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Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99.
Theor Appl Genet. 2013 Dec; 126(12):3081-9.TA

Abstract

KEY MESSAGE

The effectiveness of wheat cultivar Liangxing 99 against powdery mildew was shown to be controlled by a single dominant gene located on a new locus of chromosome 2BL in the bin 2BL2-0.35-0.50. Liangxing 99, one of the most widely grown commercial cultivars in the winter wheat (Triticum aestivum) producing regions in northern China, was shown to provide a broad spectrum of resistance to Blumeria graminis f. sp. tritici (Bgt) isolates originating from that region. Using an F2 population and F2:3 lines derived from a cross of Liangxing 99 × Zhongzuo 9504, genetic analysis demonstrated that a single dominant gene, designated MlLX99, was responsible for the resistance of Liangxing 99 to Bgt isolate E09. The results of molecular analysis indicated that this gene is located on chromosome 2BL and flanked by the SSR marker Xgwm120 and EST-STS marker BE604758 at genetic distances of 2.9 and 5.5 cM, respectively. Since the flanking markers of MlLX99 were previously mapped to the bin 2BL2-0.36-0.50, MlLX99 must be located in this chromosomal region. MlLX99 showed a different resistance reaction pattern to 60 Bgt isolates from Pm6, Pm33, and PmJM22, which were all previously mapped on chromosome 2BL, but differed in their positions from MlLX99. Due to its unique position on chromosome 2BL, MlLX99 appears to be a new locus for resistance to powdery mildew. Liangxing 99 has shown superior yield performance and wide adaptation to different agricultural conditions, which has resulted in its extensive use as a wheat cultivar in China. The identification of resistance gene MlLX99 facilitates the use of this cultivar in the protection of wheat from damage caused by powdery mildew.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhao Z
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Sun H
No affiliation info available
Song W
No affiliation info available
Lu M
No affiliation info available
Huang J
No affiliation info available
Wu L
No affiliation info available
Wang X
No affiliation info available
Li H
No affiliation info available

MeSH

AscomycotaChromosome MappingChromosomes, PlantDNA, PlantDisease ResistanceGenes, PlantGenetic LinkageGenetic MarkersImmunity, InnatePlant DiseasesTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24061485

Citation

Zhao, Zihui, et al. "Genetic Analysis and Detection of the Gene MlLX99 On Chromosome 2BL Conferring Resistance to Powdery Mildew in the Wheat Cultivar Liangxing 99." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 126, no. 12, 2013, pp. 3081-9.
Zhao Z, Sun H, Song W, et al. Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99. Theor Appl Genet. 2013;126(12):3081-9.
Zhao, Z., Sun, H., Song, W., Lu, M., Huang, J., Wu, L., Wang, X., & Li, H. (2013). Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 126(12), 3081-9. https://doi.org/10.1007/s00122-013-2194-6
Zhao Z, et al. Genetic Analysis and Detection of the Gene MlLX99 On Chromosome 2BL Conferring Resistance to Powdery Mildew in the Wheat Cultivar Liangxing 99. Theor Appl Genet. 2013;126(12):3081-9. PubMed PMID: 24061485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99. AU - Zhao,Zihui, AU - Sun,Huigai, AU - Song,Wei, AU - Lu,Ming, AU - Huang,Jiang, AU - Wu,Longfei, AU - Wang,Xiaoming, AU - Li,Hongjie, Y1 - 2013/09/24/ PY - 2013/04/14/received PY - 2013/09/06/accepted PY - 2013/9/25/entrez PY - 2013/9/26/pubmed PY - 2014/7/18/medline SP - 3081 EP - 9 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 126 IS - 12 N2 - KEY MESSAGE: The effectiveness of wheat cultivar Liangxing 99 against powdery mildew was shown to be controlled by a single dominant gene located on a new locus of chromosome 2BL in the bin 2BL2-0.35-0.50. Liangxing 99, one of the most widely grown commercial cultivars in the winter wheat (Triticum aestivum) producing regions in northern China, was shown to provide a broad spectrum of resistance to Blumeria graminis f. sp. tritici (Bgt) isolates originating from that region. Using an F2 population and F2:3 lines derived from a cross of Liangxing 99 × Zhongzuo 9504, genetic analysis demonstrated that a single dominant gene, designated MlLX99, was responsible for the resistance of Liangxing 99 to Bgt isolate E09. The results of molecular analysis indicated that this gene is located on chromosome 2BL and flanked by the SSR marker Xgwm120 and EST-STS marker BE604758 at genetic distances of 2.9 and 5.5 cM, respectively. Since the flanking markers of MlLX99 were previously mapped to the bin 2BL2-0.36-0.50, MlLX99 must be located in this chromosomal region. MlLX99 showed a different resistance reaction pattern to 60 Bgt isolates from Pm6, Pm33, and PmJM22, which were all previously mapped on chromosome 2BL, but differed in their positions from MlLX99. Due to its unique position on chromosome 2BL, MlLX99 appears to be a new locus for resistance to powdery mildew. Liangxing 99 has shown superior yield performance and wide adaptation to different agricultural conditions, which has resulted in its extensive use as a wheat cultivar in China. The identification of resistance gene MlLX99 facilitates the use of this cultivar in the protection of wheat from damage caused by powdery mildew. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/24061485/Genetic_analysis_and_detection_of_the_gene_MlLX99_on_chromosome_2BL_conferring_resistance_to_powdery_mildew_in_the_wheat_cultivar_Liangxing_99_ L2 - https://dx.doi.org/10.1007/s00122-013-2194-6 DB - PRIME DP - Unbound Medicine ER -