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Cytotoxic effects of Eryngium kotschyi and Eryngium maritimum on Hep2, HepG2, Vero and U138 MG cell lines.
Pharm Biol. 2013 Dec; 51(12):1579-85.PB

Abstract

CONTEXT

Eryngium maritimum L. and the endemic Eryngium kotschyi Boiss. of the Apiaceae family are used for antiinflammatory, antivenom, antinociceptive and diuretic purposes in folk medicine in Turkey.

OBJECTIVE

This study investigated the cytotoxic effects of the plant extracts belonging to Eryngium L. genus on various cell lines.

MATERIALS AND METHODS

Cytotoxic activites of the lyophilized aqueous aereal and root parts of the plant extracts on human hepatocellular carcinoma (HepG2), human laryngeal epidermoid carcinoma (Hep2), human glioma (U138-MG) and African green monkey kidney epithelial (Vero) cell lines at 8.33-266.62 µg/ml concentrations were analyzed by lactate dehydrogenase (LDH) leakage and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) cell viability assays.

RESULTS

Inhibitory concentration 50 (IC50) values were found <100 µg/ml in most cases varying around 16.33-125.66 µg/ml. IC50 values for E. kostchyi and E. maritimum root parts on Hep2 cells (32.86 and 30.25 µg/ml, respectively), E. kotschyi aereal, E. maritimum aereal and root parts on HepG2 cells (31.75, 32.42 and 35.01 µg/ml, respectively) by MTT assay were found to be close to the US National Cancer Institute (NCI) recommendations (IC50 < 30 µg/ml) to define the antivity aganist cancer cells. The lowest IC50 values according to the LDH method were observed in Hep2 cells and the highest in U138-MG cells. Root parts were found to be more toxic than aereal parts for both plants in both methods in general.

DISCUSSION AND CONCLUSION

Both plant extracts exerted cytotoxic activity aganist Hep2 and HepG2 cells, with low IC50 values defining their promising anticancer property according to NCI; however, further analysis are needed to confirm their activity.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine , Ankara , Turkey.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24028780

Citation

Yurdakök, Begüm, and Emine Baydan. "Cytotoxic Effects of Eryngium Kotschyi and Eryngium Maritimum On Hep2, HepG2, Vero and U138 MG Cell Lines." Pharmaceutical Biology, vol. 51, no. 12, 2013, pp. 1579-85.
Yurdakök B, Baydan E. Cytotoxic effects of Eryngium kotschyi and Eryngium maritimum on Hep2, HepG2, Vero and U138 MG cell lines. Pharm Biol. 2013;51(12):1579-85.
Yurdakök, B., & Baydan, E. (2013). Cytotoxic effects of Eryngium kotschyi and Eryngium maritimum on Hep2, HepG2, Vero and U138 MG cell lines. Pharmaceutical Biology, 51(12), 1579-85. https://doi.org/10.3109/13880209.2013.803208
Yurdakök B, Baydan E. Cytotoxic Effects of Eryngium Kotschyi and Eryngium Maritimum On Hep2, HepG2, Vero and U138 MG Cell Lines. Pharm Biol. 2013;51(12):1579-85. PubMed PMID: 24028780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxic effects of Eryngium kotschyi and Eryngium maritimum on Hep2, HepG2, Vero and U138 MG cell lines. AU - Yurdakök,Begüm, AU - Baydan,Emine, Y1 - 2013/09/12/ PY - 2013/9/14/entrez PY - 2013/9/14/pubmed PY - 2014/6/11/medline SP - 1579 EP - 85 JF - Pharmaceutical biology JO - Pharm Biol VL - 51 IS - 12 N2 - CONTEXT: Eryngium maritimum L. and the endemic Eryngium kotschyi Boiss. of the Apiaceae family are used for antiinflammatory, antivenom, antinociceptive and diuretic purposes in folk medicine in Turkey. OBJECTIVE: This study investigated the cytotoxic effects of the plant extracts belonging to Eryngium L. genus on various cell lines. MATERIALS AND METHODS: Cytotoxic activites of the lyophilized aqueous aereal and root parts of the plant extracts on human hepatocellular carcinoma (HepG2), human laryngeal epidermoid carcinoma (Hep2), human glioma (U138-MG) and African green monkey kidney epithelial (Vero) cell lines at 8.33-266.62 µg/ml concentrations were analyzed by lactate dehydrogenase (LDH) leakage and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) cell viability assays. RESULTS: Inhibitory concentration 50 (IC50) values were found <100 µg/ml in most cases varying around 16.33-125.66 µg/ml. IC50 values for E. kostchyi and E. maritimum root parts on Hep2 cells (32.86 and 30.25 µg/ml, respectively), E. kotschyi aereal, E. maritimum aereal and root parts on HepG2 cells (31.75, 32.42 and 35.01 µg/ml, respectively) by MTT assay were found to be close to the US National Cancer Institute (NCI) recommendations (IC50 < 30 µg/ml) to define the antivity aganist cancer cells. The lowest IC50 values according to the LDH method were observed in Hep2 cells and the highest in U138-MG cells. Root parts were found to be more toxic than aereal parts for both plants in both methods in general. DISCUSSION AND CONCLUSION: Both plant extracts exerted cytotoxic activity aganist Hep2 and HepG2 cells, with low IC50 values defining their promising anticancer property according to NCI; however, further analysis are needed to confirm their activity. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/24028780/Cytotoxic_effects_of_Eryngium_kotschyi_and_Eryngium_maritimum_on_Hep2_HepG2_Vero_and_U138_MG_cell_lines_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2013.803208 DB - PRIME DP - Unbound Medicine ER -