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Waterborne cadmium impacts immunocytotoxic and cytogenotoxic endpoints in green-lipped mussel, Perna canaliculus.
Aquat Toxicol. 2013 Oct 15; 142-143:283-93.AT

Abstract

Mussels are sentinel species that can be used to monitor coastal metal pollution through the application of biomarkers. Among the several important metal toxicants in coastal settings, cadmium (Cd) is of particular concern, being a non-essential metal that is known to cause harmful impacts in aquatic organisms at low concentrations. The aim of the present study was to examine the immunocytotoxic and cytogenotoxic effects of Cd on the green-lipped mussel, Perna canaliculus, under laboratory conditions. The acute (96 h; 0, 2000 and 4000 μg Cd L(-1)) and subchronic (28 d; 0, 200 and 2000 μg Cd L(-1)) toxic effects of waterborne Cd were measured in haemocytes and gill cells using differential haemocyte cell count, the micronucleus test and the comet assay. During subchronic exposure to Cd the relative counts of eosinophils and hyalinocytes increased significantly in Cd-exposed mussels while the proportion of basophils decreased. All of these effects were time- and concentration-dependent. Conversely, the relative numbers of basophils and eosinophils increased significantly during acute Cd exposure. Nuclear aberrations such as the formation of micronuclei, nuclear buds, fragmented-apoptotic cells and binuclei were observed in gill cells of Cd-exposed mussels. All of these parameters increased significantly at 2000 μg Cd L(-1) during subchronic exposure to Cd, and all showed a strong and significant correlation to gill Cd accumulation. Comet assay results demonstrated a significant increase in DNA damage in the haemocytes of mussels exposed to subchronic Cd concentrations. The results indicate that Cd has the capacity to induce immune system and genotoxic damage in green-lipped mussels, an impact that may have implications such as reduced disease resistance and compromised survival. These data also suggest that immunocytotoxic and cytogenotoxic biomarkers would be a valuable addition to environmental monitoring programmes using green-lipped mussels.

Authors+Show Affiliations

School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand. Electronic address: rch118@uclive.ac.nz.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24077184

Citation

Chandurvelan, Rathishri, et al. "Waterborne Cadmium Impacts Immunocytotoxic and Cytogenotoxic Endpoints in Green-lipped Mussel, Perna Canaliculus." Aquatic Toxicology (Amsterdam, Netherlands), vol. 142-143, 2013, pp. 283-93.
Chandurvelan R, Marsden ID, Gaw S, et al. Waterborne cadmium impacts immunocytotoxic and cytogenotoxic endpoints in green-lipped mussel, Perna canaliculus. Aquat Toxicol. 2013;142-143:283-93.
Chandurvelan, R., Marsden, I. D., Gaw, S., & Glover, C. N. (2013). Waterborne cadmium impacts immunocytotoxic and cytogenotoxic endpoints in green-lipped mussel, Perna canaliculus. Aquatic Toxicology (Amsterdam, Netherlands), 142-143, 283-93. https://doi.org/10.1016/j.aquatox.2013.09.002
Chandurvelan R, et al. Waterborne Cadmium Impacts Immunocytotoxic and Cytogenotoxic Endpoints in Green-lipped Mussel, Perna Canaliculus. Aquat Toxicol. 2013 Oct 15;142-143:283-93. PubMed PMID: 24077184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Waterborne cadmium impacts immunocytotoxic and cytogenotoxic endpoints in green-lipped mussel, Perna canaliculus. AU - Chandurvelan,Rathishri, AU - Marsden,Islay D, AU - Gaw,Sally, AU - Glover,Chris N, Y1 - 2013/09/12/ PY - 2013/05/23/received PY - 2013/09/02/revised PY - 2013/09/03/accepted PY - 2013/10/1/entrez PY - 2013/10/1/pubmed PY - 2014/3/22/medline KW - Comet assay KW - Genotoxic KW - Gill KW - Haemocytes KW - Immune system KW - Micronucleus test SP - 283 EP - 93 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 142-143 N2 - Mussels are sentinel species that can be used to monitor coastal metal pollution through the application of biomarkers. Among the several important metal toxicants in coastal settings, cadmium (Cd) is of particular concern, being a non-essential metal that is known to cause harmful impacts in aquatic organisms at low concentrations. The aim of the present study was to examine the immunocytotoxic and cytogenotoxic effects of Cd on the green-lipped mussel, Perna canaliculus, under laboratory conditions. The acute (96 h; 0, 2000 and 4000 μg Cd L(-1)) and subchronic (28 d; 0, 200 and 2000 μg Cd L(-1)) toxic effects of waterborne Cd were measured in haemocytes and gill cells using differential haemocyte cell count, the micronucleus test and the comet assay. During subchronic exposure to Cd the relative counts of eosinophils and hyalinocytes increased significantly in Cd-exposed mussels while the proportion of basophils decreased. All of these effects were time- and concentration-dependent. Conversely, the relative numbers of basophils and eosinophils increased significantly during acute Cd exposure. Nuclear aberrations such as the formation of micronuclei, nuclear buds, fragmented-apoptotic cells and binuclei were observed in gill cells of Cd-exposed mussels. All of these parameters increased significantly at 2000 μg Cd L(-1) during subchronic exposure to Cd, and all showed a strong and significant correlation to gill Cd accumulation. Comet assay results demonstrated a significant increase in DNA damage in the haemocytes of mussels exposed to subchronic Cd concentrations. The results indicate that Cd has the capacity to induce immune system and genotoxic damage in green-lipped mussels, an impact that may have implications such as reduced disease resistance and compromised survival. These data also suggest that immunocytotoxic and cytogenotoxic biomarkers would be a valuable addition to environmental monitoring programmes using green-lipped mussels. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/24077184/Waterborne_cadmium_impacts_immunocytotoxic_and_cytogenotoxic_endpoints_in_green_lipped_mussel_Perna_canaliculus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(13)00229-4 DB - PRIME DP - Unbound Medicine ER -