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Quantitative profiling method for phytohormones and betaines in algae by liquid chromatography electrospray ionization tandem mass spectrometry.
Biomed Chromatogr. 2014 Feb; 28(2):275-80.BC

Abstract

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantitative determination of phytohormones and betaines in algae. The results showed that phytohormones and betaines were separated with high efficiency on Hypersil Gold C18 and Cnwsil SCX columns. Mass spectrometric detection was performed using positive or negative electrospray ionization in selective reaction monitoring mode (SRM). Linearity of the method was good with correlation coefficients (r(2) > 0.9951 in the range of 0.005-5 mg/L. The limits of detection were from 0.004 to 0.86 µg/L and the limits of quantification were in the range from 0.01 to 2.8 µg/L for the investigated phytohormones and betaines. The obtained recoveries varied between 61.33 and 90.39%, and the relative standard deviations were <15%. Using the developed methods, seven types of phytohormones and two types of betaines in Laminaria japonica, and seven types of phytohormones and one type of betaine in Pyropia haitanensis, which were collected in Xiangshan, Zhejiang Province, China, were determined. Thus, LC-MS/MS was demonstrated to be a powerful tool for the comprehensive analysis of phytohormones and betaines in algae, owing to its large dynamic range and excellent sensitivity.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang X
College of Life Science, Taizhou University, Taizhou, 317000, China.
Zhao P
No affiliation info available
Liu X
No affiliation info available
Chen J
No affiliation info available
Xu J
No affiliation info available
Chen H
No affiliation info available
Yan X
No affiliation info available

MeSH

BetaineChromatography, LiquidLaminariaLimit of DetectionLinear ModelsPlant Growth RegulatorsReproducibility of ResultsRhodophytaSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24037856

Citation

Wang, Xiujuan, et al. "Quantitative Profiling Method for Phytohormones and Betaines in Algae By Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry." Biomedical Chromatography : BMC, vol. 28, no. 2, 2014, pp. 275-80.
Wang X, Zhao P, Liu X, et al. Quantitative profiling method for phytohormones and betaines in algae by liquid chromatography electrospray ionization tandem mass spectrometry. Biomed Chromatogr. 2014;28(2):275-80.
Wang, X., Zhao, P., Liu, X., Chen, J., Xu, J., Chen, H., & Yan, X. (2014). Quantitative profiling method for phytohormones and betaines in algae by liquid chromatography electrospray ionization tandem mass spectrometry. Biomedical Chromatography : BMC, 28(2), 275-80. https://doi.org/10.1002/bmc.3018
Wang X, et al. Quantitative Profiling Method for Phytohormones and Betaines in Algae By Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry. Biomed Chromatogr. 2014;28(2):275-80. PubMed PMID: 24037856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative profiling method for phytohormones and betaines in algae by liquid chromatography electrospray ionization tandem mass spectrometry. AU - Wang,Xiujuan, AU - Zhao,Peng, AU - Liu,Xuemei, AU - Chen,Juanjuan, AU - Xu,Jilin, AU - Chen,Haimin, AU - Yan,Xiaojun, Y1 - 2013/09/04/ PY - 2013/05/02/received PY - 2013/07/07/revised PY - 2013/07/12/accepted PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/9/4/medline KW - algae KW - betaines KW - liquid chromatography-tandem mass spectrometry KW - phytohormones KW - quantitative analysis SP - 275 EP - 80 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 28 IS - 2 N2 - A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantitative determination of phytohormones and betaines in algae. The results showed that phytohormones and betaines were separated with high efficiency on Hypersil Gold C18 and Cnwsil SCX columns. Mass spectrometric detection was performed using positive or negative electrospray ionization in selective reaction monitoring mode (SRM). Linearity of the method was good with correlation coefficients (r(2) > 0.9951 in the range of 0.005-5 mg/L. The limits of detection were from 0.004 to 0.86 µg/L and the limits of quantification were in the range from 0.01 to 2.8 µg/L for the investigated phytohormones and betaines. The obtained recoveries varied between 61.33 and 90.39%, and the relative standard deviations were <15%. Using the developed methods, seven types of phytohormones and two types of betaines in Laminaria japonica, and seven types of phytohormones and one type of betaine in Pyropia haitanensis, which were collected in Xiangshan, Zhejiang Province, China, were determined. Thus, LC-MS/MS was demonstrated to be a powerful tool for the comprehensive analysis of phytohormones and betaines in algae, owing to its large dynamic range and excellent sensitivity. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/24037856/Quantitative_profiling_method_for_phytohormones_and_betaines_in_algae_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.3018 DB - PRIME DP - Unbound Medicine ER -
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