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HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply.
New Phytol. 2014 Jan; 201(1):131-143.NP

Abstract

High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tiong J
Australian Centre for Plant Functional Genomics, The University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia.
McDonald GK
School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia.
Genc Y
School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia.
Pedas P
Plant and Soil Science Section, Department of Agriculture and Ecology, University of Copenhagen, Copenhagen, Denmark.
Hayes JE
Australian Centre for Plant Functional Genomics, The University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia.
Toubia J
Australian Centre for Plant Functional Genomics, The University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia.
Langridge P
Australian Centre for Plant Functional Genomics, The University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia.
Huang CY
Australian Centre for Plant Functional Genomics, The University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia.

MeSH

Biological TransportCarrier ProteinsCation Transport ProteinsCell MembraneGene ExpressionGenes, PlantHomeostasisHordeumPlant ProteinsPlant RootsPlant ShootsZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24033183

Citation

Tiong, Jingwen, et al. "HvZIP7 Mediates Zinc Accumulation in Barley (Hordeum Vulgare) at Moderately High Zinc Supply." The New Phytologist, vol. 201, no. 1, 2014, pp. 131-143.
Tiong J, McDonald GK, Genc Y, et al. HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. New Phytol. 2014;201(1):131-143.
Tiong, J., McDonald, G. K., Genc, Y., Pedas, P., Hayes, J. E., Toubia, J., Langridge, P., & Huang, C. Y. (2014). HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. The New Phytologist, 201(1), 131-143. https://doi.org/10.1111/nph.12468
Tiong J, et al. HvZIP7 Mediates Zinc Accumulation in Barley (Hordeum Vulgare) at Moderately High Zinc Supply. New Phytol. 2014;201(1):131-143. PubMed PMID: 24033183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply. AU - Tiong,Jingwen, AU - McDonald,Glenn K, AU - Genc,Yusuf, AU - Pedas,Pai, AU - Hayes,Julie E, AU - Toubia,John, AU - Langridge,Peter, AU - Huang,Chun Y, Y1 - 2013/09/03/ PY - 2013/06/26/received PY - 2013/07/29/accepted PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/7/8/medline KW - Hordeum vulgare (barley) KW - ZIP KW - accumulation KW - low affinity KW - translocation KW - transporter KW - uptake KW - zinc (Zn) SP - 131 EP - 143 JF - The New phytologist JO - New Phytol VL - 201 IS - 1 N2 - High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/24033183/HvZIP7_mediates_zinc_accumulation_in_barley__Hordeum_vulgare__at_moderately_high_zinc_supply_ L2 - https://doi.org/10.1111/nph.12468 DB - PRIME DP - Unbound Medicine ER -