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The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness.
Comp Biochem Physiol A Mol Integr Physiol. 2013 Dec; 166(4):590-602.CB

Abstract

Satellite cells are multipotential stem cells responsible for muscle growth and regeneration. Satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2 (FGF2) is, in part, regulated by the heparan sulfate proteoglycans syndecan-4 and glypican-1. Syndecan-4 and glypican-1 expression declines with satellite cell age and may be associated with decreased satellite cell activity. The objective of the current study was to determine if overexpression of syndecan-4 and glypican-1 would increase proliferation, differentiation and FGF2 responsiveness in satellite cells isolated from pectoralis major muscle from 16-wk-old turkeys. Overexpression of syndecan-4 and glypican-1 did not have a significant effect on proliferation and differentiation in 1d, 7 wk, and 16 wk satellite cells, and did not affect FGF2 responsiveness during proliferation. Expression of syndecan-4 and glypican-1 increased differentiation at 48 h in 1d, 7 wk, and 16 wk cells treated with FGF2. Expression of myogenic regulatory factors MyoD, myogenin, and MRF4 was affected by the overexpression of syndecan-4 and glypican-1. However, changes in myogenic regulatory factor expression did not have a significant effect on proliferation or differentiation. These data demonstrate that syndecan-4 and glypican-1 are likely not directly associated with the age related decrease in satellite cell activity.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 213 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH 44691, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24036479

Citation

Harthan, Laura B., et al. "The Effect of Syndecan-4 and Glypican-1 Expression On Age-related Changes in Myogenic Satellite Cell Proliferation, Differentiation, and Fibroblast Growth Factor 2 Responsiveness." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 166, no. 4, 2013, pp. 590-602.
Harthan LB, McFarland DC, Velleman SG. The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(4):590-602.
Harthan, L. B., McFarland, D. C., & Velleman, S. G. (2013). The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 166(4), 590-602. https://doi.org/10.1016/j.cbpa.2013.09.007
Harthan LB, McFarland DC, Velleman SG. The Effect of Syndecan-4 and Glypican-1 Expression On Age-related Changes in Myogenic Satellite Cell Proliferation, Differentiation, and Fibroblast Growth Factor 2 Responsiveness. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(4):590-602. PubMed PMID: 24036479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. AU - Harthan,Laura B, AU - McFarland,Douglas C, AU - Velleman,Sandra G, Y1 - 2013/09/12/ PY - 2013/07/19/received PY - 2013/09/07/revised PY - 2013/09/09/accepted PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/5/29/medline KW - 1d, 7wk, and 16wk cells KW - 4′,6-diamidino-2-phenylindole KW - Age KW - BSA KW - CON KW - DAPI KW - DMEM KW - Dulbecco's Modified Eagle's Medium KW - FGF2 KW - Fibroblast growth factor 2 KW - G1 KW - GAPDH KW - GPI KW - Glypican-1 KW - MMLV KW - MRF4 KW - MyoD KW - Myogenin KW - OD KW - PBS KW - PCMS-EGFP empty vector KW - PE KW - PIP(2) KW - PKCα KW - PT KW - Paired box transcription factor 7 KW - Pax7 KW - RBC2 KW - RT KW - RT-qPCR KW - Randombred Control Line 2 turkey KW - Ras homologue gene family, member A KW - RhoA KW - S4 KW - S4G1 KW - Satellite cells KW - Syndecan-4 KW - bovine serum albumin KW - fibroblast growth factor 2 KW - glyceraldehyde-3-phosphate dehydrogenase KW - glycosylphosphatidylinositol KW - glypican-1 KW - moloney murine leukemia virus KW - myogenic differentiation 1 KW - myogenic regulatory factor 4 KW - optical density KW - phosphate buffered saline KW - phosphatidylinositol 4,5-bisphosphate KW - phycoerythrin KW - post-transfection KW - protein kinase C alpha KW - real-time quantitative polymerase chain reaction KW - room temperature KW - satellite cells isolated from turkeys at 1d, 7wk, and 16wk of age, respectively KW - syndecan-4 KW - syndecan-4 and glypican-1 SP - 590 EP - 602 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 166 IS - 4 N2 - Satellite cells are multipotential stem cells responsible for muscle growth and regeneration. Satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2 (FGF2) is, in part, regulated by the heparan sulfate proteoglycans syndecan-4 and glypican-1. Syndecan-4 and glypican-1 expression declines with satellite cell age and may be associated with decreased satellite cell activity. The objective of the current study was to determine if overexpression of syndecan-4 and glypican-1 would increase proliferation, differentiation and FGF2 responsiveness in satellite cells isolated from pectoralis major muscle from 16-wk-old turkeys. Overexpression of syndecan-4 and glypican-1 did not have a significant effect on proliferation and differentiation in 1d, 7 wk, and 16 wk satellite cells, and did not affect FGF2 responsiveness during proliferation. Expression of syndecan-4 and glypican-1 increased differentiation at 48 h in 1d, 7 wk, and 16 wk cells treated with FGF2. Expression of myogenic regulatory factors MyoD, myogenin, and MRF4 was affected by the overexpression of syndecan-4 and glypican-1. However, changes in myogenic regulatory factor expression did not have a significant effect on proliferation or differentiation. These data demonstrate that syndecan-4 and glypican-1 are likely not directly associated with the age related decrease in satellite cell activity. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/24036479/The_effect_of_syndecan_4_and_glypican_1_expression_on_age_related_changes_in_myogenic_satellite_cell_proliferation_differentiation_and_fibroblast_growth_factor_2_responsiveness_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(13)00243-2 DB - PRIME DP - Unbound Medicine ER -