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Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay.
Am J Trop Med Hyg. 2013 Nov; 89(5):971-972.AJ

Abstract

Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24019434

Citation

Chantratita, Narisara, et al. "Rapid Detection of Burkholderia Pseudomallei in Blood Cultures Using a Monoclonal Antibody-based Immunofluorescent Assay." The American Journal of Tropical Medicine and Hygiene, vol. 89, no. 5, 2013, pp. 971-972.
Chantratita N, Tandhavanant S, Wongsuvan G, et al. Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. Am J Trop Med Hyg. 2013;89(5):971-972.
Chantratita, N., Tandhavanant, S., Wongsuvan, G., Wuthiekanun, V., Teerawattanasook, N., Day, N. P. J., Limmathurotsakul, D., & Peacock, S. J. (2013). Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. The American Journal of Tropical Medicine and Hygiene, 89(5), 971-972. https://doi.org/10.4269/ajtmh.13-0212
Chantratita N, et al. Rapid Detection of Burkholderia Pseudomallei in Blood Cultures Using a Monoclonal Antibody-based Immunofluorescent Assay. Am J Trop Med Hyg. 2013;89(5):971-972. PubMed PMID: 24019434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. AU - Chantratita,Narisara, AU - Tandhavanant,Sarunporn, AU - Wongsuvan,Gumphol, AU - Wuthiekanun,Vanaporn, AU - Teerawattanasook,Nittaya, AU - Day,Nicholas P J, AU - Limmathurotsakul,Direk, AU - Peacock,Sharon J, Y1 - 2013/09/09/ PY - 2013/9/11/entrez PY - 2013/9/11/pubmed PY - 2013/12/29/medline SP - 971 EP - 972 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 89 IS - 5 N2 - Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/24019434/Rapid_detection_of_Burkholderia_pseudomallei_in_blood_cultures_using_a_monoclonal_antibody_based_immunofluorescent_assay_ L2 - http://www.ajtmh.org/content/journals/10.4269/ajtmh.13-0212?crawler=true&mimetype=application/pdf DB - PRIME DP - Unbound Medicine ER -