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Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin.
J Antimicrob Chemother. 2014 Jan; 69(1):133-5.JA

Abstract

OBJECTIVES

To investigate the reliability of cefoxitin and oxacillin for the detection of mecC-positive Staphylococcus aureus.

METHODS

The susceptibility to cefoxitin and oxacillin of 62 mecC-positive S. aureus isolates was investigated using broth microdilution, agar dilution, Etest and disc diffusion on different types of media. The data were interpreted for the utility of cefoxitin and oxacillin in conjunction with the stated methodologies for the detection of mecC-positive isolates.

RESULTS

Cefoxitin with Mueller-Hinton media from Becton Dickinson and Oxoid detected all mecC-positive isolates when tested by broth microdilution, agar dilution and disc diffusion. By Etest, one isolate was falsely susceptible. Mueller-Hinton agar from bioMérieux was substantially less able to detect these isolates. One isolate was falsely susceptible by agar dilution when using Iso-Sensitest and Columbia agar. Disc diffusion using cefoxitin on Iso-Sensitest agar missed 29% of the isolates. For oxacillin, only agar dilution on Columbia agar + 2% NaCl was able to detect all mecC-positive isolates successfully.

CONCLUSIONS

Cefoxitin used with EUCAST methodology and oxacillin used with agar dilution on Columbia agar + 2% NaCl detected all mecC-positive isolates. These methods with their concomitant agars should be preferred over Iso-Sensitest, which is recommended by the BSAC. It should be noted that for disc diffusion Mueller-Hinton media from bioMérieux performed poorly, with 26%-47% of mecC isolates being falsely susceptible.

Authors+Show Affiliations

Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, Copenhagen 2300, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24038776

Citation

Skov, Robert, et al. "Phenotypic Detection of mecC-MRSA: Cefoxitin Is More Reliable Than Oxacillin." The Journal of Antimicrobial Chemotherapy, vol. 69, no. 1, 2014, pp. 133-5.
Skov R, Larsen AR, Kearns A, et al. Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin. J Antimicrob Chemother. 2014;69(1):133-5.
Skov, R., Larsen, A. R., Kearns, A., Holmes, M., Teale, C., Edwards, G., & Hill, R. (2014). Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin. The Journal of Antimicrobial Chemotherapy, 69(1), 133-5. https://doi.org/10.1093/jac/dkt341
Skov R, et al. Phenotypic Detection of mecC-MRSA: Cefoxitin Is More Reliable Than Oxacillin. J Antimicrob Chemother. 2014;69(1):133-5. PubMed PMID: 24038776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin. AU - Skov,Robert, AU - Larsen,Anders Rhod, AU - Kearns,Angela, AU - Holmes,Mark, AU - Teale,Christopher, AU - Edwards,Giles, AU - Hill,Robert, Y1 - 2013/09/12/ PY - 2013/9/17/entrez PY - 2013/9/17/pubmed PY - 2014/7/26/medline KW - Staphylococcus aureus KW - methicillin resistance KW - susceptibility testing SP - 133 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 69 IS - 1 N2 - OBJECTIVES: To investigate the reliability of cefoxitin and oxacillin for the detection of mecC-positive Staphylococcus aureus. METHODS: The susceptibility to cefoxitin and oxacillin of 62 mecC-positive S. aureus isolates was investigated using broth microdilution, agar dilution, Etest and disc diffusion on different types of media. The data were interpreted for the utility of cefoxitin and oxacillin in conjunction with the stated methodologies for the detection of mecC-positive isolates. RESULTS: Cefoxitin with Mueller-Hinton media from Becton Dickinson and Oxoid detected all mecC-positive isolates when tested by broth microdilution, agar dilution and disc diffusion. By Etest, one isolate was falsely susceptible. Mueller-Hinton agar from bioMérieux was substantially less able to detect these isolates. One isolate was falsely susceptible by agar dilution when using Iso-Sensitest and Columbia agar. Disc diffusion using cefoxitin on Iso-Sensitest agar missed 29% of the isolates. For oxacillin, only agar dilution on Columbia agar + 2% NaCl was able to detect all mecC-positive isolates successfully. CONCLUSIONS: Cefoxitin used with EUCAST methodology and oxacillin used with agar dilution on Columbia agar + 2% NaCl detected all mecC-positive isolates. These methods with their concomitant agars should be preferred over Iso-Sensitest, which is recommended by the BSAC. It should be noted that for disc diffusion Mueller-Hinton media from bioMérieux performed poorly, with 26%-47% of mecC isolates being falsely susceptible. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/24038776/Phenotypic_detection_of_mecC_MRSA:_cefoxitin_is_more_reliable_than_oxacillin_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkt341 DB - PRIME DP - Unbound Medicine ER -