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Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood.

Abstract

Ultra-high performance liquid chromatography (UHPLC) was applied for the first time in the analysis of wood extracts. The potential of this technique coupled to ion trap mass spectrometry in the rapid and effective detection and identification of bioactive components in complex vegetal samples was demonstrated. Several dozens of compounds were detected in less than 30min of analysis time, corresponding to more than 3-fold reduction in time, when compared to conventional HPLC analysis of similar extracts. The phenolic chemical composition of Eucalyptus grandis, Eucalyptus urograndis (E. grandis×E. urophylla) and Eucalyptus maidenii wood extracts was assessed for the first time, with the identification of 51 phenolic compounds in the three wood extracts. Twenty of these compounds are reported for the first time as Eucalyptus genus components. Ellagic acid and ellagic acid-pentoside are the major components in all extracts, followed by gallic and quinic acids in E. grandis and E. urograndis and ellagic acid-pentoside isomer, isorhamnetin-hexoside and gallic acid in E. maidenii. The antioxidant scavenging activity of the extracts was evaluated, with E. grandis wood extract showing the lowest IC50 value. Moreover, the antioxidant activity of these extracts was higher than that of the commercial antioxidant BHT and of those of the corresponding bark extracts. These results, together with the phenolic content values, open good perspectives for the exploitation of these renewable resources as a source of valuable phenolic compounds.

Authors+Show Affiliations

CICECO and Chemistry Department, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. Electronic address: santos.sonia@ua.pt.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24055752

Citation

Santos, Sónia A O., et al. "Ultra-high Performance Liquid Chromatography Coupled to Mass Spectrometry Applied to the Identification of Valuable Phenolic Compounds From Eucalyptus Wood." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 938, 2013, pp. 65-74.
Santos SA, Vilela C, Freire CS, et al. Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;938:65-74.
Santos, S. A., Vilela, C., Freire, C. S., Neto, C. P., & Silvestre, A. J. (2013). Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 938, 65-74. https://doi.org/10.1016/j.jchromb.2013.08.034
Santos SA, et al. Ultra-high Performance Liquid Chromatography Coupled to Mass Spectrometry Applied to the Identification of Valuable Phenolic Compounds From Eucalyptus Wood. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 1;938:65-74. PubMed PMID: 24055752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood. AU - Santos,Sónia A O, AU - Vilela,Carla, AU - Freire,Carmen S R, AU - Neto,Carlos Pascoal, AU - Silvestre,Armando J D, Y1 - 2013/09/01/ PY - 2013/05/27/received PY - 2013/08/23/revised PY - 2013/08/27/accepted PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/3/19/medline KW - Eucalyptus grandis KW - Eucalyptus maidenii KW - Eucalyptus urograndis KW - Phenolic compounds KW - UHPLC KW - Wood extracts SP - 65 EP - 74 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 938 N2 - Ultra-high performance liquid chromatography (UHPLC) was applied for the first time in the analysis of wood extracts. The potential of this technique coupled to ion trap mass spectrometry in the rapid and effective detection and identification of bioactive components in complex vegetal samples was demonstrated. Several dozens of compounds were detected in less than 30min of analysis time, corresponding to more than 3-fold reduction in time, when compared to conventional HPLC analysis of similar extracts. The phenolic chemical composition of Eucalyptus grandis, Eucalyptus urograndis (E. grandis×E. urophylla) and Eucalyptus maidenii wood extracts was assessed for the first time, with the identification of 51 phenolic compounds in the three wood extracts. Twenty of these compounds are reported for the first time as Eucalyptus genus components. Ellagic acid and ellagic acid-pentoside are the major components in all extracts, followed by gallic and quinic acids in E. grandis and E. urograndis and ellagic acid-pentoside isomer, isorhamnetin-hexoside and gallic acid in E. maidenii. The antioxidant scavenging activity of the extracts was evaluated, with E. grandis wood extract showing the lowest IC50 value. Moreover, the antioxidant activity of these extracts was higher than that of the commercial antioxidant BHT and of those of the corresponding bark extracts. These results, together with the phenolic content values, open good perspectives for the exploitation of these renewable resources as a source of valuable phenolic compounds. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24055752/Ultra_high_performance_liquid_chromatography_coupled_to_mass_spectrometry_applied_to_the_identification_of_valuable_phenolic_compounds_from_Eucalyptus_wood_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00462-5 DB - PRIME DP - Unbound Medicine ER -