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Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts.
J Ethnopharmacol. 2013 Nov 25; 150(2):609-13.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant.

MATERIALS AND METHODS

The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses.

RESULTS

Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: myricetin-3-O-arabinopyranoside (2), myricetrin-3-O-rhamnoside (3), kaempferol-3-O-rhamnoside (4) and quercetin-3-O-arabinofuranoside (5). These compounds are reported from Searsia chirindensis for the first time. All the compounds showed good antibacterial activity against all bacterial strains tested. Their minimum inhibitory concentrations ranged from 30 to 250 µg/mL.

CONCLUSIONS

Antibacterial activity demonstrated by the extracts and isolated compounds provides credence to the ethnomedicinal use of Searsia chirindensis against diarrhoea.

Authors+Show Affiliations

Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24060408

Citation

Madikizela, Balungile, et al. "Isolation and Characterization of Antimicrobial Constituents of Searsia Chirindensis L. (Anacardiaceae) Leaf Extracts." Journal of Ethnopharmacology, vol. 150, no. 2, 2013, pp. 609-13.
Madikizela B, Aderogba MA, Van Staden J. Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts. J Ethnopharmacol. 2013;150(2):609-13.
Madikizela, B., Aderogba, M. A., & Van Staden, J. (2013). Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts. Journal of Ethnopharmacology, 150(2), 609-13. https://doi.org/10.1016/j.jep.2013.09.016
Madikizela B, Aderogba MA, Van Staden J. Isolation and Characterization of Antimicrobial Constituents of Searsia Chirindensis L. (Anacardiaceae) Leaf Extracts. J Ethnopharmacol. 2013 Nov 25;150(2):609-13. PubMed PMID: 24060408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts. AU - Madikizela,Balungile, AU - Aderogba,Mutalib A, AU - Van Staden,Johannes, Y1 - 2013/09/21/ PY - 2013/07/19/received PY - 2013/08/30/revised PY - 2013/09/06/accepted PY - 2013/9/25/entrez PY - 2013/9/26/pubmed PY - 2014/6/19/medline KW - AIDS KW - ATCC KW - American type culture collection KW - Anacardiaceae KW - Antimicrobial KW - CFU KW - DCM KW - Diarrhoea KW - EtOAc KW - Flavonol glycosides KW - HIV KW - INT KW - MH KW - MHz KW - MIC KW - MeOH KW - Mueller-hinton KW - NMR KW - No.1 KW - Searsia chirindensis KW - TLC KW - UKZN KW - University of KwaZulu-Natal KW - WHO KW - World Health Organization KW - acquired immunodeficiency syndrome KW - colony forming unit KW - dichloromethane KW - ethyl acetate KW - human immunodeficiency virus KW - iodonitrotetrazolium chloride KW - megahertz KW - methanol KW - minimum inhibition concentration KW - nuclear magnetic resonance KW - number 1 KW - part per million KW - ppm KW - thin layer chromatography SP - 609 EP - 13 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 150 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant. MATERIALS AND METHODS: The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses. RESULTS: Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: myricetin-3-O-arabinopyranoside (2), myricetrin-3-O-rhamnoside (3), kaempferol-3-O-rhamnoside (4) and quercetin-3-O-arabinofuranoside (5). These compounds are reported from Searsia chirindensis for the first time. All the compounds showed good antibacterial activity against all bacterial strains tested. Their minimum inhibitory concentrations ranged from 30 to 250 µg/mL. CONCLUSIONS: Antibacterial activity demonstrated by the extracts and isolated compounds provides credence to the ethnomedicinal use of Searsia chirindensis against diarrhoea. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24060408/Isolation_and_characterization_of_antimicrobial_constituents_of_Searsia_chirindensis_L___Anacardiaceae__leaf_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00649-1 DB - PRIME DP - Unbound Medicine ER -