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The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves.
J Biol Chem. 2013 Nov 01; 288(44):31701-14.JB

Abstract

Jasmonates (JAs) are a class of signaling compounds that mediate complex developmental and adaptative responses in plants. JAs derive from jasmonic acid (JA) through various enzymatic modifications, including conjugation to amino acids or oxidation, yielding an array of derivatives. The main hormonal signal, jasmonoyl-L-isoleucine (JA-Ile), has been found recently to undergo catabolic inactivation by cytochrome P450-mediated oxidation. We characterize here two amidohydrolases, IAR3 and ILL6, that define a second pathway for JA-Ile turnover during the wound response in Arabidopsis leaves. Biochemical and genetic evidence indicates that these two enzymes cleave the JA-Ile signal, but act also on the 12OH-JA-Ile conjugate. We also show that unexpectedly, the abundant accumulation of tuberonic acid (12OH-JA) after wounding originates partly through a sequential pathway involving (i) conjugation of JA to Ile, (ii) oxidation of the JA-Ile conjugate, and (iii) cleavage under the action of the amidohydrolases. The coordinated actions of oxidative and hydrolytic branches in the jasmonate pathway highlight novel mechanisms of JA-Ile hormone turnover and redefine the dynamic metabolic grid of jasmonate conversion in the wound response.

Authors+Show Affiliations

From the Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24052260

Citation

Widemann, Emilie, et al. "The Amidohydrolases IAR3 and ILL6 Contribute to Jasmonoyl-isoleucine Hormone Turnover and Generate 12-hydroxyjasmonic Acid Upon Wounding in Arabidopsis Leaves." The Journal of Biological Chemistry, vol. 288, no. 44, 2013, pp. 31701-14.
Widemann E, Miesch L, Lugan R, et al. The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves. J Biol Chem. 2013;288(44):31701-14.
Widemann, E., Miesch, L., Lugan, R., Holder, E., Heinrich, C., Aubert, Y., Miesch, M., Pinot, F., & Heitz, T. (2013). The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves. The Journal of Biological Chemistry, 288(44), 31701-14. https://doi.org/10.1074/jbc.M113.499228
Widemann E, et al. The Amidohydrolases IAR3 and ILL6 Contribute to Jasmonoyl-isoleucine Hormone Turnover and Generate 12-hydroxyjasmonic Acid Upon Wounding in Arabidopsis Leaves. J Biol Chem. 2013 Nov 1;288(44):31701-14. PubMed PMID: 24052260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves. AU - Widemann,Emilie, AU - Miesch,Laurence, AU - Lugan,Raphaël, AU - Holder,Emilie, AU - Heinrich,Clément, AU - Aubert,Yann, AU - Miesch,Michel, AU - Pinot,Franck, AU - Heitz,Thierry, Y1 - 2013/09/19/ PY - 2013/9/21/entrez PY - 2013/9/21/pubmed PY - 2014/1/1/medline KW - Arabidopsis KW - Conjugate KW - Enzymes KW - Hormone Catabolism KW - Hydrolases KW - Jasmonate Metabolism KW - Phytohormones KW - Plant Defense KW - Wound Response SP - 31701 EP - 14 JF - The Journal of biological chemistry JO - J Biol Chem VL - 288 IS - 44 N2 - Jasmonates (JAs) are a class of signaling compounds that mediate complex developmental and adaptative responses in plants. JAs derive from jasmonic acid (JA) through various enzymatic modifications, including conjugation to amino acids or oxidation, yielding an array of derivatives. The main hormonal signal, jasmonoyl-L-isoleucine (JA-Ile), has been found recently to undergo catabolic inactivation by cytochrome P450-mediated oxidation. We characterize here two amidohydrolases, IAR3 and ILL6, that define a second pathway for JA-Ile turnover during the wound response in Arabidopsis leaves. Biochemical and genetic evidence indicates that these two enzymes cleave the JA-Ile signal, but act also on the 12OH-JA-Ile conjugate. We also show that unexpectedly, the abundant accumulation of tuberonic acid (12OH-JA) after wounding originates partly through a sequential pathway involving (i) conjugation of JA to Ile, (ii) oxidation of the JA-Ile conjugate, and (iii) cleavage under the action of the amidohydrolases. The coordinated actions of oxidative and hydrolytic branches in the jasmonate pathway highlight novel mechanisms of JA-Ile hormone turnover and redefine the dynamic metabolic grid of jasmonate conversion in the wound response. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/24052260/The_amidohydrolases_IAR3_and_ILL6_contribute_to_jasmonoyl_isoleucine_hormone_turnover_and_generate_12_hydroxyjasmonic_acid_upon_wounding_in_Arabidopsis_leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/M113.499228 DB - PRIME DP - Unbound Medicine ER -