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Molecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil.
J Dairy Sci. 2013; 96(11):6856-6862.JD

Abstract

Mastitis is an important disease for the dairy industry worldwide, causing economic losses and reducing milk quality and production. Staphylococcus aureus is a worldwide agent of this intramammary infection, which also causes foodborne diseases. The objective of this study was to determine the frequency of methicillin-susceptible Staphylococcus aureus (MSSA) isolates in milk of mastitis cows in Brazil and to analyze the genetic lineages and the content of antimicrobial resistance genes and virulence factors among these isolates. Fifty-six MSSA isolates were recovered from 1,484 milk samples (positive for the California mastitis test) of 518 cows from 11 different farms in Brazil (representing 51% of total Staph. aureus obtained), and they were further characterized. Methicillin-susceptible Staphylococcus aureus were isolated from 3.7% of California mastitis test-positive tested milk samples and from 6.2% of tested mastitic cows. Methicillin-susceptible Staphylococcus aureus isolates were characterized by spa typing, agr typing, and multilocus sequence typing, and resistance and virulence traits were investigated by PCR. Seven spa types were identified among MSSA (% of isolates): t127 (44.6), t605 (37.5), t002, t1784, t2066 (1.8), and 2 new ones: t10856 (10.7) and t10852 (1.8). Five distinct sequence types (ST) were detected (% of isolates): ST1 (46.4), ST126 (37.5), ST133 (10.7), ST5 (3.6), and a novel ST registered as ST2493 (1.8). Resistances were detected for streptomycin, chloramphenicol, and tetracycline. One strain contained the chloramphenicol resistance gene (fexA; included within transposon Tn558) and 3 strains contained the tetracycline resistance gene [tet(K)]. Methicillin-susceptible Staphylococcus aureus strains were susceptible to most of the antibiotics studied and lacked the virulence genes of Panton-Valentine leukocidin (lukF/S-PV), toxic shock syndrome toxin 1 (tst), exfoliative toxin A (eta), and exfoliative toxin B (etb), as well as the genes of the immune evasion cluster. Methicillin-susceptible Staphylococcus aureus isolates were detected in a relatively low proportion of cows with mastitis (6.2%) and recovered isolates presented high diversity of genetic lineages, with CC1 and CC126 the predominant clonal complexes, and CC133 also being detected. Larger epidemiological studies with molecular characterization of isolates are required to deepen the knowledge on the circulating genetic lineages among the cow population with mastitis.

Authors+Show Affiliations

Department of Microbiology and Immunology, SP, Brazil.Department of Hygiene Veterinary and Public Health, Universidade Estadual Paulista (UNESP), Botucatu 18.618-970, SP, Brazil.Department of Hygiene Veterinary and Public Health, Universidade Estadual Paulista (UNESP), Botucatu 18.618-970, SP, Brazil.Department of Microbiology and Immunology, SP, Brazil.Department of Food and Agriculture, University of La Rioja, 26006 Logroño, Spain.Department of Food and Agriculture, University of La Rioja, 26006 Logroño, Spain.Department of Hygiene Veterinary and Public Health, Universidade Estadual Paulista (UNESP), Botucatu 18.618-970, SP, Brazil.Department of Microbiology and Immunology, SP, Brazil.Department of Food and Agriculture, University of La Rioja, 26006 Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24054305

Citation

Silva, N C C., et al. "Molecular Characterization and Clonal Diversity of Methicillin-susceptible Staphylococcus Aureus in Milk of Cows With Mastitis in Brazil." Journal of Dairy Science, vol. 96, no. 11, 2013, pp. 6856-6862.
Silva NCC, Guimarães FF, Manzi MP, et al. Molecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil. J Dairy Sci. 2013;96(11):6856-6862.
Silva, N. C. C., Guimarães, F. F., Manzi, M. P., Budri, P. E., Gómez-Sanz, E., Benito, D., Langoni, H., Rall, V. L. M., & Torres, C. (2013). Molecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil. Journal of Dairy Science, 96(11), 6856-6862. https://doi.org/10.3168/jds.2013-6719
Silva NCC, et al. Molecular Characterization and Clonal Diversity of Methicillin-susceptible Staphylococcus Aureus in Milk of Cows With Mastitis in Brazil. J Dairy Sci. 2013;96(11):6856-6862. PubMed PMID: 24054305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil. AU - Silva,N C C, AU - Guimarães,F F, AU - Manzi,M P, AU - Budri,P E, AU - Gómez-Sanz,E, AU - Benito,D, AU - Langoni,H, AU - Rall,V L M, AU - Torres,C, Y1 - 2013/09/18/ PY - 2013/02/21/received PY - 2013/07/22/accepted PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2015/6/16/medline KW - Staphylococcus aureus KW - mastitis KW - methicillin susceptibility KW - spa typing SP - 6856 EP - 6862 JF - Journal of dairy science JO - J Dairy Sci VL - 96 IS - 11 N2 - Mastitis is an important disease for the dairy industry worldwide, causing economic losses and reducing milk quality and production. Staphylococcus aureus is a worldwide agent of this intramammary infection, which also causes foodborne diseases. The objective of this study was to determine the frequency of methicillin-susceptible Staphylococcus aureus (MSSA) isolates in milk of mastitis cows in Brazil and to analyze the genetic lineages and the content of antimicrobial resistance genes and virulence factors among these isolates. Fifty-six MSSA isolates were recovered from 1,484 milk samples (positive for the California mastitis test) of 518 cows from 11 different farms in Brazil (representing 51% of total Staph. aureus obtained), and they were further characterized. Methicillin-susceptible Staphylococcus aureus were isolated from 3.7% of California mastitis test-positive tested milk samples and from 6.2% of tested mastitic cows. Methicillin-susceptible Staphylococcus aureus isolates were characterized by spa typing, agr typing, and multilocus sequence typing, and resistance and virulence traits were investigated by PCR. Seven spa types were identified among MSSA (% of isolates): t127 (44.6), t605 (37.5), t002, t1784, t2066 (1.8), and 2 new ones: t10856 (10.7) and t10852 (1.8). Five distinct sequence types (ST) were detected (% of isolates): ST1 (46.4), ST126 (37.5), ST133 (10.7), ST5 (3.6), and a novel ST registered as ST2493 (1.8). Resistances were detected for streptomycin, chloramphenicol, and tetracycline. One strain contained the chloramphenicol resistance gene (fexA; included within transposon Tn558) and 3 strains contained the tetracycline resistance gene [tet(K)]. Methicillin-susceptible Staphylococcus aureus strains were susceptible to most of the antibiotics studied and lacked the virulence genes of Panton-Valentine leukocidin (lukF/S-PV), toxic shock syndrome toxin 1 (tst), exfoliative toxin A (eta), and exfoliative toxin B (etb), as well as the genes of the immune evasion cluster. Methicillin-susceptible Staphylococcus aureus isolates were detected in a relatively low proportion of cows with mastitis (6.2%) and recovered isolates presented high diversity of genetic lineages, with CC1 and CC126 the predominant clonal complexes, and CC133 also being detected. Larger epidemiological studies with molecular characterization of isolates are required to deepen the knowledge on the circulating genetic lineages among the cow population with mastitis. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/24054305/Molecular_characterization_and_clonal_diversity_of_methicillin_susceptible_Staphylococcus_aureus_in_milk_of_cows_with_mastitis_in_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(13)00641-3 DB - PRIME DP - Unbound Medicine ER -