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Simultaneous analysis of acrylamide and its key precursors, intermediates, and products in model systems by liquid chromatography-triple quadrupole mass spectrometry.
Anal Chem. 2013 Oct 01; 85(19):9262-71.AC

Abstract

A new analytical setup allowing the simultaneous analysis of precursors, intermediates and products in Maillard reaction model systems was developed to investigate the formation and mitigation kinetics of acrylamide. It was based on high-performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (HPLC-MS/MS). Chromatography and mass spectrometry conditions were optimized to permit simultaneous monitoring of compounds relevant to acrylamide, such as asparagine, glucose, glycine, N-(1-deoxy-D-fructos-1-yl)-glycine (DFG), N-(1-deoxy-D-fructos-1-yl)-asparagine (DFA), and four reaction products between acrylamide and glycine. A fairly good separation was achieved on a Venusil MP-C18 column using an isocratic eluent of 0.5% formic acid in water at a flow rate of 0.4 mL/min. Triple quadrupole mass spectrometry in multiple reaction monitoring mode (MRM) was applied to obtain good sensitivity. All of the ten key reaction compounds were separated and determined in a single run within 15 min. High correlation coefficients (r > 0.99) of the ten analytes were obtained in their respective linear ranges. The method was capable to accurately quantify the ten key compounds in model systems during heating. The results showed that acrylamide formed in Maillard reaction could be reduced by reacting directly with glycine, and acrylamide concentration constantly decreased by about 60% within the 180 min heating at 150 °C when glycine was equal to asparagine and glucose in molecular concentration in model systems. This method helps achieve reduction in both time and labor of analysis of a large number of samples.

Authors+Show Affiliations

College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University , Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24053692

Citation

Liu, Jie, et al. "Simultaneous Analysis of Acrylamide and Its Key Precursors, Intermediates, and Products in Model Systems By Liquid Chromatography-triple Quadrupole Mass Spectrometry." Analytical Chemistry, vol. 85, no. 19, 2013, pp. 9262-71.
Liu J, Man Y, Zhu Y, et al. Simultaneous analysis of acrylamide and its key precursors, intermediates, and products in model systems by liquid chromatography-triple quadrupole mass spectrometry. Anal Chem. 2013;85(19):9262-71.
Liu, J., Man, Y., Zhu, Y., Hu, X., & Chen, F. (2013). Simultaneous analysis of acrylamide and its key precursors, intermediates, and products in model systems by liquid chromatography-triple quadrupole mass spectrometry. Analytical Chemistry, 85(19), 9262-71. https://doi.org/10.1021/ac4019928
Liu J, et al. Simultaneous Analysis of Acrylamide and Its Key Precursors, Intermediates, and Products in Model Systems By Liquid Chromatography-triple Quadrupole Mass Spectrometry. Anal Chem. 2013 Oct 1;85(19):9262-71. PubMed PMID: 24053692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous analysis of acrylamide and its key precursors, intermediates, and products in model systems by liquid chromatography-triple quadrupole mass spectrometry. AU - Liu,Jie, AU - Man,Yong, AU - Zhu,Yuchen, AU - Hu,Xiaosong, AU - Chen,Fang, Y1 - 2013/09/20/ PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/4/17/medline SP - 9262 EP - 71 JF - Analytical chemistry JO - Anal Chem VL - 85 IS - 19 N2 - A new analytical setup allowing the simultaneous analysis of precursors, intermediates and products in Maillard reaction model systems was developed to investigate the formation and mitigation kinetics of acrylamide. It was based on high-performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (HPLC-MS/MS). Chromatography and mass spectrometry conditions were optimized to permit simultaneous monitoring of compounds relevant to acrylamide, such as asparagine, glucose, glycine, N-(1-deoxy-D-fructos-1-yl)-glycine (DFG), N-(1-deoxy-D-fructos-1-yl)-asparagine (DFA), and four reaction products between acrylamide and glycine. A fairly good separation was achieved on a Venusil MP-C18 column using an isocratic eluent of 0.5% formic acid in water at a flow rate of 0.4 mL/min. Triple quadrupole mass spectrometry in multiple reaction monitoring mode (MRM) was applied to obtain good sensitivity. All of the ten key reaction compounds were separated and determined in a single run within 15 min. High correlation coefficients (r > 0.99) of the ten analytes were obtained in their respective linear ranges. The method was capable to accurately quantify the ten key compounds in model systems during heating. The results showed that acrylamide formed in Maillard reaction could be reduced by reacting directly with glycine, and acrylamide concentration constantly decreased by about 60% within the 180 min heating at 150 °C when glycine was equal to asparagine and glucose in molecular concentration in model systems. This method helps achieve reduction in both time and labor of analysis of a large number of samples. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/24053692/Simultaneous_analysis_of_acrylamide_and_its_key_precursors_intermediates_and_products_in_model_systems_by_liquid_chromatography_triple_quadrupole_mass_spectrometry_ L2 - https://doi.org/10.1021/ac4019928 DB - PRIME DP - Unbound Medicine ER -