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ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides.
Gene. 2013 Dec 01; 531(2):318-25.GENE

Abstract

High-molecular-weight glutenin subunits (HMW-GSs) are of considerable interest, because they play a crucial role in determining dough viscoelastic properties and end-use quality of wheat flour. In this paper, ChAy/Bx, a novel chimeric HMW-GS gene from Triticum turgidum ssp. dicoccoides (AABB, 2n=4x=28) accession D129, was isolated and characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the electrophoretic mobility of the glutenin subunit encoded by ChAy/Bx was slightly faster than that of 1Dy12. The complete ORF of ChAy/Bx contained 1,671 bp encoding a deduced polypeptide of 555 amino acid residues (or 534 amino acid residues for the mature protein), making it the smallest HMW-GS gene known from Triticum species. Sequence analysis showed that ChAy/Bx was neither a conventional x-type nor a conventional y-type subunit gene, but a novel chimeric gene. Its first 1305 nt sequence was highly homologous with the corresponding sequence of 1Ay type genes, while its final 366 nt sequence was highly homologous with the corresponding sequence of 1Bx type genes. The mature ChAy/Bx protein consisted of the N-terminus of 1Ay type subunit (the first 414 amino acid residues) and the C-terminus of 1Bx type subunit (the final 120 amino acid residues). Secondary structure prediction showed that ChAy/Bx contained some domains of 1Ay subunit and some domains of 1Bx subunit. The special structure of this HMW glutenin chimera ChAy/Bx subunit might have unique effects on the end-use quality of wheat flour. Here we propose that homoeologous recombination might be a novel pathway for allelic variation or molecular evolution of HMW-GSs.

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Authors+Show Affiliations

Guo XH
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an 625014, China.
Bi ZG
No affiliation info available
Wu BH
No affiliation info available
Wang ZZ
No affiliation info available
Hu JL
No affiliation info available
Zheng YL
No affiliation info available
Liu DC
No affiliation info available

MeSH

Base SequenceCloning, MolecularEvolution, MolecularGenes, PlantGlutensHomologous RecombinationMolecular Sequence DataMolecular WeightMutant Chimeric ProteinsOpen Reading FramesProtein SubunitsSequence Analysis, DNASequence Homology, Nucleic AcidTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24012818

Citation

Guo, Xiao-Hui, et al. "ChAy/Bx, a Novel Chimeric High-molecular-weight Glutenin Subunit Gene Apparently Created By Homoeologous Recombination in Triticum Turgidum Ssp. Dicoccoides." Gene, vol. 531, no. 2, 2013, pp. 318-25.
Guo XH, Bi ZG, Wu BH, et al. ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides. Gene. 2013;531(2):318-25.
Guo, X. H., Bi, Z. G., Wu, B. H., Wang, Z. Z., Hu, J. L., Zheng, Y. L., & Liu, D. C. (2013). ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides. Gene, 531(2), 318-25. https://doi.org/10.1016/j.gene.2013.08.073
Guo XH, et al. ChAy/Bx, a Novel Chimeric High-molecular-weight Glutenin Subunit Gene Apparently Created By Homoeologous Recombination in Triticum Turgidum Ssp. Dicoccoides. Gene. 2013 Dec 1;531(2):318-25. PubMed PMID: 24012818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides. AU - Guo,Xiao-Hui, AU - Bi,Zhe-Guang, AU - Wu,Bi-Hua, AU - Wang,Zhen-Zhen, AU - Hu,Ji-Liang, AU - Zheng,You-Liang, AU - Liu,Deng-Cai, Y1 - 2013/09/04/ PY - 2013/03/25/received PY - 2013/08/14/revised PY - 2013/08/21/accepted PY - 2013/9/10/entrez PY - 2013/9/10/pubmed PY - 2013/12/24/medline KW - Allelic variation KW - CTAB KW - Chimeric gene KW - Evolution KW - GISH KW - HMW-GS KW - High-molecular-weight glutenin subunit KW - Homoeologous recombination KW - IPTG KW - InDels KW - LMW-GS KW - NCBI KW - National Center for Biotechnology Information KW - ORF KW - PCR KW - SDS-PAGE KW - SNP KW - Triticum turgidum ssp. dicoccoides KW - aa KW - amino acid(s) KW - base pair(s) KW - bp KW - cetyltrimethyl ammonium bromide KW - dNTPs KW - deoxyribonucleoside triphosphates KW - genomic in situ hybridization KW - high-molecular-weight glutenin subunit KW - insertions and deletions KW - isopropyl-β-Δ-thiogalactopyranoside KW - low-molecular-weight glutenin subunit KW - open reading frame KW - polymerase chain reaction KW - single nucleotide polymorphism KW - sodium dodecyl sulfate polyacrylamide gel electrophoresis SP - 318 EP - 25 JF - Gene JO - Gene VL - 531 IS - 2 N2 - High-molecular-weight glutenin subunits (HMW-GSs) are of considerable interest, because they play a crucial role in determining dough viscoelastic properties and end-use quality of wheat flour. In this paper, ChAy/Bx, a novel chimeric HMW-GS gene from Triticum turgidum ssp. dicoccoides (AABB, 2n=4x=28) accession D129, was isolated and characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the electrophoretic mobility of the glutenin subunit encoded by ChAy/Bx was slightly faster than that of 1Dy12. The complete ORF of ChAy/Bx contained 1,671 bp encoding a deduced polypeptide of 555 amino acid residues (or 534 amino acid residues for the mature protein), making it the smallest HMW-GS gene known from Triticum species. Sequence analysis showed that ChAy/Bx was neither a conventional x-type nor a conventional y-type subunit gene, but a novel chimeric gene. Its first 1305 nt sequence was highly homologous with the corresponding sequence of 1Ay type genes, while its final 366 nt sequence was highly homologous with the corresponding sequence of 1Bx type genes. The mature ChAy/Bx protein consisted of the N-terminus of 1Ay type subunit (the first 414 amino acid residues) and the C-terminus of 1Bx type subunit (the final 120 amino acid residues). Secondary structure prediction showed that ChAy/Bx contained some domains of 1Ay subunit and some domains of 1Bx subunit. The special structure of this HMW glutenin chimera ChAy/Bx subunit might have unique effects on the end-use quality of wheat flour. Here we propose that homoeologous recombination might be a novel pathway for allelic variation or molecular evolution of HMW-GSs. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24012818/ChAy/Bx_a_novel_chimeric_high_molecular_weight_glutenin_subunit_gene_apparently_created_by_homoeologous_recombination_in_Triticum_turgidum_ssp__dicoccoides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(13)01133-5 DB - PRIME DP - Unbound Medicine ER -