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Biological activity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum.
Pharm Biol 2014; 52(2):151-6PB

Abstract

CONTEXT

The search for new sources of natural antioxidants from plant material may have beneficial therapeutic potential for those diseases associated with oxidative stress. The medicinal plant Haplophyllum tuberculatum (Forsskal) A. Juss. (Rutaceae) contains phenolic compounds as main phytochemicals; however, there are no reports on its antioxidant properties.

OBJECTIVE

To evaluate antioxidant and cytoprotective potential of ethanol extract of Haplophyllum tuberculatum aerial parts.

MATERIALS AND METHODS

Total phenol content was determined using Folin-Ciocalteu reagent; antiradical activity was measured using ORAC assay and the analysis of the major polyphenols was carried out using a HPLC-MS method. The antioxidant and cytoprotective effect were also investigated by the MTT assay and DCFH-DA method. The human astrocytoma U373-MG cell line was pretreated with ethanol extract (from 0.025 to 250 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min).

RESULTS AND CONCLUSION

Total phenol content was 46.2 mg gallic acid/g sample and ORAC value was 1.283 µmol TE/mg sample. Chemical constituents were methoxyflavones, flavonols (mainly quercetin derivatives), cinnamic acids and benzoic acids. In cell system model of oxidative stress, pretreatments with ethanol extract at the concentrations of 2.5, 0.25 and 0.025 µg/mL significantly attenuated H2O2-induced loss in viability by 13.5, 17 and 20.5%, respectively. Furthermore, these ethanol extract concentrations markedly inhibited intracellular ROS production with IC50 0.026 µg/mL. These findings demonstrate the beneficial properties of ethanol extract of Haplophyllum tuberculatum aerial parts, rich in phenolic compounds, as antioxidant and radical scavenger ameliorating ROS-related processes and diseases such as several neurodegenerative disorders.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Pharmacy, University Complutense of Madrid , Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24047505

Citation

Eissa, T F., et al. "Biological Activity of HPLC-characterized Ethanol Extract From the Aerial Parts of Haplophyllum Tuberculatum." Pharmaceutical Biology, vol. 52, no. 2, 2014, pp. 151-6.
Eissa TF, González-Burgos E, Carretero ME, et al. Biological activity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum. Pharm Biol. 2014;52(2):151-6.
Eissa, T. F., González-Burgos, E., Carretero, M. E., & Gómez-Serranillos, M. P. (2014). Biological activity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum. Pharmaceutical Biology, 52(2), pp. 151-6. doi:10.3109/13880209.2013.819517.
Eissa TF, et al. Biological Activity of HPLC-characterized Ethanol Extract From the Aerial Parts of Haplophyllum Tuberculatum. Pharm Biol. 2014;52(2):151-6. PubMed PMID: 24047505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological activity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum. AU - Eissa,T F, AU - González-Burgos,E, AU - Carretero,M E, AU - Gómez-Serranillos,M P, Y1 - 2013/09/19/ PY - 2013/9/20/entrez PY - 2013/9/21/pubmed PY - 2014/9/2/medline SP - 151 EP - 6 JF - Pharmaceutical biology JO - Pharm Biol VL - 52 IS - 2 N2 - CONTEXT: The search for new sources of natural antioxidants from plant material may have beneficial therapeutic potential for those diseases associated with oxidative stress. The medicinal plant Haplophyllum tuberculatum (Forsskal) A. Juss. (Rutaceae) contains phenolic compounds as main phytochemicals; however, there are no reports on its antioxidant properties. OBJECTIVE: To evaluate antioxidant and cytoprotective potential of ethanol extract of Haplophyllum tuberculatum aerial parts. MATERIALS AND METHODS: Total phenol content was determined using Folin-Ciocalteu reagent; antiradical activity was measured using ORAC assay and the analysis of the major polyphenols was carried out using a HPLC-MS method. The antioxidant and cytoprotective effect were also investigated by the MTT assay and DCFH-DA method. The human astrocytoma U373-MG cell line was pretreated with ethanol extract (from 0.025 to 250 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min). RESULTS AND CONCLUSION: Total phenol content was 46.2 mg gallic acid/g sample and ORAC value was 1.283 µmol TE/mg sample. Chemical constituents were methoxyflavones, flavonols (mainly quercetin derivatives), cinnamic acids and benzoic acids. In cell system model of oxidative stress, pretreatments with ethanol extract at the concentrations of 2.5, 0.25 and 0.025 µg/mL significantly attenuated H2O2-induced loss in viability by 13.5, 17 and 20.5%, respectively. Furthermore, these ethanol extract concentrations markedly inhibited intracellular ROS production with IC50 0.026 µg/mL. These findings demonstrate the beneficial properties of ethanol extract of Haplophyllum tuberculatum aerial parts, rich in phenolic compounds, as antioxidant and radical scavenger ameliorating ROS-related processes and diseases such as several neurodegenerative disorders. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/24047505/Biological_activity_of_HPLC_characterized_ethanol_extract_from_the_aerial_parts_of_Haplophyllum_tuberculatum_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2013.819517 DB - PRIME DP - Unbound Medicine ER -