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Prolactin regulates TRPV1, TRPA1, and TRPM8 in sensory neurons in a sex-dependent manner: Contribution of prolactin receptor to inflammatory pain.
Am J Physiol Endocrinol Metab. 2013 Nov 01; 305(9):E1154-64.AJ

Abstract

Prolactin (PRL) is a hormone produced in the anterior pituitary but also synthesized extrapituitary where it can influence diverse cellular processes, including inflammatory responses. Females experience greater pain in certain inflammatory conditions, but the contribution of the PRL system to sex-dependent inflammatory pain is unknown. We found that PRL regulates transient receptor potential (TRP) channels in a sex-dependent manner in sensory neurons. At >20 ng/ml, PRL sensitizes TRPV1 in female, but not male, neurons. This effect is mediated by PRL receptor (PRL-R). Likewise, TRPA1 and TRPM8 were sensitized by 100 ng/ml PRL only in female neurons. We showed that complete Freund adjuvant (CFA) upregulated PRL levels in the inflamed paw of both male and female rats, but levels were higher in females. In contrast, CFA did not change mRNA levels of long and short PRL-R in the dorsal root ganglion or spinal cord. Analysis of PRL and PRL-R knockout (KO) mice demonstrated that basal responses to cold stimuli were only altered in females, and with no significant effects on heat and mechanical responses in both sexes. CFA-induced heat and cold hyperalgesia were not changed in PRL and PRL-R KO compared with wild-type (WT) males, whereas significant reduction of heat and cold post-CFA hyperalgesia was detected in PRL and PRL-R KO females. Attenuation of CFA-induced mechanical allodynia was observed in both PRL and PRL-R KO females and males. Thermal hyperalgesia in PRL KO females was restored by administration of PRL into hindpaws. Overall, we demonstrate a sex-dependent regulation of peripheral inflammatory hyperalgesia by the PRL system.

Authors+Show Affiliations

Department of Pharmacology University of Texas Health Science Center at San Antonio, San Antonio, Texas; and.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24022869

Citation

Patil, Mayur J., et al. "Prolactin Regulates TRPV1, TRPA1, and TRPM8 in Sensory Neurons in a Sex-dependent Manner: Contribution of Prolactin Receptor to Inflammatory Pain." American Journal of Physiology. Endocrinology and Metabolism, vol. 305, no. 9, 2013, pp. E1154-64.
Patil MJ, Ruparel SB, Henry MA, et al. Prolactin regulates TRPV1, TRPA1, and TRPM8 in sensory neurons in a sex-dependent manner: Contribution of prolactin receptor to inflammatory pain. Am J Physiol Endocrinol Metab. 2013;305(9):E1154-64.
Patil, M. J., Ruparel, S. B., Henry, M. A., & Akopian, A. N. (2013). Prolactin regulates TRPV1, TRPA1, and TRPM8 in sensory neurons in a sex-dependent manner: Contribution of prolactin receptor to inflammatory pain. American Journal of Physiology. Endocrinology and Metabolism, 305(9), E1154-64. https://doi.org/10.1152/ajpendo.00187.2013
Patil MJ, et al. Prolactin Regulates TRPV1, TRPA1, and TRPM8 in Sensory Neurons in a Sex-dependent Manner: Contribution of Prolactin Receptor to Inflammatory Pain. Am J Physiol Endocrinol Metab. 2013 Nov 1;305(9):E1154-64. PubMed PMID: 24022869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prolactin regulates TRPV1, TRPA1, and TRPM8 in sensory neurons in a sex-dependent manner: Contribution of prolactin receptor to inflammatory pain. AU - Patil,Mayur J, AU - Ruparel,Shivani B, AU - Henry,Michael A, AU - Akopian,Armen N, Y1 - 2013/09/10/ PY - 2013/9/12/entrez PY - 2013/9/12/pubmed PY - 2013/12/24/medline KW - female KW - inflammation KW - pain KW - prolactin receptor KW - transient receptor potential A1 KW - transient receptor potential M8 KW - transient receptor potential V1 SP - E1154 EP - 64 JF - American journal of physiology. Endocrinology and metabolism JO - Am J Physiol Endocrinol Metab VL - 305 IS - 9 N2 - Prolactin (PRL) is a hormone produced in the anterior pituitary but also synthesized extrapituitary where it can influence diverse cellular processes, including inflammatory responses. Females experience greater pain in certain inflammatory conditions, but the contribution of the PRL system to sex-dependent inflammatory pain is unknown. We found that PRL regulates transient receptor potential (TRP) channels in a sex-dependent manner in sensory neurons. At >20 ng/ml, PRL sensitizes TRPV1 in female, but not male, neurons. This effect is mediated by PRL receptor (PRL-R). Likewise, TRPA1 and TRPM8 were sensitized by 100 ng/ml PRL only in female neurons. We showed that complete Freund adjuvant (CFA) upregulated PRL levels in the inflamed paw of both male and female rats, but levels were higher in females. In contrast, CFA did not change mRNA levels of long and short PRL-R in the dorsal root ganglion or spinal cord. Analysis of PRL and PRL-R knockout (KO) mice demonstrated that basal responses to cold stimuli were only altered in females, and with no significant effects on heat and mechanical responses in both sexes. CFA-induced heat and cold hyperalgesia were not changed in PRL and PRL-R KO compared with wild-type (WT) males, whereas significant reduction of heat and cold post-CFA hyperalgesia was detected in PRL and PRL-R KO females. Attenuation of CFA-induced mechanical allodynia was observed in both PRL and PRL-R KO females and males. Thermal hyperalgesia in PRL KO females was restored by administration of PRL into hindpaws. Overall, we demonstrate a sex-dependent regulation of peripheral inflammatory hyperalgesia by the PRL system. SN - 1522-1555 UR - https://www.unboundmedicine.com/medline/citation/24022869/Prolactin_regulates_TRPV1_TRPA1_and_TRPM8_in_sensory_neurons_in_a_sex_dependent_manner:_Contribution_of_prolactin_receptor_to_inflammatory_pain_ L2 - https://journals.physiology.org/doi/10.1152/ajpendo.00187.2013?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -