Tags

Type your tag names separated by a space and hit enter

Isolation and molecular analysis of genes Stpk-V2 and Stpk-V3 homologous to powdery mildew resistance gene Stpk-V in a Dasypyrum villosum accession and its derivatives.
J Appl Genet. 2013 Nov; 54(4):417-26.JA

Abstract

Wheat-Dasypyrum villosum translocated chromosomes T6V#2S•6AL and T6V#4S•6DL are known to confer excellent resistance to wheat powdery mildew (PM). However, it is difficult to distinguish the two sources of PM resistance genes through multi-pathotype testing because to date no virulence for them has been found. To reveal the relationship between the PM resistance genes from the two translocations, the sequence of the Stpk-V gene, a key member of powdery mildew resistance locus Pm21, was used as a reference to isolate homologous genes from a D. villosum accession No.1026 and its derivatives 6V#4(6D) disomic substitution (DS) line RW15 and T6V#4S•6DL translocation line Pm97033. Two genes Stpk-V2 and Stpk-V3 were cloned from No.1026. Sequence alignment showed that Stpk-V2 and Stpk-V3 shared 98.2 % and 96.2 % of their DNA and 99.3 % and 100 % of their amino acids in identity with Stpk-V. Compared with Stpk-V, a 22-bp direct sequence repeat and a miniature inverted-repeat transposable element (MITE) were found in the intron 4 of Stpk-V2 and Stpk-V3, respectively. However, Stpk-V2 was not present in DS line RW15 and translocation line Pm97033 based on the PCR result, indicating that Stpk-V2 did not contribute to the PM resistance of RW15 and Pm97033. In the promoter region, a 78-bp insertion was found not only in Stpk-V2 and Stpk-V3, but also in its orthologous gene Stpk-A of wheat. In addition, there was a 17 bp/8 bp deletion/insertion in the putative promoter of Stpk-V3 in comparison with that of Stpk-V/Stpk-V2. Real-time quantitative RT-PCR analysis indicated that the expression levels of Stpk-V and Stpk-V3 genes in the translocation lines were induced by the pathogen, but Stpk-V had a higher expression level than Stpk-V3 at 12 h after inoculation with Bgt. The diversity of Stpk-V gene will help to explore new resistance genes to PM in D. villosum for wheat breeding.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Lin ZS
National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Zhang YL
No affiliation info available
Wang MJ
No affiliation info available
Li JR
No affiliation info available
Wang K
No affiliation info available
Chen X
No affiliation info available
Xu QF
No affiliation info available
Zhang XS
No affiliation info available
Ye XG
No affiliation info available

MeSH

Amino Acid SequenceAscomycotaBase SequenceDNA, PlantDisease ResistanceGenes, PlantMolecular Sequence DataPlant DiseasesPoaceaePromoter Regions, GeneticRNA, PlantSequence Analysis, DNATranslocation, Genetic

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24081822

Citation

Lin, Z S., et al. "Isolation and Molecular Analysis of Genes Stpk-V2 and Stpk-V3 Homologous to Powdery Mildew Resistance Gene Stpk-V in a Dasypyrum Villosum Accession and Its Derivatives." Journal of Applied Genetics, vol. 54, no. 4, 2013, pp. 417-26.
Lin ZS, Zhang YL, Wang MJ, et al. Isolation and molecular analysis of genes Stpk-V2 and Stpk-V3 homologous to powdery mildew resistance gene Stpk-V in a Dasypyrum villosum accession and its derivatives. J Appl Genet. 2013;54(4):417-26.
Lin, Z. S., Zhang, Y. L., Wang, M. J., Li, J. R., Wang, K., Chen, X., Xu, Q. F., Zhang, X. S., & Ye, X. G. (2013). Isolation and molecular analysis of genes Stpk-V2 and Stpk-V3 homologous to powdery mildew resistance gene Stpk-V in a Dasypyrum villosum accession and its derivatives. Journal of Applied Genetics, 54(4), 417-26. https://doi.org/10.1007/s13353-013-0172-y
Lin ZS, et al. Isolation and Molecular Analysis of Genes Stpk-V2 and Stpk-V3 Homologous to Powdery Mildew Resistance Gene Stpk-V in a Dasypyrum Villosum Accession and Its Derivatives. J Appl Genet. 2013;54(4):417-26. PubMed PMID: 24081822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and molecular analysis of genes Stpk-V2 and Stpk-V3 homologous to powdery mildew resistance gene Stpk-V in a Dasypyrum villosum accession and its derivatives. AU - Lin,Z S, AU - Zhang,Y L, AU - Wang,M J, AU - Li,J R, AU - Wang,K, AU - Chen,X, AU - Xu,Q F, AU - Zhang,X S, AU - Ye,X G, Y1 - 2013/10/01/ PY - 2013/03/12/received PY - 2013/09/09/accepted PY - 2013/09/08/revised PY - 2013/10/2/entrez PY - 2013/10/2/pubmed PY - 2014/7/10/medline SP - 417 EP - 26 JF - Journal of applied genetics JO - J Appl Genet VL - 54 IS - 4 N2 - Wheat-Dasypyrum villosum translocated chromosomes T6V#2S•6AL and T6V#4S•6DL are known to confer excellent resistance to wheat powdery mildew (PM). However, it is difficult to distinguish the two sources of PM resistance genes through multi-pathotype testing because to date no virulence for them has been found. To reveal the relationship between the PM resistance genes from the two translocations, the sequence of the Stpk-V gene, a key member of powdery mildew resistance locus Pm21, was used as a reference to isolate homologous genes from a D. villosum accession No.1026 and its derivatives 6V#4(6D) disomic substitution (DS) line RW15 and T6V#4S•6DL translocation line Pm97033. Two genes Stpk-V2 and Stpk-V3 were cloned from No.1026. Sequence alignment showed that Stpk-V2 and Stpk-V3 shared 98.2 % and 96.2 % of their DNA and 99.3 % and 100 % of their amino acids in identity with Stpk-V. Compared with Stpk-V, a 22-bp direct sequence repeat and a miniature inverted-repeat transposable element (MITE) were found in the intron 4 of Stpk-V2 and Stpk-V3, respectively. However, Stpk-V2 was not present in DS line RW15 and translocation line Pm97033 based on the PCR result, indicating that Stpk-V2 did not contribute to the PM resistance of RW15 and Pm97033. In the promoter region, a 78-bp insertion was found not only in Stpk-V2 and Stpk-V3, but also in its orthologous gene Stpk-A of wheat. In addition, there was a 17 bp/8 bp deletion/insertion in the putative promoter of Stpk-V3 in comparison with that of Stpk-V/Stpk-V2. Real-time quantitative RT-PCR analysis indicated that the expression levels of Stpk-V and Stpk-V3 genes in the translocation lines were induced by the pathogen, but Stpk-V had a higher expression level than Stpk-V3 at 12 h after inoculation with Bgt. The diversity of Stpk-V gene will help to explore new resistance genes to PM in D. villosum for wheat breeding. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/24081822/Isolation_and_molecular_analysis_of_genes_Stpk_V2_and_Stpk_V3_homologous_to_powdery_mildew_resistance_gene_Stpk_V_in_a_Dasypyrum_villosum_accession_and_its_derivatives_ L2 - https://dx.doi.org/10.1007/s13353-013-0172-y DB - PRIME DP - Unbound Medicine ER -