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Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida.
Ecotoxicol Environ Saf. 2013 Dec; 98:219-26.EE

Abstract

The molecular mechanism of silver nanoparticle (AgNP) toxicity, particularly its temporal aspect, is currently limited in the literature. This study seeks to identify and profile changes in molecular response patterns over time during soil exposure of the earthworm Eisenia fetida to AgNPs (82±27 nm) with reference to dissolved silver salt (AgNO₃). Principal component analysis of selected gene and enzyme response profiles revealed dissimilar patterns between AgNO₃ and AgNP treatments and also over time. Despite the observed difference in molecular profiles, the body burdens of total Ag were within the same range (10-40 mg/kg dry weight worm) for both treatments with apparent correlation to the induction pattern of metallothionein. AgNO₃ induced the genes and enzymes related to oxidative stress at day 1, after which markers of energy metabolism were all suppressed at day 2. Exposure to AgNPs likewise led to induction of oxidative stress genes at day 2, but with a temporal pattern shift to immune genes at day 14 following metabolic upregulation at day 7. The involvement of oxidative stress and subsequent alterations in immune gene regulation were as predicted by our in vitro study reported previously, highlighting the importance of immunological endpoints in nanosilver toxicity.

Authors+Show Affiliations

Department of Bioscience-Terrestrial Ecology, Aarhus University, Vejlsøvej 25, DK-8600 Silkeborg, Denmark; iNANO Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24041528

Citation

Hayashi, Yuya, et al. "Time-course Profiling of Molecular Stress Responses to Silver Nanoparticles in the Earthworm Eisenia Fetida." Ecotoxicology and Environmental Safety, vol. 98, 2013, pp. 219-26.
Hayashi Y, Heckmann LH, Simonsen V, et al. Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida. Ecotoxicol Environ Saf. 2013;98:219-26.
Hayashi, Y., Heckmann, L. H., Simonsen, V., & Scott-Fordsmand, J. J. (2013). Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida. Ecotoxicology and Environmental Safety, 98, 219-26. https://doi.org/10.1016/j.ecoenv.2013.08.017
Hayashi Y, et al. Time-course Profiling of Molecular Stress Responses to Silver Nanoparticles in the Earthworm Eisenia Fetida. Ecotoxicol Environ Saf. 2013;98:219-26. PubMed PMID: 24041528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida. AU - Hayashi,Yuya, AU - Heckmann,Lars-Henrik, AU - Simonsen,Vibeke, AU - Scott-Fordsmand,Janeck J, Y1 - 2013/09/13/ PY - 2013/04/05/received PY - 2013/07/22/revised PY - 2013/08/20/accepted PY - 2013/9/18/entrez PY - 2013/9/18/pubmed PY - 2014/3/25/medline KW - Immunity KW - In vivo KW - Metallothionein KW - Oxidative stress KW - Silver nanoparticle KW - Time-course SP - 219 EP - 26 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 98 N2 - The molecular mechanism of silver nanoparticle (AgNP) toxicity, particularly its temporal aspect, is currently limited in the literature. This study seeks to identify and profile changes in molecular response patterns over time during soil exposure of the earthworm Eisenia fetida to AgNPs (82±27 nm) with reference to dissolved silver salt (AgNO₃). Principal component analysis of selected gene and enzyme response profiles revealed dissimilar patterns between AgNO₃ and AgNP treatments and also over time. Despite the observed difference in molecular profiles, the body burdens of total Ag were within the same range (10-40 mg/kg dry weight worm) for both treatments with apparent correlation to the induction pattern of metallothionein. AgNO₃ induced the genes and enzymes related to oxidative stress at day 1, after which markers of energy metabolism were all suppressed at day 2. Exposure to AgNPs likewise led to induction of oxidative stress genes at day 2, but with a temporal pattern shift to immune genes at day 14 following metabolic upregulation at day 7. The involvement of oxidative stress and subsequent alterations in immune gene regulation were as predicted by our in vitro study reported previously, highlighting the importance of immunological endpoints in nanosilver toxicity. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/24041528/Time_course_profiling_of_molecular_stress_responses_to_silver_nanoparticles_in_the_earthworm_Eisenia_fetida_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(13)00361-8 DB - PRIME DP - Unbound Medicine ER -