TY - JOUR T1 - The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. AU - Harthan,Laura B, AU - McFarland,Douglas C, AU - Velleman,Sandra G, Y1 - 2013/09/12/ PY - 2013/07/19/received PY - 2013/09/07/revised PY - 2013/09/09/accepted PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/5/29/medline KW - 1d, 7wk, and 16wk cells KW - 4′,6-diamidino-2-phenylindole KW - Age KW - BSA KW - CON KW - DAPI KW - DMEM KW - Dulbecco's Modified Eagle's Medium KW - FGF2 KW - Fibroblast growth factor 2 KW - G1 KW - GAPDH KW - GPI KW - Glypican-1 KW - MMLV KW - MRF4 KW - MyoD KW - Myogenin KW - OD KW - PBS KW - PCMS-EGFP empty vector KW - PE KW - PIP(2) KW - PKCα KW - PT KW - Paired box transcription factor 7 KW - Pax7 KW - RBC2 KW - RT KW - RT-qPCR KW - Randombred Control Line 2 turkey KW - Ras homologue gene family, member A KW - RhoA KW - S4 KW - S4G1 KW - Satellite cells KW - Syndecan-4 KW - bovine serum albumin KW - fibroblast growth factor 2 KW - glyceraldehyde-3-phosphate dehydrogenase KW - glycosylphosphatidylinositol KW - glypican-1 KW - moloney murine leukemia virus KW - myogenic differentiation 1 KW - myogenic regulatory factor 4 KW - optical density KW - phosphate buffered saline KW - phosphatidylinositol 4,5-bisphosphate KW - phycoerythrin KW - post-transfection KW - protein kinase C alpha KW - real-time quantitative polymerase chain reaction KW - room temperature KW - satellite cells isolated from turkeys at 1d, 7wk, and 16wk of age, respectively KW - syndecan-4 KW - syndecan-4 and glypican-1 SP - 590 EP - 602 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 166 IS - 4 N2 - Satellite cells are multipotential stem cells responsible for muscle growth and regeneration. Satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2 (FGF2) is, in part, regulated by the heparan sulfate proteoglycans syndecan-4 and glypican-1. Syndecan-4 and glypican-1 expression declines with satellite cell age and may be associated with decreased satellite cell activity. The objective of the current study was to determine if overexpression of syndecan-4 and glypican-1 would increase proliferation, differentiation and FGF2 responsiveness in satellite cells isolated from pectoralis major muscle from 16-wk-old turkeys. Overexpression of syndecan-4 and glypican-1 did not have a significant effect on proliferation and differentiation in 1d, 7 wk, and 16 wk satellite cells, and did not affect FGF2 responsiveness during proliferation. Expression of syndecan-4 and glypican-1 increased differentiation at 48 h in 1d, 7 wk, and 16 wk cells treated with FGF2. Expression of myogenic regulatory factors MyoD, myogenin, and MRF4 was affected by the overexpression of syndecan-4 and glypican-1. However, changes in myogenic regulatory factor expression did not have a significant effect on proliferation or differentiation. These data demonstrate that syndecan-4 and glypican-1 are likely not directly associated with the age related decrease in satellite cell activity. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/24036479/The_effect_of_syndecan_4_and_glypican_1_expression_on_age_related_changes_in_myogenic_satellite_cell_proliferation_differentiation_and_fibroblast_growth_factor_2_responsiveness_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(13)00243-2 DB - PRIME DP - Unbound Medicine ER -