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Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae).
Ultrason Sonochem. 2014 Mar; 21(2):478-84.US

Abstract

This work reports a comparative study about extraction methods used to obtain anthraquinones (AQs) from stems and leaves of Heterophyllae pustulata Hook (Rubiáceae). One of the conventional procedures used to extract these metabolites from a vegetable matrix is by successive Soxhlet extractions with solvents of increasing polarity: starting with hexane to eliminate chlorophylls and fatty components, following by benzene and finally ethyl acetate. However, this technique shows a low extraction yield of total AQs, and consumes large quantities of solvent and time. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have been investigated as alternative methods to extract these compounds, using the same sequence of solvents. It was found that UAE increases the extraction yield of total AQs and reduces the time and amount of solvent used. Nevertheless, the combination UAE with benzene, plus MAE with ethyl acetate at a constant power of 900 W showed the best results. A higher yield of total AQs was obtained in less time and using the same amount of solvent that UAE. The optimal conditions for this latter procedure were UAE with benzene at 50 °C during 60 min, followed by MAE at 900 W during 15 min using ethyl acetate as extraction solvent.

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Authors+Show Affiliations

Barrera Vázquez MF
IDTQ-Grupo Vinculado PLAPIQUI - CONICET, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 1611, Ciudad Universitaria, Córdoba, Argentina.
Comini LR
No affiliation info available
Martini RE
No affiliation info available
Núñez Montoya SC
No affiliation info available
Bottini S
No affiliation info available
Cabrera JL
No affiliation info available

MeSH

AnthraquinonesChemical FractionationMicrowavesRubiaceaeSolventsTime FactorsUltrasonics

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24071561

Citation

Barrera Vázquez, M F., et al. "Comparisons Between Conventional, Ultrasound-assisted and Microwave-assisted Methods for Extraction of Anthraquinones From Heterophyllaea Pustulata Hook F. (Rubiaceae)." Ultrasonics Sonochemistry, vol. 21, no. 2, 2014, pp. 478-84.
Barrera Vázquez MF, Comini LR, Martini RE, et al. Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae). Ultrason Sonochem. 2014;21(2):478-84.
Barrera Vázquez, M. F., Comini, L. R., Martini, R. E., Núñez Montoya, S. C., Bottini, S., & Cabrera, J. L. (2014). Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae). Ultrasonics Sonochemistry, 21(2), 478-84. https://doi.org/10.1016/j.ultsonch.2013.08.023
Barrera Vázquez MF, et al. Comparisons Between Conventional, Ultrasound-assisted and Microwave-assisted Methods for Extraction of Anthraquinones From Heterophyllaea Pustulata Hook F. (Rubiaceae). Ultrason Sonochem. 2014;21(2):478-84. PubMed PMID: 24071561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae). AU - Barrera Vázquez,M F, AU - Comini,L R, AU - Martini,R E, AU - Núñez Montoya,S C, AU - Bottini,S, AU - Cabrera,J L, Y1 - 2013/09/10/ PY - 2012/12/12/received PY - 2013/08/20/revised PY - 2013/08/31/accepted PY - 2013/9/28/entrez PY - 2013/9/28/pubmed PY - 2014/7/16/medline KW - AQs KW - AUP KW - Anthraquinones KW - Emicrowave-assisted extraction KW - HPLC KW - High-performance liquid chromatography KW - MAE KW - Microwave assisted extraction KW - UAE KW - Ultrasound-assisted extraction KW - anthraquinones KW - area under each peak KW - retention times KW - singlet molecular oxygen KW - superoxide anion KW - t(R) SP - 478 EP - 84 JF - Ultrasonics sonochemistry JO - Ultrason Sonochem VL - 21 IS - 2 N2 - This work reports a comparative study about extraction methods used to obtain anthraquinones (AQs) from stems and leaves of Heterophyllae pustulata Hook (Rubiáceae). One of the conventional procedures used to extract these metabolites from a vegetable matrix is by successive Soxhlet extractions with solvents of increasing polarity: starting with hexane to eliminate chlorophylls and fatty components, following by benzene and finally ethyl acetate. However, this technique shows a low extraction yield of total AQs, and consumes large quantities of solvent and time. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have been investigated as alternative methods to extract these compounds, using the same sequence of solvents. It was found that UAE increases the extraction yield of total AQs and reduces the time and amount of solvent used. Nevertheless, the combination UAE with benzene, plus MAE with ethyl acetate at a constant power of 900 W showed the best results. A higher yield of total AQs was obtained in less time and using the same amount of solvent that UAE. The optimal conditions for this latter procedure were UAE with benzene at 50 °C during 60 min, followed by MAE at 900 W during 15 min using ethyl acetate as extraction solvent. SN - 1873-2828 UR - https://www.unboundmedicine.com/medline/citation/24071561/Comparisons_between_conventional_ultrasound_assisted_and_microwave_assisted_methods_for_extraction_of_anthraquinones_from_Heterophyllaea_pustulata_Hook_f___Rubiaceae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1350-4177(13)00194-6 DB - PRIME DP - Unbound Medicine ER -