Tags

Type your tag names separated by a space and hit enter

Components of the cultivated red seaweed Chondrus crispus enhance the immune response of Caenorhabditis elegans to Pseudomonas aeruginosa through the pmk-1, daf-2/daf-16, and skn-1 pathways.
Appl Environ Microbiol. 2013 Dec; 79(23):7343-50.AE

Abstract

Marine macroalgae are rich in bioactive compounds that can, when consumed, impart beneficial effects on animal and human health. The red seaweed Chondrus crispus has been reported to have a wide range of health-promoting activities, such as antitumor and antiviral activities. Using a Caenorhabditis elegans infection model, we show that C. crispus water extract (CCWE) enhances host immunity and suppresses the expression of quorum sensing (QS) and the virulence factors of Pseudomonas aeruginosa (strain PA14). Supplementation of nematode growth medium with CCWE induced the expression of C. elegans innate immune genes, such as irg-1, irg-2, F49F1.6, hsf-1, K05D8.5, F56D6.2, C29F3.7, F28D1.3, F38A1.5 ZK6.7, lys-1, spp-1, and abf-1, by more than 2-fold, while T20G5.7 was not affected. Additionally, CCWE suppressed the expression of PA14 QS genes and virulence factors, although it did not affect the growth of the bacteria. These effects correlated with a 28% reduction in the PA14-inflicted killing of C. elegans. Kappa-carrageenan (K-CGN), a major component of CCWE, was shown to play an important role in the enhancement of host immunity. Using C. elegans mutants, we identified that pmk-1, daf-2/daf-16, and skn-1 are essential in the K-CGN-induced host immune response. In view of the conservation of innate immune pathways between C. elegans and humans, the results of this study suggest that water-soluble components of C. crispus may also play a health-promoting role in higher animals and humans.

Links

PMC Free PDF
PMC Free Full Text
Publisher Full Text

Authors+Show Affiliations

Liu J
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, Canada.
Hafting J
No affiliation info available
Critchley AT
No affiliation info available
Banskota AH
No affiliation info available
Prithiviraj B
No affiliation info available

MeSH

AnimalsAnti-Bacterial AgentsCaenorhabditis elegansCaenorhabditis elegans ProteinsChondrusDNA-Binding ProteinsForkhead Transcription FactorsImmunity, InnateImmunologic FactorsMitogen-Activated Protein KinasesPlant ExtractsPseudomonas aeruginosaQuorum SensingReceptor, InsulinTranscription FactorsVirulence Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24056462

Citation

Liu, Jinghua, et al. "Components of the Cultivated Red Seaweed Chondrus Crispus Enhance the Immune Response of Caenorhabditis Elegans to Pseudomonas Aeruginosa Through the Pmk-1, Daf-2/daf-16, and Skn-1 Pathways." Applied and Environmental Microbiology, vol. 79, no. 23, 2013, pp. 7343-50.
Liu J, Hafting J, Critchley AT, et al. Components of the cultivated red seaweed Chondrus crispus enhance the immune response of Caenorhabditis elegans to Pseudomonas aeruginosa through the pmk-1, daf-2/daf-16, and skn-1 pathways. Appl Environ Microbiol. 2013;79(23):7343-50.
Liu, J., Hafting, J., Critchley, A. T., Banskota, A. H., & Prithiviraj, B. (2013). Components of the cultivated red seaweed Chondrus crispus enhance the immune response of Caenorhabditis elegans to Pseudomonas aeruginosa through the pmk-1, daf-2/daf-16, and skn-1 pathways. Applied and Environmental Microbiology, 79(23), 7343-50. https://doi.org/10.1128/AEM.01927-13
Liu J, et al. Components of the Cultivated Red Seaweed Chondrus Crispus Enhance the Immune Response of Caenorhabditis Elegans to Pseudomonas Aeruginosa Through the Pmk-1, Daf-2/daf-16, and Skn-1 Pathways. Appl Environ Microbiol. 2013;79(23):7343-50. PubMed PMID: 24056462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Components of the cultivated red seaweed Chondrus crispus enhance the immune response of Caenorhabditis elegans to Pseudomonas aeruginosa through the pmk-1, daf-2/daf-16, and skn-1 pathways. AU - Liu,Jinghua, AU - Hafting,Jeff, AU - Critchley,Alan T, AU - Banskota,Arjun H, AU - Prithiviraj,Balakrishnan, Y1 - 2013/09/20/ PY - 2013/9/24/entrez PY - 2013/9/24/pubmed PY - 2014/6/5/medline SP - 7343 EP - 50 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 79 IS - 23 N2 - Marine macroalgae are rich in bioactive compounds that can, when consumed, impart beneficial effects on animal and human health. The red seaweed Chondrus crispus has been reported to have a wide range of health-promoting activities, such as antitumor and antiviral activities. Using a Caenorhabditis elegans infection model, we show that C. crispus water extract (CCWE) enhances host immunity and suppresses the expression of quorum sensing (QS) and the virulence factors of Pseudomonas aeruginosa (strain PA14). Supplementation of nematode growth medium with CCWE induced the expression of C. elegans innate immune genes, such as irg-1, irg-2, F49F1.6, hsf-1, K05D8.5, F56D6.2, C29F3.7, F28D1.3, F38A1.5 ZK6.7, lys-1, spp-1, and abf-1, by more than 2-fold, while T20G5.7 was not affected. Additionally, CCWE suppressed the expression of PA14 QS genes and virulence factors, although it did not affect the growth of the bacteria. These effects correlated with a 28% reduction in the PA14-inflicted killing of C. elegans. Kappa-carrageenan (K-CGN), a major component of CCWE, was shown to play an important role in the enhancement of host immunity. Using C. elegans mutants, we identified that pmk-1, daf-2/daf-16, and skn-1 are essential in the K-CGN-induced host immune response. In view of the conservation of innate immune pathways between C. elegans and humans, the results of this study suggest that water-soluble components of C. crispus may also play a health-promoting role in higher animals and humans. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/24056462/Components_of_the_cultivated_red_seaweed_Chondrus_crispus_enhance_the_immune_response_of_Caenorhabditis_elegans_to_Pseudomonas_aeruginosa_through_the_pmk_1_daf_2/daf_16_and_skn_1_pathways_ L2 - https://journals.asm.org/doi/10.1128/AEM.01927-13?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -