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Antidiabetic effects of Swertia macrosperma extracts in diabetic rats.
J Ethnopharmacol. 2013 Nov 25; 150(2):536-44.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Swertia macrosperma is a traditional folk medicine used for its anti-hepatitis, antipyretic and antidotal effects as "Dida" or "Zangyinchen" in Tibet, Yunnan and Guizhou province for a long time, and it has been reported for its anti-diabetic effects in a Chinese patent. Swertia macrosperma was reported rich in xanthones, iridoids, seco-iridoids and their glycosides, several of which had been documented as potential antidiabetic agents. The objective of this study was to investigate the antidiabetic effect of Swertia macrosperma in diabetic rats.

MATERIALS AND METHODS

This study was designed firstly to evaluate the effect of Swertia macrosperma on glucose consumption in HepG2 cells. Based on the result in HepG2 cells, the antidiabetic effect of ethanol extract (EE) and n-butanol extract (BE) were investigated in diabetic rats induced by high fat fed and streptozotocin. The effects of EE and BE on fasting blood glucose, oral glucose tolerance test, serum insulin, glycosylated hemoglobin, serum lipid level, serum antioxidant parameters, glucokinase, glucose-6-phosphatase activities and glycogen content in liver tissue were measured, histology examination of pancreatic tissue was also carried out.

RESULTS

After 4 weeks treatment with EE and BE, apparently decreased fasting blood glucose concentrations were observed in these treated groups, compared with the diabetic control groups. Additionally, improvement in serum antioxidant parameters and lipid profile were evidenced clearly. Moreover, EE and BE had effects of protecting the pancreatic β-cells and stimulating insulin secretion from the remaining pancreatic β-cells, evidenced by pancreatic histology examination. Increased glucokinase activity and decreased glucose-6-phosphatase activity were observed in liver.

CONCLUSION

The results of in vivo and in vitro experiment suggested that EE and BE of Swertia macrosperma had excellent effects on controlling the hyperglycemia and hyperlipidemia in diabetic rats.

Authors+Show Affiliations

Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji School of Pharmaceutical Sciences, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24055468

Citation

Wang, Yong-Long, et al. "Antidiabetic Effects of Swertia Macrosperma Extracts in Diabetic Rats." Journal of Ethnopharmacology, vol. 150, no. 2, 2013, pp. 536-44.
Wang YL, Xiao ZQ, Liu S, et al. Antidiabetic effects of Swertia macrosperma extracts in diabetic rats. J Ethnopharmacol. 2013;150(2):536-44.
Wang, Y. L., Xiao, Z. Q., Liu, S., Wan, L. S., Yue, Y. D., Zhang, Y. T., Liu, Z. X., & Chen, J. C. (2013). Antidiabetic effects of Swertia macrosperma extracts in diabetic rats. Journal of Ethnopharmacology, 150(2), 536-44. https://doi.org/10.1016/j.jep.2013.08.053
Wang YL, et al. Antidiabetic Effects of Swertia Macrosperma Extracts in Diabetic Rats. J Ethnopharmacol. 2013 Nov 25;150(2):536-44. PubMed PMID: 24055468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antidiabetic effects of Swertia macrosperma extracts in diabetic rats. AU - Wang,Yong-Long, AU - Xiao,Zuo-Qi, AU - Liu,Shan, AU - Wan,Luo-Sheng, AU - Yue,Yao-Dong, AU - Zhang,Yu-Tang, AU - Liu,Zhao-Xia, AU - Chen,Jia-Chun, Y1 - 2013/09/17/ PY - 2013/04/24/received PY - 2013/08/03/revised PY - 2013/08/31/accepted PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/6/19/medline KW - Anti-diabetes KW - Diabetic rat KW - Extracts KW - HepG2 cell KW - Swertia macrosperma SP - 536 EP - 44 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 150 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Swertia macrosperma is a traditional folk medicine used for its anti-hepatitis, antipyretic and antidotal effects as "Dida" or "Zangyinchen" in Tibet, Yunnan and Guizhou province for a long time, and it has been reported for its anti-diabetic effects in a Chinese patent. Swertia macrosperma was reported rich in xanthones, iridoids, seco-iridoids and their glycosides, several of which had been documented as potential antidiabetic agents. The objective of this study was to investigate the antidiabetic effect of Swertia macrosperma in diabetic rats. MATERIALS AND METHODS: This study was designed firstly to evaluate the effect of Swertia macrosperma on glucose consumption in HepG2 cells. Based on the result in HepG2 cells, the antidiabetic effect of ethanol extract (EE) and n-butanol extract (BE) were investigated in diabetic rats induced by high fat fed and streptozotocin. The effects of EE and BE on fasting blood glucose, oral glucose tolerance test, serum insulin, glycosylated hemoglobin, serum lipid level, serum antioxidant parameters, glucokinase, glucose-6-phosphatase activities and glycogen content in liver tissue were measured, histology examination of pancreatic tissue was also carried out. RESULTS: After 4 weeks treatment with EE and BE, apparently decreased fasting blood glucose concentrations were observed in these treated groups, compared with the diabetic control groups. Additionally, improvement in serum antioxidant parameters and lipid profile were evidenced clearly. Moreover, EE and BE had effects of protecting the pancreatic β-cells and stimulating insulin secretion from the remaining pancreatic β-cells, evidenced by pancreatic histology examination. Increased glucokinase activity and decreased glucose-6-phosphatase activity were observed in liver. CONCLUSION: The results of in vivo and in vitro experiment suggested that EE and BE of Swertia macrosperma had excellent effects on controlling the hyperglycemia and hyperlipidemia in diabetic rats. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24055468/Antidiabetic_effects_of_Swertia_macrosperma_extracts_in_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00624-7 DB - PRIME DP - Unbound Medicine ER -