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Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria.
Microbiologyopen 2013; 2(6):893-900M

Abstract

Chloromethane (CH3 Cl) is a widely studied volatile halocarbon involved in the destruction of ozone in the stratosphere. Nevertheless, its global budget still remains debated. Stable isotope analysis is a powerful tool to constrain fluxes of chloromethane between various environmental compartments which involve a multiplicity of sources and sinks, and both biotic and abiotic processes. In this study, we measured hydrogen and carbon isotope fractionation of the remaining untransformed chloromethane following its degradation by methylotrophic bacterial strains Methylobacterium extorquens CM4 and Hyphomicrobium sp. MC1, which belong to different genera but both use the cmu pathway, the only pathway for bacterial degradation of chloromethane characterized so far. Hydrogen isotope fractionation for degradation of chloromethane was determined for the first time, and yielded enrichment factors (ε) of -29‰ and -27‰ for strains CM4 and MC1, respectively. In agreement with previous studies, enrichment in (13) C of untransformed CH3 Cl was also observed, and similar isotope enrichment factors (ε) of -41‰ and -38‰ were obtained for degradation of chloromethane by strains CM4 and MC1, respectively. These combined hydrogen and carbon isotopic data for bacterial degradation of chloromethane will contribute to refine models of the global atmospheric budget of chloromethane.

Authors+Show Affiliations

Equipe Adaptations et Interactions Microbiennes dans l'Environnement, UMR 7156 Université de Strasbourg - CNRS, 28 rue Goethe, Strasbourg, 67083, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24019296

Citation

Nadalig, Thierry, et al. "Hydrogen and Carbon Isotope Fractionation During Degradation of Chloromethane By Methylotrophic Bacteria." MicrobiologyOpen, vol. 2, no. 6, 2013, pp. 893-900.
Nadalig T, Greule M, Bringel F, et al. Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria. Microbiologyopen. 2013;2(6):893-900.
Nadalig, T., Greule, M., Bringel, F., Vuilleumier, S., & Keppler, F. (2013). Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria. MicrobiologyOpen, 2(6), pp. 893-900. doi:10.1002/mbo3.124.
Nadalig T, et al. Hydrogen and Carbon Isotope Fractionation During Degradation of Chloromethane By Methylotrophic Bacteria. Microbiologyopen. 2013;2(6):893-900. PubMed PMID: 24019296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria. AU - Nadalig,Thierry, AU - Greule,Markus, AU - Bringel,Françoise, AU - Vuilleumier,Stéphane, AU - Keppler,Frank, Y1 - 2013/09/08/ PY - 2013/02/28/received PY - 2013/07/10/revised PY - 2013/07/21/accepted PY - 2013/9/11/entrez PY - 2013/9/11/pubmed PY - 2014/7/23/medline KW - Carbon isotope fractionation KW - chloromethane biodegradation KW - hydrogen isotope fractionation KW - methylotrophic bacteria SP - 893 EP - 900 JF - MicrobiologyOpen JO - Microbiologyopen VL - 2 IS - 6 N2 - Chloromethane (CH3 Cl) is a widely studied volatile halocarbon involved in the destruction of ozone in the stratosphere. Nevertheless, its global budget still remains debated. Stable isotope analysis is a powerful tool to constrain fluxes of chloromethane between various environmental compartments which involve a multiplicity of sources and sinks, and both biotic and abiotic processes. In this study, we measured hydrogen and carbon isotope fractionation of the remaining untransformed chloromethane following its degradation by methylotrophic bacterial strains Methylobacterium extorquens CM4 and Hyphomicrobium sp. MC1, which belong to different genera but both use the cmu pathway, the only pathway for bacterial degradation of chloromethane characterized so far. Hydrogen isotope fractionation for degradation of chloromethane was determined for the first time, and yielded enrichment factors (ε) of -29‰ and -27‰ for strains CM4 and MC1, respectively. In agreement with previous studies, enrichment in (13) C of untransformed CH3 Cl was also observed, and similar isotope enrichment factors (ε) of -41‰ and -38‰ were obtained for degradation of chloromethane by strains CM4 and MC1, respectively. These combined hydrogen and carbon isotopic data for bacterial degradation of chloromethane will contribute to refine models of the global atmospheric budget of chloromethane. SN - 2045-8827 UR - https://www.unboundmedicine.com/medline/citation/24019296/Hydrogen_and_carbon_isotope_fractionation_during_degradation_of_chloromethane_by_methylotrophic_bacteria_ L2 - https://doi.org/10.1002/mbo3.124 DB - PRIME DP - Unbound Medicine ER -