Tags

Type your tag names separated by a space and hit enter

Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen.
BMC Infect Dis. 2013 Sep 20; 13:438.BI

Abstract

BACKGROUND

Leptospirosis is a potentially lethal zoonosis mainly affecting low-resource tropical countries, including Peru and its neighbouring countries. Timely diagnosis of leptospirosis is critical but may be challenging in the regions where it is most prevalent. The serodiagnostic gold standard microagglutination test (MAT) may be technically prohibitive. Our objective in this study was to assess the sensitivity, specificity, and predictive value of an IgM antibody capture enzyme-linked immunoassay (MAC-ELISA) derived from the M20 strain of Leptospira interrogans serovar Copenhageni (M20) by comparison to MAT, which was used as the gold standard method of diagnosis.

METHODS

Acute and convalescent sera from participants participating in a passive febrile surveillance study in multiple regions of Peru were tested by both IgM MAC-ELISA and MAT. The sensitivity, specificity, positive and negative predictive value (PPV, NPV) of the MAC-ELISA assay for acute, convalescent and paired sera by comparison to MAT were calculated.

RESULTS

The sensitivity, specificity, PPV and NPV of the MAC-ELISA assay for acute sera were 92.3%, 56.0%, 35.3% and 96.6% respectively. For convalescent sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.3%, 51.5%, 63.6% and 89.5% respectively. For paired sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.6%, 37.5%, 59.2%, 85.7% respectively.

CONCLUSIONS

The M20 MAC-ELISA assay performed with a high sensitivity and low specificity in the acute phase of illness. Sensitivity was similar as compared with MAT in the convalescent phase and specificity remained low. Paired sera were the most sensitive but least specific by comparison to MAT serodiagnosis. NPV for acute, convalescent and paired sera was high. The limited specificity and high sensitivity of the MAC-ELISA IgM suggests that it would be most valuable to exclude leptospirosis in low-resource regions that lack immediate access to definitive reference laboratory techniques such as MAT.

Authors+Show Affiliations

U,S, Naval Medical Research Unit No, 6, Lima, Peru. spollett@med.usyd.edu.au.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24053555

Citation

Canal, Enrique, et al. "Detection of Human Leptospirosis as a Cause of Acute Fever By Capture ELISA Using a Leptospira Interrogans Serovar Copenhageni (M20) Derived Antigen." BMC Infectious Diseases, vol. 13, 2013, p. 438.
Canal E, Pollett S, Heitzinger K, et al. Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen. BMC Infect Dis. 2013;13:438.
Canal, E., Pollett, S., Heitzinger, K., Gregory, M., Kasper, M., Halsey, E., Meza, Y., Campos, K., Perez, J., Meza, R., Bernal, M., Guillen, A., Kochel, T. J., Espinosa, B., Hall, E. R., & Maves, R. C. (2013). Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen. BMC Infectious Diseases, 13, 438. https://doi.org/10.1186/1471-2334-13-438
Canal E, et al. Detection of Human Leptospirosis as a Cause of Acute Fever By Capture ELISA Using a Leptospira Interrogans Serovar Copenhageni (M20) Derived Antigen. BMC Infect Dis. 2013 Sep 20;13:438. PubMed PMID: 24053555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen. AU - Canal,Enrique, AU - Pollett,Simon, AU - Heitzinger,Kristen, AU - Gregory,Michael, AU - Kasper,Matthew, AU - Halsey,Eric, AU - Meza,Yocelinda, AU - Campos,Kalina, AU - Perez,Juan, AU - Meza,Rina, AU - Bernal,Maruja, AU - Guillen,Alfredo, AU - Kochel,Tadeusz J, AU - Espinosa,Benjamin, AU - Hall,Eric R, AU - Maves,Ryan C, Y1 - 2013/09/20/ PY - 2013/04/06/received PY - 2013/08/27/accepted PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/6/24/medline SP - 438 EP - 438 JF - BMC infectious diseases JO - BMC Infect Dis VL - 13 N2 - BACKGROUND: Leptospirosis is a potentially lethal zoonosis mainly affecting low-resource tropical countries, including Peru and its neighbouring countries. Timely diagnosis of leptospirosis is critical but may be challenging in the regions where it is most prevalent. The serodiagnostic gold standard microagglutination test (MAT) may be technically prohibitive. Our objective in this study was to assess the sensitivity, specificity, and predictive value of an IgM antibody capture enzyme-linked immunoassay (MAC-ELISA) derived from the M20 strain of Leptospira interrogans serovar Copenhageni (M20) by comparison to MAT, which was used as the gold standard method of diagnosis. METHODS: Acute and convalescent sera from participants participating in a passive febrile surveillance study in multiple regions of Peru were tested by both IgM MAC-ELISA and MAT. The sensitivity, specificity, positive and negative predictive value (PPV, NPV) of the MAC-ELISA assay for acute, convalescent and paired sera by comparison to MAT were calculated. RESULTS: The sensitivity, specificity, PPV and NPV of the MAC-ELISA assay for acute sera were 92.3%, 56.0%, 35.3% and 96.6% respectively. For convalescent sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.3%, 51.5%, 63.6% and 89.5% respectively. For paired sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.6%, 37.5%, 59.2%, 85.7% respectively. CONCLUSIONS: The M20 MAC-ELISA assay performed with a high sensitivity and low specificity in the acute phase of illness. Sensitivity was similar as compared with MAT in the convalescent phase and specificity remained low. Paired sera were the most sensitive but least specific by comparison to MAT serodiagnosis. NPV for acute, convalescent and paired sera was high. The limited specificity and high sensitivity of the MAC-ELISA IgM suggests that it would be most valuable to exclude leptospirosis in low-resource regions that lack immediate access to definitive reference laboratory techniques such as MAT. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/24053555/Detection_of_human_leptospirosis_as_a_cause_of_acute_fever_by_capture_ELISA_using_a_Leptospira_interrogans_serovar_Copenhageni__M20__derived_antigen_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-13-438 DB - PRIME DP - Unbound Medicine ER -