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Anticancer potential of rhamnocitrin 4'-β-D-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats.
Mol Cell Biochem 2013; 384(1-2):147-53MC

Abstract

The hepatoprotective activity of flavonoid rhamnocitrin 4'-β-D-galactopyranoside (RGP) obtained from leaves of Astragalus hamosus L. against N-diethylnitrosamine (DENA)-induced hepatic cancer in Wistar albino rats was evaluated. Hepatic cancer in rats was induced by single-dose intraperitoneal administration of DENA (200 mg/kg). Induction of hepatic cancer was confirmed after 7 days of DENA administration by measurement of elevated level of serum α-feto protein (AFP). Administration of DENA in a single dose lofted the levels of serum biochemical parameters like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total protein and AFP. Antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and lipid per oxidation (LPO) were annealed significantly by administration of RGP in a dose-dependant manner. The histopathological examination of rat liver section was found to reinforce the biochemical observations significantly. It was observed that a substantial and dose-dependent reversal of DENA-diminished activity of antioxidant enzymes like SOD, CAT, GPx, GST and the reduced DENA-elevated level of LPO with a marked change. Any elevation in the levels of serum markers along with suppression of free radical formation by scavenging the hydroxyl radicals is significantly prevented by RGP. It also modulates the levels of LPO and perceptibly increases the endogenous antioxidant enzymes level in DENA-induced hepatocellular carcinogenesis. The findings suggest that RGP prevents hepatocellular carcinoma by suppressing the marked increase in the levels of serum marker enzymes, and suppresses the free radical by scavenging hydroxyl radicals.

Authors+Show Affiliations

Department of Pharmacology, Luqman College of Pharmacy, Gulbarga, 585204, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24026428

Citation

Saleem, Shakir, et al. "Anticancer Potential of Rhamnocitrin 4'-β-D-galactopyranoside Against N-diethylnitrosamine-induced Hepatocellular Carcinoma in Rats." Molecular and Cellular Biochemistry, vol. 384, no. 1-2, 2013, pp. 147-53.
Saleem S, Shaharyar MA, Khusroo MJ, et al. Anticancer potential of rhamnocitrin 4'-β-D-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats. Mol Cell Biochem. 2013;384(1-2):147-53.
Saleem, S., Shaharyar, M. A., Khusroo, M. J., Ahmad, P., Rahman, R. U., Ahmad, K., ... Imam, F. (2013). Anticancer potential of rhamnocitrin 4'-β-D-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats. Molecular and Cellular Biochemistry, 384(1-2), pp. 147-53. doi:10.1007/s11010-013-1792-6.
Saleem S, et al. Anticancer Potential of Rhamnocitrin 4'-β-D-galactopyranoside Against N-diethylnitrosamine-induced Hepatocellular Carcinoma in Rats. Mol Cell Biochem. 2013;384(1-2):147-53. PubMed PMID: 24026428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anticancer potential of rhamnocitrin 4'-β-D-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats. AU - Saleem,Shakir, AU - Shaharyar,Md Adil, AU - Khusroo,Mohammad Jawed, AU - Ahmad,Parwej, AU - Rahman,Rais Ur, AU - Ahmad,Kamran, AU - Alam,Md Jahangir, AU - Al-Harbi,Naif O, AU - Iqbal,Muzaffar, AU - Imam,Faisal, Y1 - 2013/09/12/ PY - 2013/03/18/received PY - 2013/08/23/accepted PY - 2013/9/13/entrez PY - 2013/9/13/pubmed PY - 2014/10/1/medline SP - 147 EP - 53 JF - Molecular and cellular biochemistry JO - Mol. Cell. Biochem. VL - 384 IS - 1-2 N2 - The hepatoprotective activity of flavonoid rhamnocitrin 4'-β-D-galactopyranoside (RGP) obtained from leaves of Astragalus hamosus L. against N-diethylnitrosamine (DENA)-induced hepatic cancer in Wistar albino rats was evaluated. Hepatic cancer in rats was induced by single-dose intraperitoneal administration of DENA (200 mg/kg). Induction of hepatic cancer was confirmed after 7 days of DENA administration by measurement of elevated level of serum α-feto protein (AFP). Administration of DENA in a single dose lofted the levels of serum biochemical parameters like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total protein and AFP. Antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and lipid per oxidation (LPO) were annealed significantly by administration of RGP in a dose-dependant manner. The histopathological examination of rat liver section was found to reinforce the biochemical observations significantly. It was observed that a substantial and dose-dependent reversal of DENA-diminished activity of antioxidant enzymes like SOD, CAT, GPx, GST and the reduced DENA-elevated level of LPO with a marked change. Any elevation in the levels of serum markers along with suppression of free radical formation by scavenging the hydroxyl radicals is significantly prevented by RGP. It also modulates the levels of LPO and perceptibly increases the endogenous antioxidant enzymes level in DENA-induced hepatocellular carcinogenesis. The findings suggest that RGP prevents hepatocellular carcinoma by suppressing the marked increase in the levels of serum marker enzymes, and suppresses the free radical by scavenging hydroxyl radicals. SN - 1573-4919 UR - https://www.unboundmedicine.com/medline/citation/24026428/Anticancer_potential_of_rhamnocitrin_4'_β_D_galactopyranoside_against_N_diethylnitrosamine_induced_hepatocellular_carcinoma_in_rats_ L2 - https://doi.org/10.1007/s11010-013-1792-6 DB - PRIME DP - Unbound Medicine ER -