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7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice.
Mol Med Rep 2013; 8(5):1525-30MM

Abstract

Nuclear factor (NF)-κB expression occurs during sciatic injury. In addition, 7,8-dihydroxycoumarin exhibits a neurotrophic effect on peripheral nerve regeneration. To investigate the effects of 7,8-dihydroxycoumarin on the expression levels of NF-κB in L4-6 spinal cord segments of the injured sciatic nerve in mice and on the functional recovery and regeneration following nerve injury, a total of 160 healthy adult male BALB/c mice underwent unilateral sciatic nerve interruption and anastomosis. The mice were separated into groups and subsequently treated with physiological saline (control) or high, medium or low doses of 7,8-dihydroxycoumarin. NF-κB levels were detected by western blot analysis and quantitative polymerase chain reaction (qPCR), and the sciatic functional index (SFI) was measured. Neuronal apoptosis was detected by terminal-deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) staining. The results revealed that NF-κB was activated in the L4-6 spinal cord connected to the injured sciatic nerve. qPCR and western-blot analysis results showed that the expression levels of NF-κB in the high- and medium-dose groups were significantly lower compared with the low-dose and control groups at 12 h, one day, three days, five days and one week (P<0.05 for each). SFI and TUNEL results demonstrated that the high- and medium-dose groups exhibited improved functional nerve regeneration and reduced apoptosis compared with the low-dose and control groups. In conclusion, 7,8-dihydroxycoumarin is capable of suppressing the immune activation of NF-κB in the neurons of the L4-6 spinal cord connected with the injured sciatic nerve, thereby reducing the focal filtration of inflammatory cells, producing the optimum environment for nerve regeneration.

Authors+Show Affiliations

Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24043116

Citation

Du, Jian-Shi, et al. "7,8-dihydroxycoumarin May Promote Sciatic Nerve Regeneration By Suppressing NF-κB Expression in Mice." Molecular Medicine Reports, vol. 8, no. 5, 2013, pp. 1525-30.
Du JS, Zhao Q, Zhang YL, et al. 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Mol Med Rep. 2013;8(5):1525-30.
Du, J. S., Zhao, Q., Zhang, Y. L., Wang, Y., & Ma, M. (2013). 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Molecular Medicine Reports, 8(5), pp. 1525-30. doi:10.3892/mmr.2013.1682.
Du JS, et al. 7,8-dihydroxycoumarin May Promote Sciatic Nerve Regeneration By Suppressing NF-κB Expression in Mice. Mol Med Rep. 2013;8(5):1525-30. PubMed PMID: 24043116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. AU - Du,Jian-Shi, AU - Zhao,Qing, AU - Zhang,Ying-Li, AU - Wang,Yu, AU - Ma,Ming, Y1 - 2013/09/13/ PY - 2013/03/17/received PY - 2013/09/04/accepted PY - 2013/9/18/entrez PY - 2013/9/18/pubmed PY - 2014/11/14/medline SP - 1525 EP - 30 JF - Molecular medicine reports JO - Mol Med Rep VL - 8 IS - 5 N2 - Nuclear factor (NF)-κB expression occurs during sciatic injury. In addition, 7,8-dihydroxycoumarin exhibits a neurotrophic effect on peripheral nerve regeneration. To investigate the effects of 7,8-dihydroxycoumarin on the expression levels of NF-κB in L4-6 spinal cord segments of the injured sciatic nerve in mice and on the functional recovery and regeneration following nerve injury, a total of 160 healthy adult male BALB/c mice underwent unilateral sciatic nerve interruption and anastomosis. The mice were separated into groups and subsequently treated with physiological saline (control) or high, medium or low doses of 7,8-dihydroxycoumarin. NF-κB levels were detected by western blot analysis and quantitative polymerase chain reaction (qPCR), and the sciatic functional index (SFI) was measured. Neuronal apoptosis was detected by terminal-deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) staining. The results revealed that NF-κB was activated in the L4-6 spinal cord connected to the injured sciatic nerve. qPCR and western-blot analysis results showed that the expression levels of NF-κB in the high- and medium-dose groups were significantly lower compared with the low-dose and control groups at 12 h, one day, three days, five days and one week (P<0.05 for each). SFI and TUNEL results demonstrated that the high- and medium-dose groups exhibited improved functional nerve regeneration and reduced apoptosis compared with the low-dose and control groups. In conclusion, 7,8-dihydroxycoumarin is capable of suppressing the immune activation of NF-κB in the neurons of the L4-6 spinal cord connected with the injured sciatic nerve, thereby reducing the focal filtration of inflammatory cells, producing the optimum environment for nerve regeneration. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/24043116/78_dihydroxycoumarin_may_promote_sciatic_nerve_regeneration_by_suppressing_NF_κB_expression_in_mice_ L2 - http://www.spandidos-publications.com/mmr/8/5/1525 DB - PRIME DP - Unbound Medicine ER -