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Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells.
Respir Res. 2013 Oct 02; 14:97.RR

Abstract

BACKGROUND

Cigarette smoking is the major risk factor for COPD, leading to chronic airway inflammation. We hypothesized that cigarette smoke induces structural and functional changes of airway epithelial mitochondria, with important implications for lung inflammation and COPD pathogenesis.

METHODS

We studied changes in mitochondrial morphology and in expression of markers for mitochondrial capacity, damage/biogenesis and fission/fusion in the human bronchial epithelial cell line BEAS-2B upon 6-months from ex-smoking COPD GOLD stage IV patients to age-matched smoking and never-smoking controls.

RESULTS

We observed that long-term CSE exposure induces robust changes in mitochondrial structure, including fragmentation, branching and quantity of cristae. The majority of these changes were persistent upon CSE depletion. Furthermore, long-term CSE exposure significantly increased the expression of specific fission/fusion markers (Fis1, Mfn1, Mfn2, Drp1 and Opa1), oxidative phosphorylation (OXPHOS) proteins (Complex II, III and V), and oxidative stress (Mn-SOD) markers. These changes were accompanied by increased levels of the pro-inflammatory mediators IL-6, IL-8, and IL-1β. Importantly, COPD primary bronchial epithelial cells (PBECs) displayed similar changes in mitochondrial morphology as observed in long-term CSE-exposure BEAS-2B cells. Moreover, expression of specific OXPHOS proteins was higher in PBECs from COPD patients than control smokers, as was the expression of mitochondrial stress marker PINK1.

CONCLUSION

The observed mitochondrial changes in COPD epithelium are potentially the consequence of long-term exposure to cigarette smoke, leading to impaired mitochondrial function and may play a role in the pathogenesis of COPD.

Authors+Show Affiliations

Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. r.f.hoffmann@umcg.nl.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24088173

Citation

Hoffmann, Roland F., et al. "Prolonged Cigarette Smoke Exposure Alters Mitochondrial Structure and Function in Airway Epithelial Cells." Respiratory Research, vol. 14, 2013, p. 97.
Hoffmann RF, Zarrintan S, Brandenburg SM, et al. Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells. Respir Res. 2013;14:97.
Hoffmann, R. F., Zarrintan, S., Brandenburg, S. M., Kol, A., de Bruin, H. G., Jafari, S., Dijk, F., Kalicharan, D., Kelders, M., Gosker, H. R., Ten Hacken, N. H., van der Want, J. J., van Oosterhout, A. J., & Heijink, I. H. (2013). Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells. Respiratory Research, 14, 97. https://doi.org/10.1186/1465-9921-14-97
Hoffmann RF, et al. Prolonged Cigarette Smoke Exposure Alters Mitochondrial Structure and Function in Airway Epithelial Cells. Respir Res. 2013 Oct 2;14:97. PubMed PMID: 24088173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells. AU - Hoffmann,Roland F, AU - Zarrintan,Sina, AU - Brandenburg,Simone M, AU - Kol,Arjan, AU - de Bruin,Harold G, AU - Jafari,Shabnam, AU - Dijk,Freark, AU - Kalicharan,Dharamdajal, AU - Kelders,Marco, AU - Gosker,Harry R, AU - Ten Hacken,Nick Ht, AU - van der Want,Johannes J, AU - van Oosterhout,Antoon Jm, AU - Heijink,Irene H, Y1 - 2013/10/02/ PY - 2013/07/10/received PY - 2013/09/26/accepted PY - 2013/10/4/entrez PY - 2013/10/4/pubmed PY - 2014/7/9/medline SP - 97 EP - 97 JF - Respiratory research JO - Respir Res VL - 14 N2 - BACKGROUND: Cigarette smoking is the major risk factor for COPD, leading to chronic airway inflammation. We hypothesized that cigarette smoke induces structural and functional changes of airway epithelial mitochondria, with important implications for lung inflammation and COPD pathogenesis. METHODS: We studied changes in mitochondrial morphology and in expression of markers for mitochondrial capacity, damage/biogenesis and fission/fusion in the human bronchial epithelial cell line BEAS-2B upon 6-months from ex-smoking COPD GOLD stage IV patients to age-matched smoking and never-smoking controls. RESULTS: We observed that long-term CSE exposure induces robust changes in mitochondrial structure, including fragmentation, branching and quantity of cristae. The majority of these changes were persistent upon CSE depletion. Furthermore, long-term CSE exposure significantly increased the expression of specific fission/fusion markers (Fis1, Mfn1, Mfn2, Drp1 and Opa1), oxidative phosphorylation (OXPHOS) proteins (Complex II, III and V), and oxidative stress (Mn-SOD) markers. These changes were accompanied by increased levels of the pro-inflammatory mediators IL-6, IL-8, and IL-1β. Importantly, COPD primary bronchial epithelial cells (PBECs) displayed similar changes in mitochondrial morphology as observed in long-term CSE-exposure BEAS-2B cells. Moreover, expression of specific OXPHOS proteins was higher in PBECs from COPD patients than control smokers, as was the expression of mitochondrial stress marker PINK1. CONCLUSION: The observed mitochondrial changes in COPD epithelium are potentially the consequence of long-term exposure to cigarette smoke, leading to impaired mitochondrial function and may play a role in the pathogenesis of COPD. SN - 1465-993X UR - https://www.unboundmedicine.com/medline/citation/24088173/Prolonged_cigarette_smoke_exposure_alters_mitochondrial_structure_and_function_in_airway_epithelial_cells_ L2 - https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-97 DB - PRIME DP - Unbound Medicine ER -