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Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients.

Abstract

We investigated whether or not Omega-3 long-chain polyunsaturated fatty acid (omega-3 LCPUFA) supplementation exacerbates oxidative stress in homozygous sickle cell patients aged 2 to 14 years. Depending on their age, they received between one and three omega-3 (277.8mg DHA and 39.0mg EPA/capsule) or placebo (high oleic acid sunflower seed oil) capsules for one year. Supplementation increased significantly the levels of the two fatty acids in red cell phosphatidylcholine and phosphatidylethanolamine (p<0.001). The patients who received omega-3 LCPUFA compared with their placebo-taking counterparts had a higher concentration of plasma vitamin E at one year (14.3±2.8 versus 12.3±2.8µmol/l; p<0.001). The two groups had comparable concentrations of the vitamin at six month intervention (10.8±2.2 versus 10.7±2.9µmol/l; p>0.05) and baseline (10.7±3.1 versus 10.7±2.8µmol/l; p>0.05). After six month of intervention, the patients on omega 3 fatty acids had lower GPx-1 (33.5±13.4 versus 46.6 ±17.6, p<0.01) and Cu/Zn-SOD (1070±600 versus 1470±690 p<0.05) activities than at baseline. GPx-1 (33.5±17.6IU/g Hb versus 43.7±13.2IU/g Hb; p<0.01) and Cu/Zn-SOD (1070±600IU/g Hb versus 1360±920IU/g Hb; p>0.05) activities were reduced in the omega 3 compared with the placebo at six month intervention. There was no difference in the activity of either of the enzymes between baseline and six month intervention in the placebo group (p>0.05). This study demonstrates; DHA and EPA supplementation, rather than exacerbating the inherent oxidative stress associated with the disease, seems to provide an antioxidant protection. Hence, it will be safe to provide omega-3 LCPUFA to sickle cell patients to help ameliorate vaso-occlusive and haemolytic crises and membrane fatty acid abnormality.

Authors+Show Affiliations

Lipidomics and Nutrition Research Centre, Faculty of Life Sciences and Computing, London Metropolitan University, 166-220 Holloway Road, London N7 8DB UK; Faculty of Medicine, University of Khartoum, Khartoum, Sudan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24095588

Citation

Daak, Ahmed A., et al. "Docosahexaenoic and Eicosapentaenoic Acid Supplementation Does Not Exacerbate Oxidative Stress or Intravascular Haemolysis in Homozygous Sickle Cell Patients." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 89, no. 5, 2013, pp. 305-11.
Daak AA, Ghebremeskel K, Mariniello K, et al. Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. Prostaglandins Leukot Essent Fatty Acids. 2013;89(5):305-11.
Daak, A. A., Ghebremeskel, K., Mariniello, K., Attallah, B., Clough, P., & Elbashir, M. I. (2013). Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 89(5), pp. 305-11. doi:10.1016/j.plefa.2013.09.006.
Daak AA, et al. Docosahexaenoic and Eicosapentaenoic Acid Supplementation Does Not Exacerbate Oxidative Stress or Intravascular Haemolysis in Homozygous Sickle Cell Patients. Prostaglandins Leukot Essent Fatty Acids. 2013;89(5):305-11. PubMed PMID: 24095588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. AU - Daak,Ahmed A, AU - Ghebremeskel,Kebreab, AU - Mariniello,Katia, AU - Attallah,Bakhita, AU - Clough,Peter, AU - Elbashir,Mustafa I, Y1 - 2013/09/18/ PY - 2013/06/05/received PY - 2013/09/05/revised PY - 2013/09/09/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/6/4/medline KW - Antioxidant enzymes KW - Omega-3 fatty acids KW - Oxidative stress KW - Sickle cell disease KW - Vitamin E SP - 305 EP - 11 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 89 IS - 5 N2 - We investigated whether or not Omega-3 long-chain polyunsaturated fatty acid (omega-3 LCPUFA) supplementation exacerbates oxidative stress in homozygous sickle cell patients aged 2 to 14 years. Depending on their age, they received between one and three omega-3 (277.8mg DHA and 39.0mg EPA/capsule) or placebo (high oleic acid sunflower seed oil) capsules for one year. Supplementation increased significantly the levels of the two fatty acids in red cell phosphatidylcholine and phosphatidylethanolamine (p<0.001). The patients who received omega-3 LCPUFA compared with their placebo-taking counterparts had a higher concentration of plasma vitamin E at one year (14.3±2.8 versus 12.3±2.8µmol/l; p<0.001). The two groups had comparable concentrations of the vitamin at six month intervention (10.8±2.2 versus 10.7±2.9µmol/l; p>0.05) and baseline (10.7±3.1 versus 10.7±2.8µmol/l; p>0.05). After six month of intervention, the patients on omega 3 fatty acids had lower GPx-1 (33.5±13.4 versus 46.6 ±17.6, p<0.01) and Cu/Zn-SOD (1070±600 versus 1470±690 p<0.05) activities than at baseline. GPx-1 (33.5±17.6IU/g Hb versus 43.7±13.2IU/g Hb; p<0.01) and Cu/Zn-SOD (1070±600IU/g Hb versus 1360±920IU/g Hb; p>0.05) activities were reduced in the omega 3 compared with the placebo at six month intervention. There was no difference in the activity of either of the enzymes between baseline and six month intervention in the placebo group (p>0.05). This study demonstrates; DHA and EPA supplementation, rather than exacerbating the inherent oxidative stress associated with the disease, seems to provide an antioxidant protection. Hence, it will be safe to provide omega-3 LCPUFA to sickle cell patients to help ameliorate vaso-occlusive and haemolytic crises and membrane fatty acid abnormality. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/24095588/Docosahexaenoic_and_eicosapentaenoic_acid_supplementation_does_not_exacerbate_oxidative_stress_or_intravascular_haemolysis_in_homozygous_sickle_cell_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(13)00185-3 DB - PRIME DP - Unbound Medicine ER -