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Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography.
J Chromatogr A. 2013 Nov 01; 1314:63-9.JC

Abstract

As a naturally hydrophilic peptide, glutathione was facilely immobilized onto silica surface to obtain a novel hydrophilic interaction/cation-exchange mixed-mode chromatographic stationary phase (Click TE-GSH) via copper-free "thiol-ene" click chemistry. The resulting material was characterized by solid state (13)C/CP MAS NMR and elemental analysis. The measurement of ζ-potential indicated the cation-exchange characteristics and adjustable surface charge density of Click TE-GSH material. The influence of acetonitrile content and pH value on the retention of ionic compounds was investigated for understanding the chromatographic behaviors. The results demonstrated that Click TE-GSH column could provide both hydrophilic and cation-exchange interaction. Taking advantage of the good hydrophilicity and inherent cation-exchange characteristics of Click TE-GSH material, the resolution of neutral fructosan with high degree of polymerization (DP), basic chitooligosaccharides and strongly acidic carrageenan oligosaccharides was successfully realized in hydrophilic interaction chromatography (HILIC), hydrophilic interaction/cation-exchange mixed-mode chromatography (HILIC/CEX), cation-exchange chromatography (CEX) and electrostatic repulsion/hydrophilic interaction chromatography (ERLIC). On the other hand, the separation of standard peptides varying in hydrophobicity/hydrophilicity and charge was achieved in both CEX and HILIC/CEX mode with high efficiency and distinct selectivity. To further demonstrate the versatility and applicability of Click TE-GSH stationary phase, the separation of a human serum albumin (HSA) tryptic digest was performed in HILIC/CEX mode. Peptides were adequately resolved and up to 86 HSA peptides were identified with sequence coverage of 85%. The results indicated the good potential of Click TE-GSH material in glycomics and proteomics.

Authors+Show Affiliations

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24075460

Citation

Shen, Aijin, et al. "Glutathione-based Zwitterionic Stationary Phase for Hydrophilic Interaction/cation-exchange Mixed-mode Chromatography." Journal of Chromatography. A, vol. 1314, 2013, pp. 63-9.
Shen A, Li X, Dong X, et al. Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography. J Chromatogr A. 2013;1314:63-9.
Shen, A., Li, X., Dong, X., Wei, J., Guo, Z., & Liang, X. (2013). Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography. Journal of Chromatography. A, 1314, 63-9. https://doi.org/10.1016/j.chroma.2013.09.002
Shen A, et al. Glutathione-based Zwitterionic Stationary Phase for Hydrophilic Interaction/cation-exchange Mixed-mode Chromatography. J Chromatogr A. 2013 Nov 1;1314:63-9. PubMed PMID: 24075460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography. AU - Shen,Aijin, AU - Li,Xiuling, AU - Dong,Xuefang, AU - Wei,Jie, AU - Guo,Zhimou, AU - Liang,Xinmiao, Y1 - 2013/09/08/ PY - 2013/07/24/received PY - 2013/08/29/revised PY - 2013/09/01/accepted PY - 2013/10/1/entrez PY - 2013/10/1/pubmed PY - 2014/2/5/medline KW - Glutathione KW - Hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) KW - Mixed-mode KW - Thiol-ene KW - Zwitterionic stationary phase SP - 63 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1314 N2 - As a naturally hydrophilic peptide, glutathione was facilely immobilized onto silica surface to obtain a novel hydrophilic interaction/cation-exchange mixed-mode chromatographic stationary phase (Click TE-GSH) via copper-free "thiol-ene" click chemistry. The resulting material was characterized by solid state (13)C/CP MAS NMR and elemental analysis. The measurement of ζ-potential indicated the cation-exchange characteristics and adjustable surface charge density of Click TE-GSH material. The influence of acetonitrile content and pH value on the retention of ionic compounds was investigated for understanding the chromatographic behaviors. The results demonstrated that Click TE-GSH column could provide both hydrophilic and cation-exchange interaction. Taking advantage of the good hydrophilicity and inherent cation-exchange characteristics of Click TE-GSH material, the resolution of neutral fructosan with high degree of polymerization (DP), basic chitooligosaccharides and strongly acidic carrageenan oligosaccharides was successfully realized in hydrophilic interaction chromatography (HILIC), hydrophilic interaction/cation-exchange mixed-mode chromatography (HILIC/CEX), cation-exchange chromatography (CEX) and electrostatic repulsion/hydrophilic interaction chromatography (ERLIC). On the other hand, the separation of standard peptides varying in hydrophobicity/hydrophilicity and charge was achieved in both CEX and HILIC/CEX mode with high efficiency and distinct selectivity. To further demonstrate the versatility and applicability of Click TE-GSH stationary phase, the separation of a human serum albumin (HSA) tryptic digest was performed in HILIC/CEX mode. Peptides were adequately resolved and up to 86 HSA peptides were identified with sequence coverage of 85%. The results indicated the good potential of Click TE-GSH material in glycomics and proteomics. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/24075460/Glutathione_based_zwitterionic_stationary_phase_for_hydrophilic_interaction/cation_exchange_mixed_mode_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(13)01408-8 DB - PRIME DP - Unbound Medicine ER -