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A novel ruthenium(II)-polypyridyl complex inhibits cell proliferation and induces cell apoptosis by impairing DNA damage repair.
J Chemother. 2014 Aug; 26(4):235-42.JC

Abstract

Ruthenium complexes are widely recognized as one of the most promising DNA damaging chemotherapeutic drugs. The main goal of this study was to explore the anticancer activity and underlying mechanisms of [Ru(phen)(2)(p-BrPIP)](ClO(4))(2), a novel chemically synthesized ruthenium (Ru) complex. To this end, we employed MTT assays to determine the anticancer activity of the complex, and performed single-cell gel electrophoresis (SCGE) and Western blotting to evaluate DNA damage. Our results showed that the Ru(II)-poly complex caused severe DNA damage, possibly by downregulating key factors involved in DNA repair pathways, such as proliferating cell nuclear antigen (PCNA) and ring finger protein 8 (RNF8). In addition, this complex induced cell apoptosis by upregulating both p21 and p53. Taken together, our findings demonstrate that the Ru(II)-poly complex exhibits antitumour activity by inducing cell apoptosis, which results from the accumulation of large amounts of unrepaired DNA damage.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24070188

Citation

Yang, Qingyuan, et al. "A Novel ruthenium(II)-polypyridyl Complex Inhibits Cell Proliferation and Induces Cell Apoptosis By Impairing DNA Damage Repair." Journal of Chemotherapy (Florence, Italy), vol. 26, no. 4, 2014, pp. 235-42.
Yang Q, Zhang Z, Mei W, et al. A novel ruthenium(II)-polypyridyl complex inhibits cell proliferation and induces cell apoptosis by impairing DNA damage repair. J Chemother. 2014;26(4):235-42.
Yang, Q., Zhang, Z., Mei, W., & Sun, F. (2014). A novel ruthenium(II)-polypyridyl complex inhibits cell proliferation and induces cell apoptosis by impairing DNA damage repair. Journal of Chemotherapy (Florence, Italy), 26(4), 235-42. https://doi.org/10.1179/1973947813Y.0000000138
Yang Q, et al. A Novel ruthenium(II)-polypyridyl Complex Inhibits Cell Proliferation and Induces Cell Apoptosis By Impairing DNA Damage Repair. J Chemother. 2014;26(4):235-42. PubMed PMID: 24070188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel ruthenium(II)-polypyridyl complex inhibits cell proliferation and induces cell apoptosis by impairing DNA damage repair. AU - Yang,Qingyuan, AU - Zhang,Zhao, AU - Mei,Wenjie, AU - Sun,Fenyong, Y1 - 2013/12/06/ PY - 2013/9/28/entrez PY - 2013/9/28/pubmed PY - 2015/3/31/medline KW - Antitumour, KW - DNA repair, KW - PCNA, KW - Pro-apoptosis, KW - Proliferation, KW - RNF8, KW - Ruthenium, KW - Therapy SP - 235 EP - 42 JF - Journal of chemotherapy (Florence, Italy) JO - J Chemother VL - 26 IS - 4 N2 - Ruthenium complexes are widely recognized as one of the most promising DNA damaging chemotherapeutic drugs. The main goal of this study was to explore the anticancer activity and underlying mechanisms of [Ru(phen)(2)(p-BrPIP)](ClO(4))(2), a novel chemically synthesized ruthenium (Ru) complex. To this end, we employed MTT assays to determine the anticancer activity of the complex, and performed single-cell gel electrophoresis (SCGE) and Western blotting to evaluate DNA damage. Our results showed that the Ru(II)-poly complex caused severe DNA damage, possibly by downregulating key factors involved in DNA repair pathways, such as proliferating cell nuclear antigen (PCNA) and ring finger protein 8 (RNF8). In addition, this complex induced cell apoptosis by upregulating both p21 and p53. Taken together, our findings demonstrate that the Ru(II)-poly complex exhibits antitumour activity by inducing cell apoptosis, which results from the accumulation of large amounts of unrepaired DNA damage. SN - 1973-9478 UR - https://www.unboundmedicine.com/medline/citation/24070188/A_novel_ruthenium_II__polypyridyl_complex_inhibits_cell_proliferation_and_induces_cell_apoptosis_by_impairing_DNA_damage_repair_ L2 - https://www.tandfonline.com/doi/full/10.1179/1973947813Y.0000000138 DB - PRIME DP - Unbound Medicine ER -