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A study of changing trends of prevalence and genotypic distribution of hepatitis C virus among high risk groups in North India.
Indian J Med Microbiol. 2013 Oct-Dec; 31(4):354-9.IJ

Abstract

PURPOSE

Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. There is a great variability in HCV's geographical presence, transmission routes, genotypic distribution etc., in studied populations. We undertook this study in a North Indian hospital on patients of chronic liver disease to observe any emerging trend in risk groups, transmission patterns, genotypic distribution of the virus in this geographical region and its correlation with viral load.

MATERIALS AND METHODS

There were 54 anti-HCV positive patients including 31 HCV Ribonucleic acid (RNA) positive patients were included in the study. HCV genotyping was carried out by restriction fragment length polymorphism (RFLP) followed by direct sequencing of the core region. Viral load estimation was carried out by Taqman real time polymerase chain reaction system.

RESULTS

In 45/54 (83.3%) anti-HCV positive patients, iatrogenic procedures were responsible for transmission with blood transfusion alone responsible in 36/54 (67%). Genotype 3 was observed to be the commonest type found in all risk groups followed by type 1 and 2. Subtype 3b (35.5%) was found more prevalent than subtype 3a. A higher frequency of subtype 1b (19.4%) was also seen. Genotype 1 was associated with a significantly higher viral load compared to genotypes 3 and 2. No significant difference was observed in the biochemical profile among the three genotypes except for the levels of the enzyme, aspartate aminotransferase (AST).

CONCLUSIONS

Iatrogenic procedures, especially contaminated blood transfusion etc., still contributes significantly to the pool of HCV infection. Genotype 3 is the predominant genotype in North India, though the subtype distribution within genotype 3 may be changing. The association of severe liver disease is significantly more with genotype 1 as evidenced by higher viral load and deranged AST levels.

Authors+Show Affiliations

Department of Microbiology, Maulana Azad Medical College, New Delhi, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24064641

Citation

Chakravarti, A, et al. "A Study of Changing Trends of Prevalence and Genotypic Distribution of Hepatitis C Virus Among High Risk Groups in North India." Indian Journal of Medical Microbiology, vol. 31, no. 4, 2013, pp. 354-9.
Chakravarti A, Ashraf A, Malik S. A study of changing trends of prevalence and genotypic distribution of hepatitis C virus among high risk groups in North India. Indian J Med Microbiol. 2013;31(4):354-9.
Chakravarti, A., Ashraf, A., & Malik, S. (2013). A study of changing trends of prevalence and genotypic distribution of hepatitis C virus among high risk groups in North India. Indian Journal of Medical Microbiology, 31(4), 354-9. https://doi.org/10.4103/0255-0857.118877
Chakravarti A, Ashraf A, Malik S. A Study of Changing Trends of Prevalence and Genotypic Distribution of Hepatitis C Virus Among High Risk Groups in North India. Indian J Med Microbiol. 2013 Oct-Dec;31(4):354-9. PubMed PMID: 24064641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A study of changing trends of prevalence and genotypic distribution of hepatitis C virus among high risk groups in North India. AU - Chakravarti,A, AU - Ashraf,A, AU - Malik,S, PY - 2013/9/26/entrez PY - 2013/9/26/pubmed PY - 2015/5/21/medline SP - 354 EP - 9 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 31 IS - 4 N2 - PURPOSE: Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. There is a great variability in HCV's geographical presence, transmission routes, genotypic distribution etc., in studied populations. We undertook this study in a North Indian hospital on patients of chronic liver disease to observe any emerging trend in risk groups, transmission patterns, genotypic distribution of the virus in this geographical region and its correlation with viral load. MATERIALS AND METHODS: There were 54 anti-HCV positive patients including 31 HCV Ribonucleic acid (RNA) positive patients were included in the study. HCV genotyping was carried out by restriction fragment length polymorphism (RFLP) followed by direct sequencing of the core region. Viral load estimation was carried out by Taqman real time polymerase chain reaction system. RESULTS: In 45/54 (83.3%) anti-HCV positive patients, iatrogenic procedures were responsible for transmission with blood transfusion alone responsible in 36/54 (67%). Genotype 3 was observed to be the commonest type found in all risk groups followed by type 1 and 2. Subtype 3b (35.5%) was found more prevalent than subtype 3a. A higher frequency of subtype 1b (19.4%) was also seen. Genotype 1 was associated with a significantly higher viral load compared to genotypes 3 and 2. No significant difference was observed in the biochemical profile among the three genotypes except for the levels of the enzyme, aspartate aminotransferase (AST). CONCLUSIONS: Iatrogenic procedures, especially contaminated blood transfusion etc., still contributes significantly to the pool of HCV infection. Genotype 3 is the predominant genotype in North India, though the subtype distribution within genotype 3 may be changing. The association of severe liver disease is significantly more with genotype 1 as evidenced by higher viral load and deranged AST levels. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/24064641/A_study_of_changing_trends_of_prevalence_and_genotypic_distribution_of_hepatitis_C_virus_among_high_risk_groups_in_North_India_ L2 - http://www.ijmm.org/article.asp?issn=0255-0857;year=2013;volume=31;issue=4;spage=354;epage=359;aulast=Chakravarti DB - PRIME DP - Unbound Medicine ER -