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Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams.
Vet Microbiol. 2013 Dec 27; 167(3-4):546-53.VM

Abstract

Ovine brucellosis caused by Brucella ovis is considered one of the most important reproductive diseases of rams worldwide. This study aimed to characterize the kinetics of infection of a ΔabcAB B. ovis mutant strain in rams. Twelve 1-year-old crossbred rams were used. Six rams were challenged with 2 mL of a suspension containing 1.2×10(9) CFU/mL of B. ovis strain ATCC25840 (wild type) by intraprepucial inoculation and additional 50 μL in each conjunctival sac of a suspension containing 1.2×10(10) CFU/mL of the same strain. The other six rams were challenged with an equivalent number of CFU of the mutant strain ΔabcAB B. ovis through the same routes. Serum samples for serology and semen and urine samples for bacteriologic culture and PCR were collected weekly during 24 weeks. At 24 weeks post infection, tissue samples were collected for bacteriologic culture and PCR. All rams inoculated with wild type or the ΔabcAB strain seroconverted at the fourth week post infection, remaining positive up to the 16th week post infection. PCR and bacteriology demonstrated that only rams inoculated with the wild type strain shed the organism in semen and urine. Lymphocytes from rams inoculated with wild type or ΔabcAB B. ovis had significantly higher proliferation in response to B. ovis antigens when compared with unstimulated controls. Tissue bacteriology and PCR detected B. ovis in all rams challenged with the wild type strain, whereas only one ΔabcAB-infected ram had a positive iliac lymph node sample by PCR.

Authors+Show Affiliations

Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24075357

Citation

Silva, Ana Patrícia C., et al. "Brucella Ovis Lacking a Species-specific Putative ATP-binding Cassette Transporter Is Attenuated but Immunogenic in Rams." Veterinary Microbiology, vol. 167, no. 3-4, 2013, pp. 546-53.
Silva AP, Macêdo AA, Costa LF, et al. Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams. Vet Microbiol. 2013;167(3-4):546-53.
Silva, A. P., Macêdo, A. A., Costa, L. F., Turchetti, A. P., Bull, V., Pessoa, M. S., Araújo, M. S., Nascimento, E. F., Martins-Filho, O. A., Paixão, T. A., & Santos, R. L. (2013). Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams. Veterinary Microbiology, 167(3-4), 546-53. https://doi.org/10.1016/j.vetmic.2013.09.003
Silva AP, et al. Brucella Ovis Lacking a Species-specific Putative ATP-binding Cassette Transporter Is Attenuated but Immunogenic in Rams. Vet Microbiol. 2013 Dec 27;167(3-4):546-53. PubMed PMID: 24075357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams. AU - Silva,Ana Patrícia C, AU - Macêdo,Auricélio A, AU - Costa,Luciana F, AU - Turchetti,Andréia P, AU - Bull,Valquíria, AU - Pessoa,Moisés S, AU - Araújo,Márcio S S, AU - Nascimento,Ernane F, AU - Martins-Filho,Olindo A, AU - Paixão,Tatiane A, AU - Santos,Renato L, Y1 - 2013/09/12/ PY - 2013/06/22/received PY - 2013/09/02/revised PY - 2013/09/04/accepted PY - 2013/10/1/entrez PY - 2013/10/1/pubmed PY - 2014/5/27/medline KW - Brucella ovis KW - Immunogenic potential KW - Kinetics KW - Rams KW - ΔabcAB SP - 546 EP - 53 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 167 IS - 3-4 N2 - Ovine brucellosis caused by Brucella ovis is considered one of the most important reproductive diseases of rams worldwide. This study aimed to characterize the kinetics of infection of a ΔabcAB B. ovis mutant strain in rams. Twelve 1-year-old crossbred rams were used. Six rams were challenged with 2 mL of a suspension containing 1.2×10(9) CFU/mL of B. ovis strain ATCC25840 (wild type) by intraprepucial inoculation and additional 50 μL in each conjunctival sac of a suspension containing 1.2×10(10) CFU/mL of the same strain. The other six rams were challenged with an equivalent number of CFU of the mutant strain ΔabcAB B. ovis through the same routes. Serum samples for serology and semen and urine samples for bacteriologic culture and PCR were collected weekly during 24 weeks. At 24 weeks post infection, tissue samples were collected for bacteriologic culture and PCR. All rams inoculated with wild type or the ΔabcAB strain seroconverted at the fourth week post infection, remaining positive up to the 16th week post infection. PCR and bacteriology demonstrated that only rams inoculated with the wild type strain shed the organism in semen and urine. Lymphocytes from rams inoculated with wild type or ΔabcAB B. ovis had significantly higher proliferation in response to B. ovis antigens when compared with unstimulated controls. Tissue bacteriology and PCR detected B. ovis in all rams challenged with the wild type strain, whereas only one ΔabcAB-infected ram had a positive iliac lymph node sample by PCR. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/24075357/Brucella_ovis_lacking_a_species_specific_putative_ATP_binding_cassette_transporter_is_attenuated_but_immunogenic_in_rams_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(13)00440-9 DB - PRIME DP - Unbound Medicine ER -