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Recent outbreak of aseptic meningitis in Italy due to Echovirus 30 and phylogenetic relationship with other European circulating strains.
J Clin Virol. 2013 Nov; 58(3):579-83.JC

Abstract

BACKGROUND

Enteroviruses (EVs) are common human viral pathogens, causing a variety of diseases, including aseptic meningitis. Recently, EV aseptic meningitis outbreaks have been reported across Europe, but, in Italy, knowledge of recent EV molecular epidemiology is very limited.

OBJECTIVES

We report an outbreak of EV aseptic meningitis in 10 adults in North-Western Italy, from October to November 2012. Patients were parents or close relatives of children <5 years old attending the same class of a nursery school, suffering from a mild febrile upper respiratory disease. Phylogenetic relationship with other European circulating strains was analyzed updating E30 circulation in Italy in recent years.

STUDY DESIGN

EVs were detected from cerebrospinal fluid (CSF) specimens with a real-time reverse transcription polymerase chain reaction and virus isolation was achieved from rectal and pharyngeal swabs. For cluster definition and phylogenetic studies, viral VP1 region was directly amplified and sequenced from CSF.

RESULTS

EVs were identified in CSF from all patients and from rectal and pharyngeal swabs in 7 of them. Direct sequencing of CSF revealed the presence of the same Echovirus 30 (E30) in all patients and phylogenetic analysis identified it as a diverging clade within E30 genotype VII, the most recent strain circulating in UK, Finland and Denmark since 2006.

CONCLUSION

Molecular techniques allowed the rapid identification and typing of E30 from CSF. Phylogenetic analysis revealed that the cluster might be due to a new E30 variant within the genotype VII currently circulating in Europe, thus updating the epidemiology of EV circulation in Italy.

Authors+Show Affiliations

Laboratory of Microbiology and Virology, Infectious Diseases Department, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149 Turin, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24051044

Citation

Milia, Maria Grazia, et al. "Recent Outbreak of Aseptic Meningitis in Italy Due to Echovirus 30 and Phylogenetic Relationship With Other European Circulating Strains." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 58, no. 3, 2013, pp. 579-83.
Milia MG, Cerutti F, Gregori G, et al. Recent outbreak of aseptic meningitis in Italy due to Echovirus 30 and phylogenetic relationship with other European circulating strains. J Clin Virol. 2013;58(3):579-83.
Milia, M. G., Cerutti, F., Gregori, G., Burdino, E., Allice, T., Ruggiero, T., Proia, M., De Rosa, G., Enrico, E., Lipani, F., Di Perri, G., & Ghisetti, V. (2013). Recent outbreak of aseptic meningitis in Italy due to Echovirus 30 and phylogenetic relationship with other European circulating strains. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 58(3), 579-83. https://doi.org/10.1016/j.jcv.2013.08.023
Milia MG, et al. Recent Outbreak of Aseptic Meningitis in Italy Due to Echovirus 30 and Phylogenetic Relationship With Other European Circulating Strains. J Clin Virol. 2013;58(3):579-83. PubMed PMID: 24051044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recent outbreak of aseptic meningitis in Italy due to Echovirus 30 and phylogenetic relationship with other European circulating strains. AU - Milia,Maria Grazia, AU - Cerutti,Francesco, AU - Gregori,Gabriella, AU - Burdino,Elisa, AU - Allice,Tiziano, AU - Ruggiero,Tina, AU - Proia,Maria, AU - De Rosa,Giulia, AU - Enrico,Eugenia, AU - Lipani,Filippo, AU - Di Perri,Giovanni, AU - Ghisetti,Valeria, Y1 - 2013/08/31/ PY - 2013/05/28/received PY - 2013/08/14/revised PY - 2013/08/21/accepted PY - 2013/9/21/entrez PY - 2013/9/21/pubmed PY - 2014/6/3/medline KW - Aseptic meningitis KW - CSF KW - E30 KW - EV KW - Echovirus 30 KW - Enterovirus KW - Outbreak KW - PCR KW - cerebrospinal fluid KW - enterovirus KW - polymerase chain reaction SP - 579 EP - 83 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 58 IS - 3 N2 - BACKGROUND: Enteroviruses (EVs) are common human viral pathogens, causing a variety of diseases, including aseptic meningitis. Recently, EV aseptic meningitis outbreaks have been reported across Europe, but, in Italy, knowledge of recent EV molecular epidemiology is very limited. OBJECTIVES: We report an outbreak of EV aseptic meningitis in 10 adults in North-Western Italy, from October to November 2012. Patients were parents or close relatives of children <5 years old attending the same class of a nursery school, suffering from a mild febrile upper respiratory disease. Phylogenetic relationship with other European circulating strains was analyzed updating E30 circulation in Italy in recent years. STUDY DESIGN: EVs were detected from cerebrospinal fluid (CSF) specimens with a real-time reverse transcription polymerase chain reaction and virus isolation was achieved from rectal and pharyngeal swabs. For cluster definition and phylogenetic studies, viral VP1 region was directly amplified and sequenced from CSF. RESULTS: EVs were identified in CSF from all patients and from rectal and pharyngeal swabs in 7 of them. Direct sequencing of CSF revealed the presence of the same Echovirus 30 (E30) in all patients and phylogenetic analysis identified it as a diverging clade within E30 genotype VII, the most recent strain circulating in UK, Finland and Denmark since 2006. CONCLUSION: Molecular techniques allowed the rapid identification and typing of E30 from CSF. Phylogenetic analysis revealed that the cluster might be due to a new E30 variant within the genotype VII currently circulating in Europe, thus updating the epidemiology of EV circulation in Italy. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/24051044/Recent_outbreak_of_aseptic_meningitis_in_Italy_due_to_Echovirus_30_and_phylogenetic_relationship_with_other_European_circulating_strains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(13)00347-8 DB - PRIME DP - Unbound Medicine ER -