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Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST.
ASN Neuro 2013; 5(4):283-9AN

Abstract

LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly in rat brain. We currently analyzed whether focal ischemia also alters the association of lncRNAs with the CMPs Sin3A and coREST (corepressors of the RE-1 silencing transcription factor). RIP (RNA immunoprecipitation) combined with lncRNA microarray analysis showed that 177 of the 2497 lncRNAs expressed in rat cerebral cortex showed significantly increased binding to either Sin3A or coREST following ischemia compared with sham. Of these, 26 lncRNAs enriched with Sin3A and 11 lncRNAs enriched with coREST were also up-regulated in their expressions after ischemia. A majority of the lncRNAs enriched with these CMPs were intergenic in origin. Evaluation of the expression profiles of corresponding protein-coding genes showed that their expression levels correlate with those of the lncRNAs with which they shared a common locus. This is the first study to show that stroke-induced lncRNAs might associate with CMPs to modulate the post-ischemic epigenetic landscape.

Authors+Show Affiliations

*Department of Neurological Surgery, University of Wisconsin, Madison, WI, U.S.A.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24063527

Citation

Dharap, Ashutosh, et al. "Increased Binding of Stroke-induced Long Non-coding RNAs to the Transcriptional Corepressors Sin3A and CoREST." ASN Neuro, vol. 5, no. 4, 2013, pp. 283-9.
Dharap A, Pokrzywa C, Vemuganti R. Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST. ASN Neuro. 2013;5(4):283-9.
Dharap, A., Pokrzywa, C., & Vemuganti, R. (2013). Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST. ASN Neuro, 5(4), pp. 283-9. doi:10.1042/AN20130029.
Dharap A, Pokrzywa C, Vemuganti R. Increased Binding of Stroke-induced Long Non-coding RNAs to the Transcriptional Corepressors Sin3A and CoREST. ASN Neuro. 2013 Oct 23;5(4):283-9. PubMed PMID: 24063527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased binding of stroke-induced long non-coding RNAs to the transcriptional corepressors Sin3A and coREST. AU - Dharap,Ashutosh, AU - Pokrzywa,Courtney, AU - Vemuganti,Raghu, Y1 - 2013/10/23/ PY - 2013/9/26/entrez PY - 2013/9/26/pubmed PY - 2014/6/16/medline SP - 283 EP - 9 JF - ASN neuro JO - ASN Neuro VL - 5 IS - 4 N2 - LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly in rat brain. We currently analyzed whether focal ischemia also alters the association of lncRNAs with the CMPs Sin3A and coREST (corepressors of the RE-1 silencing transcription factor). RIP (RNA immunoprecipitation) combined with lncRNA microarray analysis showed that 177 of the 2497 lncRNAs expressed in rat cerebral cortex showed significantly increased binding to either Sin3A or coREST following ischemia compared with sham. Of these, 26 lncRNAs enriched with Sin3A and 11 lncRNAs enriched with coREST were also up-regulated in their expressions after ischemia. A majority of the lncRNAs enriched with these CMPs were intergenic in origin. Evaluation of the expression profiles of corresponding protein-coding genes showed that their expression levels correlate with those of the lncRNAs with which they shared a common locus. This is the first study to show that stroke-induced lncRNAs might associate with CMPs to modulate the post-ischemic epigenetic landscape. SN - 1759-0914 UR - https://www.unboundmedicine.com/medline/citation/24063527/Increased_binding_of_stroke_induced_long_non_coding_RNAs_to_the_transcriptional_corepressors_Sin3A_and_coREST_ L2 - http://journals.sagepub.com/doi/full/10.1042/AN20130029?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -