Abstract
ETHNOPHARMACOLOGICAL RELEVANCE
Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant.
MATERIALS AND METHODS
The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses.
RESULTS
Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: myricetin-3-O-arabinopyranoside (2), myricetrin-3-O-rhamnoside (3), kaempferol-3-O-rhamnoside (4) and quercetin-3-O-arabinofuranoside (5). These compounds are reported from Searsia chirindensis for the first time. All the compounds showed good antibacterial activity against all bacterial strains tested. Their minimum inhibitory concentrations ranged from 30 to 250 µg/mL.
CONCLUSIONS
Antibacterial activity demonstrated by the extracts and isolated compounds provides credence to the ethnomedicinal use of Searsia chirindensis against diarrhoea.
TY - JOUR
T1 - Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts.
AU - Madikizela,Balungile,
AU - Aderogba,Mutalib A,
AU - Van Staden,Johannes,
Y1 - 2013/09/21/
PY - 2013/07/19/received
PY - 2013/08/30/revised
PY - 2013/09/06/accepted
PY - 2013/9/25/entrez
PY - 2013/9/26/pubmed
PY - 2014/6/19/medline
KW - AIDS
KW - ATCC
KW - American type culture collection
KW - Anacardiaceae
KW - Antimicrobial
KW - CFU
KW - DCM
KW - Diarrhoea
KW - EtOAc
KW - Flavonol glycosides
KW - HIV
KW - INT
KW - MH
KW - MHz
KW - MIC
KW - MeOH
KW - Mueller-hinton
KW - NMR
KW - No.1
KW - Searsia chirindensis
KW - TLC
KW - UKZN
KW - University of KwaZulu-Natal
KW - WHO
KW - World Health Organization
KW - acquired immunodeficiency syndrome
KW - colony forming unit
KW - dichloromethane
KW - ethyl acetate
KW - human immunodeficiency virus
KW - iodonitrotetrazolium chloride
KW - megahertz
KW - methanol
KW - minimum inhibition concentration
KW - nuclear magnetic resonance
KW - number 1
KW - part per million
KW - ppm
KW - thin layer chromatography
SP - 609
EP - 13
JF - Journal of ethnopharmacology
JO - J Ethnopharmacol
VL - 150
IS - 2
N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant. MATERIALS AND METHODS: The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses. RESULTS: Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: myricetin-3-O-arabinopyranoside (2), myricetrin-3-O-rhamnoside (3), kaempferol-3-O-rhamnoside (4) and quercetin-3-O-arabinofuranoside (5). These compounds are reported from Searsia chirindensis for the first time. All the compounds showed good antibacterial activity against all bacterial strains tested. Their minimum inhibitory concentrations ranged from 30 to 250 µg/mL. CONCLUSIONS: Antibacterial activity demonstrated by the extracts and isolated compounds provides credence to the ethnomedicinal use of Searsia chirindensis against diarrhoea.
SN - 1872-7573
UR - https://www.unboundmedicine.com/medline/citation/24060408/Isolation_and_characterization_of_antimicrobial_constituents_of_Searsia_chirindensis_L___Anacardiaceae__leaf_extracts_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00649-1
DB - PRIME
DP - Unbound Medicine
ER -
