi.2013.08.022
Das A, et al. Effects of Potential Probiotic Bacillus Amyloliquefaciens [corrected] FPTB16 On Systemic and Cutaneous Mucosal Immune Responses and Disease Resistance of Catla (Catla Catla). Fish Shellfish Immunol. 2013;35(5):1547-53. PubMed PMID: 24012512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of potential probiotic Bacillus amyloliquefaciens [corrected] FPTB16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (Catla catla). AU - Das,Anushree, AU - Nakhro,Khriezhato, AU - Chowdhury,Supratim, AU - Kamilya,Dibyendu, Y1 - 2013/09/04/ PY - 2013/06/21/received PY - 2013/08/12/revised PY - 2013/08/27/accepted PY - 2013/9/10/entrez PY - 2013/9/10/pubmed PY - 2014/5/10/medline KW - Bacillus amyloliquefaciens FPTB16 KW - Catla (Catla catla) KW - Disease resistance KW - Immune response KW - Probiotic SP - 1547 EP - 53 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 35 IS - 5 N2 - Effects of dietary administration of Bacillus amyloliquefaciens FPTB16 on systemic and mucosal immunity and disease resistance of catla (Catla catla) against Edwardsiella tarda infection were evaluated in the present study. The laboratory maintained B. amyloliquefaciens was used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. Healthy catla were challenged by this bacterium for determination of its safety. For preparation of probiotic supplemented diet, the bacteria were added to the basal diet (control) at three different inclusion levels i.e., 1 × 10(9), 1 × 10(8) and 1 × 10(7) CFU/g diet. Fish (weight 25-30 g) were fed with these diets and various immune parameters and disease resistance study were conducted at 4 weeks and 8 weeks post-feeding. The bacterial antagonism study showed inhibition zone against E. tarda, Aeromonas hydrophila, Vibrio parahaemolyticus and V. harveyi. B. amyloliquefaciens was harmless to catla as neither mortalities nor morbidities were observed after the challenge. Study of different systemic and mucosal immunological parameters viz. superoxide anion production and nitric oxide production, myeloperoxidase content, lysozyme activity and total protein content showed significant enhancement (p < 0.05) in fish fed with 10(8) and 10(9) CFU/g B. amyloliquefaciens at both time points with the highest values observed in case of 10(9) CFU/g. For fish fed with 10(7) CFU/g B. amyloliquefaciens, all the parameters showed significant enhancement (p < 0.05) at both time points except the lysozyme activity of serum at 8 weeks. Diet containing 10(8) and 10(9) CFU/g B. amyloliquefaciens significantly enhanced (p < 0.05) the resistance of catla against bacterial challenge at both time points. These results collectively suggest that B. amyloliquefaciens is a potential probiotic species and can be used in aquaculture to improve health status and disease resistance with an optimal dietary supplementation of 10(9) CFU/g. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/24012512/Effects_of_potential_probiotic_Bacillus_amyloliquefaciens_[corrected]_FPTB16_on_systemic_and_cutaneous_mucosal_immune_responses_and_disease_resistance_of_catla__Catla_catla__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(13)00724-9 DB - PRIME DP - Unbound Medicine ER -