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Accurate cleavage and polyadenylation of exogenous RNA substrate.
Cell. 1985 Jul; 41(3):845-55.Cell

Abstract

Purified precursor RNA containing the L3 polyadenylation site of late adenovirus 2 mRNA is accurately cleaved and polyadenylated when incubated with nuclear extract from HeLa cells. The reaction is very efficient; 75% of the precursor is correctly processed. Cleavage is rapidly followed by polymerization of an initial poly(A) tract of approximately 130 nucleotides. Additional adenosine residues are added during further incubation. In the presence of the ATP analog alpha-beta-methylene-adenosine 5' triphosphate, the precursor RNA is cleaved but not polyadenylated, suggesting that processing is not coupled to the synthesis of the initial poly(A) tract. In the absence of free Mg2+, a small RNA of approximately 46 nucleotides is stabilized against degradation. Fingerprint analysis suggests this RNA is produced by endonucleolytic cleavage at the L3 site. Like the in vitro splicing reaction, the in vitro polyadenylation reaction is inhibited by adding antiserum against the small nuclear ribonucleoprotein particle containing U1 RNA.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2408761

Citation

Moore, C L., and P A. Sharp. "Accurate Cleavage and Polyadenylation of Exogenous RNA Substrate." Cell, vol. 41, no. 3, 1985, pp. 845-55.
Moore CL, Sharp PA. Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell. 1985;41(3):845-55.
Moore, C. L., & Sharp, P. A. (1985). Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell, 41(3), 845-55.
Moore CL, Sharp PA. Accurate Cleavage and Polyadenylation of Exogenous RNA Substrate. Cell. 1985;41(3):845-55. PubMed PMID: 2408761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accurate cleavage and polyadenylation of exogenous RNA substrate. AU - Moore,C L, AU - Sharp,P A, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 845 EP - 55 JF - Cell JO - Cell VL - 41 IS - 3 N2 - Purified precursor RNA containing the L3 polyadenylation site of late adenovirus 2 mRNA is accurately cleaved and polyadenylated when incubated with nuclear extract from HeLa cells. The reaction is very efficient; 75% of the precursor is correctly processed. Cleavage is rapidly followed by polymerization of an initial poly(A) tract of approximately 130 nucleotides. Additional adenosine residues are added during further incubation. In the presence of the ATP analog alpha-beta-methylene-adenosine 5' triphosphate, the precursor RNA is cleaved but not polyadenylated, suggesting that processing is not coupled to the synthesis of the initial poly(A) tract. In the absence of free Mg2+, a small RNA of approximately 46 nucleotides is stabilized against degradation. Fingerprint analysis suggests this RNA is produced by endonucleolytic cleavage at the L3 site. Like the in vitro splicing reaction, the in vitro polyadenylation reaction is inhibited by adding antiserum against the small nuclear ribonucleoprotein particle containing U1 RNA. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/2408761/Accurate_cleavage_and_polyadenylation_of_exogenous_RNA_substrate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(85)80065-9 DB - PRIME DP - Unbound Medicine ER -