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Revealing in vivo glucose utilization of Gluconobacter oxydans 621H Δmgdh strain by mutagenesis.
Microbiol Res. 2014 May-Jun; 169(5-6):469-75.MR

Abstract

Gluconobacter oxydans, belonging to acetic acid bacteria, is widely used in industrial biotechnology. In our previous study, one of the main glucose metabolic pathways in G. oxydans 621H was blocked by the disruption of the mgdh gene, which is responsible for glucose oxidation to gluconate on cell membrane. The resulting 621H Δmgdh mutant strain showed an enhanced growth and biomass yield on glucose. In order to further understand the intracellular utilization of glucose by 621H Δmgdh, the functions of four fundamental genes, namely glucokinase-encoding glk1 gene, soluble glucose dehydrogenase-encoding sgdh gene, galactose-proton symporter-encoding galp1 and galp2 genes, were investigated. The obtained metabolic characteristics of 621H Δmgdh Δglk1 and 621H Δmgdh Δsgdh double-gene knockout mutants showed that, in vivo, glucose is preferentially phosphorylated to glucose-6-phosphate by glucokinase rather than being oxidized to gluconate by soluble glucose dehydrogenase. In addition, although the galactose-proton symporter-encoding genes were proved to be glucose transporter genes in other organisms, both galp genes (galp 1 and galp2) in G. oxydans were not found to be involved in glucose uptake system, implying that other unknown transporters might be responsible for transporting glucose into the cells.

Authors+Show Affiliations

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China.College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005 Nanning, Guangxi, China.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China. Electronic address: qhua@ecust.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24035043

Citation

Wei, Liujing, et al. "Revealing in Vivo Glucose Utilization of Gluconobacter Oxydans 621H Δmgdh Strain By Mutagenesis." Microbiological Research, vol. 169, no. 5-6, 2014, pp. 469-75.
Wei L, Zhu D, Zhou J, et al. Revealing in vivo glucose utilization of Gluconobacter oxydans 621H Δmgdh strain by mutagenesis. Microbiol Res. 2014;169(5-6):469-75.
Wei, L., Zhu, D., Zhou, J., Zhang, J., Zhu, K., Du, L., & Hua, Q. (2014). Revealing in vivo glucose utilization of Gluconobacter oxydans 621H Δmgdh strain by mutagenesis. Microbiological Research, 169(5-6), 469-75. https://doi.org/10.1016/j.micres.2013.08.002
Wei L, et al. Revealing in Vivo Glucose Utilization of Gluconobacter Oxydans 621H Δmgdh Strain By Mutagenesis. Microbiol Res. 2014 May-Jun;169(5-6):469-75. PubMed PMID: 24035043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Revealing in vivo glucose utilization of Gluconobacter oxydans 621H Δmgdh strain by mutagenesis. AU - Wei,Liujing, AU - Zhu,Danni, AU - Zhou,Jilai, AU - Zhang,Jiajing, AU - Zhu,Kun, AU - Du,Liqin, AU - Hua,Qiang, Y1 - 2013/09/10/ PY - 2013/06/24/received PY - 2013/08/05/revised PY - 2013/08/10/accepted PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/12/15/medline KW - Galactose–proton symporter KW - Glucokinase KW - Gluconobacter oxydans KW - Glucose utilization KW - Soluble glucose dehydrogenase SP - 469 EP - 75 JF - Microbiological research JO - Microbiol. Res. VL - 169 IS - 5-6 N2 - Gluconobacter oxydans, belonging to acetic acid bacteria, is widely used in industrial biotechnology. In our previous study, one of the main glucose metabolic pathways in G. oxydans 621H was blocked by the disruption of the mgdh gene, which is responsible for glucose oxidation to gluconate on cell membrane. The resulting 621H Δmgdh mutant strain showed an enhanced growth and biomass yield on glucose. In order to further understand the intracellular utilization of glucose by 621H Δmgdh, the functions of four fundamental genes, namely glucokinase-encoding glk1 gene, soluble glucose dehydrogenase-encoding sgdh gene, galactose-proton symporter-encoding galp1 and galp2 genes, were investigated. The obtained metabolic characteristics of 621H Δmgdh Δglk1 and 621H Δmgdh Δsgdh double-gene knockout mutants showed that, in vivo, glucose is preferentially phosphorylated to glucose-6-phosphate by glucokinase rather than being oxidized to gluconate by soluble glucose dehydrogenase. In addition, although the galactose-proton symporter-encoding genes were proved to be glucose transporter genes in other organisms, both galp genes (galp 1 and galp2) in G. oxydans were not found to be involved in glucose uptake system, implying that other unknown transporters might be responsible for transporting glucose into the cells. SN - 1618-0623 UR - https://www.unboundmedicine.com/medline/citation/24035043/Revealing_in_vivo_glucose_utilization_of_Gluconobacter_oxydans_621H_Δmgdh_strain_by_mutagenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-5013(13)00127-4 DB - PRIME DP - Unbound Medicine ER -