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Human astrocytic cells support persistent coxsackievirus B3 infection.
J Virol. 2013 Nov; 87(22):12407-21.JV

Abstract

Enteroviruses can frequently target the human central nervous system to induce a variety of neurological diseases. Although enteroviruses are highly cytolytic, emerging evidence has shown that these viruses can establish persistent infections both in vivo and in vitro. Here, we investigated the susceptibility of three human brain cell lines, CCF-STTG1, T98G, and SK-N-SH, to infection with three enterovirus serotypes: coxsackievirus B3 (CVB3), enterovirus 71, and coxsackievirus A9. Persistent infection was observed in CVB3-infected CCF-STTG1 cells, as evidenced by prolonged detection of infectious virions, viral RNA, and viral antigens. Of note, infected CCF-STTG1 cells expressed the nonfunctional canonical viral receptors coxsackievirus-adenovirus receptor and decay-accelerating factor, while removal of cell surface chondroitin sulfate from CCF-STTG1 cells inhibited the replication of CVB3, suggesting that receptor usage was one of the major limiting factors in CVB3 persistence. In addition, CVB3 curtailed the induction of beta interferon in infected CCF-STTG1 cells, which likely contributed to the initiation of persistence. Furthermore, proinflammatory chemokines and cytokines, such as vascular cell adhesion molecule 1, interleukin-8 (IL-8), and IL-6, were upregulated in CVB3-infected CCF-STTG1 cells and human progenitor-derived astrocytes. Our data together demonstrate the potential of CCF-STTG1 cells to be a novel cell model for studying CVB3-central nervous system interactions, providing the basis toward a better understanding of CVB3-induced chronic neuropathogenesis.

Authors+Show Affiliations

Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24027313

Citation

Zhang, Xiaowei, et al. "Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection." Journal of Virology, vol. 87, no. 22, 2013, pp. 12407-21.
Zhang X, Zheng Z, Shu B, et al. Human astrocytic cells support persistent coxsackievirus B3 infection. J Virol. 2013;87(22):12407-21.
Zhang, X., Zheng, Z., Shu, B., Liu, X., Zhang, Z., Liu, Y., Bai, B., Hu, Q., Mao, P., & Wang, H. (2013). Human astrocytic cells support persistent coxsackievirus B3 infection. Journal of Virology, 87(22), 12407-21. https://doi.org/10.1128/JVI.02090-13
Zhang X, et al. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection. J Virol. 2013;87(22):12407-21. PubMed PMID: 24027313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human astrocytic cells support persistent coxsackievirus B3 infection. AU - Zhang,Xiaowei, AU - Zheng,Zhenhua, AU - Shu,Bo, AU - Liu,Xijuan, AU - Zhang,Zhenfeng, AU - Liu,Yan, AU - Bai,Bingke, AU - Hu,Qinxue, AU - Mao,Panyong, AU - Wang,Hanzhong, Y1 - 2013/09/11/ PY - 2013/9/13/entrez PY - 2013/9/13/pubmed PY - 2014/1/15/medline SP - 12407 EP - 21 JF - Journal of virology JO - J Virol VL - 87 IS - 22 N2 - Enteroviruses can frequently target the human central nervous system to induce a variety of neurological diseases. Although enteroviruses are highly cytolytic, emerging evidence has shown that these viruses can establish persistent infections both in vivo and in vitro. Here, we investigated the susceptibility of three human brain cell lines, CCF-STTG1, T98G, and SK-N-SH, to infection with three enterovirus serotypes: coxsackievirus B3 (CVB3), enterovirus 71, and coxsackievirus A9. Persistent infection was observed in CVB3-infected CCF-STTG1 cells, as evidenced by prolonged detection of infectious virions, viral RNA, and viral antigens. Of note, infected CCF-STTG1 cells expressed the nonfunctional canonical viral receptors coxsackievirus-adenovirus receptor and decay-accelerating factor, while removal of cell surface chondroitin sulfate from CCF-STTG1 cells inhibited the replication of CVB3, suggesting that receptor usage was one of the major limiting factors in CVB3 persistence. In addition, CVB3 curtailed the induction of beta interferon in infected CCF-STTG1 cells, which likely contributed to the initiation of persistence. Furthermore, proinflammatory chemokines and cytokines, such as vascular cell adhesion molecule 1, interleukin-8 (IL-8), and IL-6, were upregulated in CVB3-infected CCF-STTG1 cells and human progenitor-derived astrocytes. Our data together demonstrate the potential of CCF-STTG1 cells to be a novel cell model for studying CVB3-central nervous system interactions, providing the basis toward a better understanding of CVB3-induced chronic neuropathogenesis. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/24027313/Human_astrocytic_cells_support_persistent_coxsackievirus_B3_infection_ L2 - https://journals.asm.org/doi/10.1128/JVI.02090-13?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -