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A 3' splice site-binding sequence in the catalytic core of a group I intron.
Nature. 1990 Mar 01; 344(6261):80-2.Nat

Abstract

Ribozymes use specific RNA-RNA interactions for substrate binding and active-site formation. Self-splicing group I introns have approximately 70 nucleotides constituting the core, a region containing sequences and structures indispensable for catalytic function. The catalytic core must interact with the substrates used for the two steps of the self-splicing reaction, that is, guanosine, the 5'-splice-site helix (P1) and the 3' splice site. Mutational evidence suggests that core sequences near segment J6/7 that joins the base-paired stems P6 and P7, and the bulged base of P7(5'), participate in binding guanosine substrate, but nothing is known about the interactions between the core, the 5'-splice-site helix and the 3' splice site. On the basis of comparative sequence data, it has been suggested that two specific bases in the catalytic core of group I introns might form a binding sequence for the 3' splice site. Here we present genetic evidence that such a binding site exists in the core of the Tetrahymena large subunit ribosomal RNA intron. We demonstrate that this pairing, termed P9.0, is functionally important in the exon ligation step of self-splicing, but is not itself responsible for 3'-splice-site selection.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2406615

Citation

Burke, J M., et al. "A 3' Splice Site-binding Sequence in the Catalytic Core of a Group I Intron." Nature, vol. 344, no. 6261, 1990, pp. 80-2.
Burke JM, Esherick JS, Burfeind WR, et al. A 3' splice site-binding sequence in the catalytic core of a group I intron. Nature. 1990;344(6261):80-2.
Burke, J. M., Esherick, J. S., Burfeind, W. R., & King, J. L. (1990). A 3' splice site-binding sequence in the catalytic core of a group I intron. Nature, 344(6261), 80-2.
Burke JM, et al. A 3' Splice Site-binding Sequence in the Catalytic Core of a Group I Intron. Nature. 1990 Mar 1;344(6261):80-2. PubMed PMID: 2406615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A 3' splice site-binding sequence in the catalytic core of a group I intron. AU - Burke,J M, AU - Esherick,J S, AU - Burfeind,W R, AU - King,J L, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 80 EP - 2 JF - Nature JO - Nature VL - 344 IS - 6261 N2 - Ribozymes use specific RNA-RNA interactions for substrate binding and active-site formation. Self-splicing group I introns have approximately 70 nucleotides constituting the core, a region containing sequences and structures indispensable for catalytic function. The catalytic core must interact with the substrates used for the two steps of the self-splicing reaction, that is, guanosine, the 5'-splice-site helix (P1) and the 3' splice site. Mutational evidence suggests that core sequences near segment J6/7 that joins the base-paired stems P6 and P7, and the bulged base of P7(5'), participate in binding guanosine substrate, but nothing is known about the interactions between the core, the 5'-splice-site helix and the 3' splice site. On the basis of comparative sequence data, it has been suggested that two specific bases in the catalytic core of group I introns might form a binding sequence for the 3' splice site. Here we present genetic evidence that such a binding site exists in the core of the Tetrahymena large subunit ribosomal RNA intron. We demonstrate that this pairing, termed P9.0, is functionally important in the exon ligation step of self-splicing, but is not itself responsible for 3'-splice-site selection. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/2406615/A_3'_splice_site_binding_sequence_in_the_catalytic_core_of_a_group_I_intron_ L2 - https://doi.org/10.1038/344080a0 DB - PRIME DP - Unbound Medicine ER -