Tags

Type your tag names separated by a space and hit enter

Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods.
Int J Infect Dis 2013; 17(12):e1134-40IJ

Abstract

OBJECTIVES

The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples.

METHODS

A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex™ II), multiplex PCR (Seeplex®), strand displacement amplification (SDA, BD ProbeTec™ ET), PCR (AmpliSens®), and a commercially available Mycoplasma IST 2 Kit.

RESULTS

Multiplex real-time PCR (Anyplex™ II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum.

CONCLUSIONS

Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms.

Authors+Show Affiliations

Department of Urology, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24095619

Citation

Choe, Hyun-Sop, et al. "Performance of Anyplex™ II Multiplex Real-time PCR for the Diagnosis of Seven Sexually Transmitted Infections: Comparison With Currently Available Methods." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 17, no. 12, 2013, pp. e1134-40.
Choe HS, Lee DS, Lee SJ, et al. Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. Int J Infect Dis. 2013;17(12):e1134-40.
Choe, H. S., Lee, D. S., Lee, S. J., Hong, S. H., Park, D. C., Lee, M. K., ... Cho, Y. H. (2013). Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 17(12), pp. e1134-40. doi:10.1016/j.ijid.2013.07.011.
Choe HS, et al. Performance of Anyplex™ II Multiplex Real-time PCR for the Diagnosis of Seven Sexually Transmitted Infections: Comparison With Currently Available Methods. Int J Infect Dis. 2013;17(12):e1134-40. PubMed PMID: 24095619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. AU - Choe,Hyun-Sop, AU - Lee,Dong Sup, AU - Lee,Seung-Ju, AU - Hong,Sung-Hoo, AU - Park,Dong Choon, AU - Lee,Mi-Kyung, AU - Kim,Tae-Hyoung, AU - Cho,Yong-Hyun, Y1 - 2013/09/07/ PY - 2013/05/24/received PY - 2013/06/23/revised PY - 2013/07/11/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/8/1/medline KW - Bacterial and parasite infection KW - Diagnosis KW - Multiplex real-time PCR KW - Sexually transmitted infection SP - e1134 EP - 40 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int. J. Infect. Dis. VL - 17 IS - 12 N2 - OBJECTIVES: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. METHODS: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex™ II), multiplex PCR (Seeplex®), strand displacement amplification (SDA, BD ProbeTec™ ET), PCR (AmpliSens®), and a commercially available Mycoplasma IST 2 Kit. RESULTS: Multiplex real-time PCR (Anyplex™ II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. CONCLUSIONS: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/24095619/Performance_of_Anyplex™_II_multiplex_real_time_PCR_for_the_diagnosis_of_seven_sexually_transmitted_infections:_comparison_with_currently_available_methods_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S1201-9712(13)00246-4 DB - PRIME DP - Unbound Medicine ER -