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A long acting biodegradable controlled delivery of chitosan microspheres loaded with tetanus toxoide as model antigen.
Biomed Pharmacother. 2014 Mar; 68(2):225-30.BP

Abstract

The chitosan microspheres formulated by emulsion cross-linking method were found to be smooth and spherical without aggregation. The particle size range was between 1 and 90μm. The particle sizes were found to be influenced by the concentration of the chitosan gel. Tetanus toxoide (TT) vaccine was loaded by passive adsorption from an aqueous solution into the preformed chitosan microspheres cross-linked with glutaraldehyde. The loaded TT on to microspheres was estimated by ELISA method. The loading capacity was found to be 40% with microspheres prepared with 1% chitosan gel, 43% for 2% and 46% for the mixed batch of microspheres prepared from 1% and 2% chitosan gel. The loading efficiency was found to decrease with increase in the concentration of chitosan gel. The in vitro release of the antigenic TT into the phosphate buffer at 37°C from different batch of microspheres was studied and release had a remarkable dependence on the size of micropsheres. The percentage release of TT from chitosan microspheres prepared from 1% chitosan gel was 2.7% in 120days and that from 2% chitosan gel was only 2%. The mixed batch of microspheres could release 2.3% in 120days. The antigen integrity was investigated by SDS-PAGE with brilliant blue staining. The SDS-PAGE analysis confirmed that the antigen integrity was not affected by passive adsorption of protein antigen to preformed chitosan microspheres. The study revealed that the cross-linked chitosan microspheres would be an interesting system for long-term delivery of macromolecules drugs.

Authors+Show Affiliations

National College of Pharmacy, Manassery, Kozhikode 673602, Kerala State, India. Electronic address: sujithvarma@yahoo.com.Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad P.O, Kannur District, 670661, Kerala State, India. Electronic address: c_sadasivan@rediffmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24051124

Citation

Varma, Sujith, and C Sadasivan. "A Long Acting Biodegradable Controlled Delivery of Chitosan Microspheres Loaded With Tetanus Toxoide as Model Antigen." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 68, no. 2, 2014, pp. 225-30.
Varma S, Sadasivan C. A long acting biodegradable controlled delivery of chitosan microspheres loaded with tetanus toxoide as model antigen. Biomed Pharmacother. 2014;68(2):225-30.
Varma, S., & Sadasivan, C. (2014). A long acting biodegradable controlled delivery of chitosan microspheres loaded with tetanus toxoide as model antigen. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 68(2), 225-30. https://doi.org/10.1016/j.biopha.2013.08.009
Varma S, Sadasivan C. A Long Acting Biodegradable Controlled Delivery of Chitosan Microspheres Loaded With Tetanus Toxoide as Model Antigen. Biomed Pharmacother. 2014;68(2):225-30. PubMed PMID: 24051124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A long acting biodegradable controlled delivery of chitosan microspheres loaded with tetanus toxoide as model antigen. AU - Varma,Sujith, AU - Sadasivan,C, Y1 - 2013/09/03/ PY - 2013/07/16/received PY - 2013/08/09/accepted PY - 2013/9/21/entrez PY - 2013/9/21/pubmed PY - 2014/11/18/medline KW - Chitosan microspheres KW - Tetanus toxoide KW - Vaccines SP - 225 EP - 30 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 68 IS - 2 N2 - The chitosan microspheres formulated by emulsion cross-linking method were found to be smooth and spherical without aggregation. The particle size range was between 1 and 90μm. The particle sizes were found to be influenced by the concentration of the chitosan gel. Tetanus toxoide (TT) vaccine was loaded by passive adsorption from an aqueous solution into the preformed chitosan microspheres cross-linked with glutaraldehyde. The loaded TT on to microspheres was estimated by ELISA method. The loading capacity was found to be 40% with microspheres prepared with 1% chitosan gel, 43% for 2% and 46% for the mixed batch of microspheres prepared from 1% and 2% chitosan gel. The loading efficiency was found to decrease with increase in the concentration of chitosan gel. The in vitro release of the antigenic TT into the phosphate buffer at 37°C from different batch of microspheres was studied and release had a remarkable dependence on the size of micropsheres. The percentage release of TT from chitosan microspheres prepared from 1% chitosan gel was 2.7% in 120days and that from 2% chitosan gel was only 2%. The mixed batch of microspheres could release 2.3% in 120days. The antigen integrity was investigated by SDS-PAGE with brilliant blue staining. The SDS-PAGE analysis confirmed that the antigen integrity was not affected by passive adsorption of protein antigen to preformed chitosan microspheres. The study revealed that the cross-linked chitosan microspheres would be an interesting system for long-term delivery of macromolecules drugs. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/24051124/A_long_acting_biodegradable_controlled_delivery_of_chitosan_microspheres_loaded_with_tetanus_toxoide_as_model_antigen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(13)00098-X DB - PRIME DP - Unbound Medicine ER -