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[Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye].

Abstract

OBJECTIVE

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of HIV-1.

METHODS

RT-LAMP primers were designed according to conservative sequences of HIV-1 gag gene, and their sensitivity and specificity were evaluated by the established RT-LAMP protocol with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The performance of RT-LAMP on clinical samples was compared with real-time reverse transcription PCR(qRT-PCR).

RESULTS

The RT-LAMP assay showed a high specificity, and its detection limit was 1000 copies RNA per tube. The sensitivity and specificity of this method using 43 clinical samples were 94.6% and 100%, respectively,in comparison with those of qRT-PCR.

CONCLUSION

RT-LAMP assay using hydroxynaphthol blue dye does not need expensive instruments, and offer an alternative for the rapid detection of HIV-1 with the potential to be applied in field diagnosis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

24044219

Citation

Zeng, Ya-Lan, et al. "[Visual Detection of HIV-1 By Reverse Transcription Loop-mediated Isothermal Amplification With the Hydroxynaphthol Blue Dye]." Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, vol. 27, no. 2, 2013, pp. 126-8.
Zeng YL, Zhang XG, Nei K, et al. [Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013;27(2):126-8.
Zeng, Y. L., Zhang, X. G., Nei, K., Zhang, Y., Yang, M. J., Shen, H. W., Wang, J., Shi, L., & Ma, X. J. (2013). [Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, 27(2), 126-8.
Zeng YL, et al. [Visual Detection of HIV-1 By Reverse Transcription Loop-mediated Isothermal Amplification With the Hydroxynaphthol Blue Dye]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013;27(2):126-8. PubMed PMID: 24044219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye]. AU - Zeng,Ya-Lan, AU - Zhang,Xiao-Guang, AU - Nei,Kai, AU - Zhang,Yi, AU - Yang,Meng-Jie, AU - Shen,Hong-Wei, AU - Wang,Ji, AU - Shi,Lei, AU - Ma,Xue-Jun, PY - 2013/9/19/entrez PY - 2013/9/21/pubmed PY - 2013/11/15/medline SP - 126 EP - 8 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 27 IS - 2 N2 - OBJECTIVE: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of HIV-1. METHODS: RT-LAMP primers were designed according to conservative sequences of HIV-1 gag gene, and their sensitivity and specificity were evaluated by the established RT-LAMP protocol with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The performance of RT-LAMP on clinical samples was compared with real-time reverse transcription PCR(qRT-PCR). RESULTS: The RT-LAMP assay showed a high specificity, and its detection limit was 1000 copies RNA per tube. The sensitivity and specificity of this method using 43 clinical samples were 94.6% and 100%, respectively,in comparison with those of qRT-PCR. CONCLUSION: RT-LAMP assay using hydroxynaphthol blue dye does not need expensive instruments, and offer an alternative for the rapid detection of HIV-1 with the potential to be applied in field diagnosis. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/24044219/[Visual_detection_of_HIV_1_by_reverse_transcription_loop_mediated_isothermal_amplification_with_the_hydroxynaphthol_blue_dye]_ L2 - https://www.lens.org/lens/search?q=citation_id:24044219 DB - PRIME DP - Unbound Medicine ER -