Abstract
BACKGROUND
Radiofrequency (RF) ablation to treat atrial arrhythmia is limited by the inability to reliably assess lesion durability and transmurality.
OBJECTIVE
The purpose of this study was to determine the feasibility of photoacoustic characterization of myocardial ablation lesions in vitro. In this study, we investigated the feasibility of combined ultrasound (US) and spectroscopic photoacoustic imaging to visualize RF ablation lesions in three dimensions (3D) based on unique differences in the optical absorption spectra between normal and ablated myocardial tissue.
METHODS
Tissue samples were excised from the ventricles of fresh porcine hearts. Lesions were generated using an RF catheter ablation system using 20 to 30 W of power applied for 40 to 60 seconds. Ablated samples were imaged in the near-infrared regime (740-780 nm) using a combined PA/US imaging system. Measured PA spectra were correlated to the absorption spectra of deoxyhemoglobin and ablated tissue to produce a tissue characterization map (TCM) identifying 3D lesion location and extent. Tissue samples were stained and photographed for gross pathology. TCM and gross pathology images were coregistered to assess TCM accuracy.
RESULTS
TCM reliably characterized ablated and non-ablated tissue up to depths of 3 mm. TCM also assessed lesion position and extent with submillimeter accuracy in multiple dimensions. Segmented TCMs achieved >69% agreement with gross pathology.
CONCLUSION
The study results suggest that spectroscopic photoacoustic imaging has the potential to accurately assess RF ablation lesion size and position with submillimeter precision and may be well suited to guide transcatheter RF atrial ablation in clinical practice.
TY - JOUR
T1 - In vitro photoacoustic visualization of myocardial ablation lesions.
AU - Dana,Nicholas,
AU - Di Biase,Luigi,
AU - Natale,Andrea,
AU - Emelianov,Stanislav,
AU - Bouchard,Richard,
Y1 - 2013/09/27/
PY - 2013/08/25/received
PY - 2013/10/2/entrez
PY - 2013/10/2/pubmed
PY - 2016/4/26/medline
KW - 3D
KW - AF
KW - ARFI
KW - Ablation
KW - Atrial fibrillation
KW - CBR
KW - FOV
KW - Hb
KW - Imaging
KW - MRI
KW - NTB
KW - OCT
KW - PA
KW - PBS
KW - Photoacoustic
KW - RF
KW - ROI
KW - RT
KW - Radiofrequency
KW - TCM
KW - Tissue characterization
KW - US
KW - acoustic radiation force impulse
KW - atrial fibrillation
KW - contrast-to-background ratio
KW - deoxyhemoglobin
KW - field of view
KW - magnetic resonance imaging
KW - nitrotetrazolium blue
KW - optical coherence tomography
KW - phosphate-buffered saline
KW - photoacoustic
KW - radiofrequency
KW - real time
KW - region of interest
KW - sPA
KW - spectroscopic photoacoustic
KW - tPA
KW - thermographic photoacoustic
KW - three-dimensional
KW - tissue characterization map
KW - ultrasound
SP - 150
EP - 7
JF - Heart rhythm
JO - Heart Rhythm
VL - 11
IS - 1
N2 - BACKGROUND: Radiofrequency (RF) ablation to treat atrial arrhythmia is limited by the inability to reliably assess lesion durability and transmurality. OBJECTIVE: The purpose of this study was to determine the feasibility of photoacoustic characterization of myocardial ablation lesions in vitro. In this study, we investigated the feasibility of combined ultrasound (US) and spectroscopic photoacoustic imaging to visualize RF ablation lesions in three dimensions (3D) based on unique differences in the optical absorption spectra between normal and ablated myocardial tissue. METHODS: Tissue samples were excised from the ventricles of fresh porcine hearts. Lesions were generated using an RF catheter ablation system using 20 to 30 W of power applied for 40 to 60 seconds. Ablated samples were imaged in the near-infrared regime (740-780 nm) using a combined PA/US imaging system. Measured PA spectra were correlated to the absorption spectra of deoxyhemoglobin and ablated tissue to produce a tissue characterization map (TCM) identifying 3D lesion location and extent. Tissue samples were stained and photographed for gross pathology. TCM and gross pathology images were coregistered to assess TCM accuracy. RESULTS: TCM reliably characterized ablated and non-ablated tissue up to depths of 3 mm. TCM also assessed lesion position and extent with submillimeter accuracy in multiple dimensions. Segmented TCMs achieved >69% agreement with gross pathology. CONCLUSION: The study results suggest that spectroscopic photoacoustic imaging has the potential to accurately assess RF ablation lesion size and position with submillimeter precision and may be well suited to guide transcatheter RF atrial ablation in clinical practice.
SN - 1556-3871
UR - https://www.unboundmedicine.com/medline/citation/24080065/In_vitro_photoacoustic_visualization_of_myocardial_ablation_lesions_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S1547-5271(13)01082-5
DB - PRIME
DP - Unbound Medicine
ER -