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Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification.
Talanta. 2013 Oct 15; 115:761-7.T

Abstract

The accidental contamination of Salmonella in raw and processed foods is a major problem for the food industry worldwide. At present many of the currently used methods for Salmonella detection are time and labour intensive. Therefore, rapid detection is a key to the prevention and identification of problems related to health and safety. This paper describes the application of a new quartz crystal microbalance (QCM) instrument with a microfluidic system for the rapid and real time detection of Salmonella Typhimurim. The QCMA-1 bare gold sensor chip which contain two sensing array was modified by covalently immobilising the monoclonal capture antibody on the active spot and a mouse IgG antibody on the control spot using a conventional amine coupling chemistry (EDC-NHS). The binding of the Salmonella cells onto the immobilised anti-Salmonella antibody alters the sensor frequency which was correlated to cells concentration in the buffer samples. Salmonella cells were detected using direct, sandwich, and sandwich assay with antibody conjugated gold-nanoparticles. The performance of the QCM immunosensor developed with gold-nanoparticles gave the highest sensitivity with a limit of detection (LOD) ~10-20 colony forming unit (CFU) ml(-1) compared to direct and sandwich assay (1.83 × 10(2) CFU ml(-1) and 1.01 × 10(2) CFU ml(-1), respectively). The sensor showed good sensitivity and selectivity for Salmonella in the presence of other bacteria in real food samples and helped in reducing the pre-enrichment step, hence, demonstrating the potential of this technology for the rapid and sensitive microbial analysis.

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Publisher Full Text

Authors+Show Affiliations

Salam F
Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL England, United Kingdom.
Uludag Y
No affiliation info available
Tothill IE
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntibodies, ImmobilizedAntibodies, MonoclonalBiosensing TechniquesChickensFood ContaminationGoldImmunoassayImmunoglobulin GLimit of DetectionMeatMetal NanoparticlesMiceMicrofluidic Analytical TechniquesQuartz Crystal Microbalance TechniquesSalmonella typhimurium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24054660

Citation

Salam, Faridah, et al. "Real-time and Sensitive Detection of Salmonella Typhimurium Using an Automated Quartz Crystal Microbalance (QCM) Instrument With Nanoparticles Amplification." Talanta, vol. 115, 2013, pp. 761-7.
Salam F, Uludag Y, Tothill IE. Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification. Talanta. 2013;115:761-7.
Salam, F., Uludag, Y., & Tothill, I. E. (2013). Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification. Talanta, 115, 761-7. https://doi.org/10.1016/j.talanta.2013.06.034
Salam F, Uludag Y, Tothill IE. Real-time and Sensitive Detection of Salmonella Typhimurium Using an Automated Quartz Crystal Microbalance (QCM) Instrument With Nanoparticles Amplification. Talanta. 2013 Oct 15;115:761-7. PubMed PMID: 24054660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification. AU - Salam,Faridah, AU - Uludag,Yildiz, AU - Tothill,Ibtisam E, Y1 - 2013/06/28/ PY - 2013/04/30/received PY - 2013/06/19/revised PY - 2013/06/21/accepted PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/4/16/medline KW - Bacteria detection KW - Food monitoring KW - Immunosensor KW - Nanoparticle KW - Quartz crystal microbalance (QCM) KW - Salmonella SP - 761 EP - 7 JF - Talanta JO - Talanta VL - 115 N2 - The accidental contamination of Salmonella in raw and processed foods is a major problem for the food industry worldwide. At present many of the currently used methods for Salmonella detection are time and labour intensive. Therefore, rapid detection is a key to the prevention and identification of problems related to health and safety. This paper describes the application of a new quartz crystal microbalance (QCM) instrument with a microfluidic system for the rapid and real time detection of Salmonella Typhimurim. The QCMA-1 bare gold sensor chip which contain two sensing array was modified by covalently immobilising the monoclonal capture antibody on the active spot and a mouse IgG antibody on the control spot using a conventional amine coupling chemistry (EDC-NHS). The binding of the Salmonella cells onto the immobilised anti-Salmonella antibody alters the sensor frequency which was correlated to cells concentration in the buffer samples. Salmonella cells were detected using direct, sandwich, and sandwich assay with antibody conjugated gold-nanoparticles. The performance of the QCM immunosensor developed with gold-nanoparticles gave the highest sensitivity with a limit of detection (LOD) ~10-20 colony forming unit (CFU) ml(-1) compared to direct and sandwich assay (1.83 × 10(2) CFU ml(-1) and 1.01 × 10(2) CFU ml(-1), respectively). The sensor showed good sensitivity and selectivity for Salmonella in the presence of other bacteria in real food samples and helped in reducing the pre-enrichment step, hence, demonstrating the potential of this technology for the rapid and sensitive microbial analysis. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24054660/Real_time_and_sensitive_detection_of_Salmonella_Typhimurium_using_an_automated_quartz_crystal_microbalance__QCM__instrument_with_nanoparticles_amplification_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(13)00534-1 DB - PRIME DP - Unbound Medicine ER -