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Quantitative real-time PCR and phase specific serology are mutually supportive in Q fever diagnostics in goats.
Vet Microbiol. 2013 Dec 27; 167(3-4):600-8.VM

Abstract

This study presents results of quantitative pathogen detection by real-time PCR (qPCR) and phase-specific serology for complete Q fever diagnostics. For this, samples of 42 goats in total were taken during a Q fever outbreak. In the early phase of the Q-fever infection, 10(4)-10(8)Coxiella (C.) burnetii pathogens per vaginal swab and 10(2)-10(6)C. burnetii per ml milk were detected using quantitative real-time PCR (qPCR). Pathogen excretion decreased continuously within two months to less than 10(4) (vaginal swab) and 10(2) (milk) C. burnetii. At the end of the study there was a shift toward a 10 fold higher excretion of the pathogen via the genital tract and milk. At the start of the study, serological tests showed a dominance of the phase-2 antibody in 76% (22/29) of the goats in the MONA- (Multiple of Normal Activity) ELISA and 79% (23/29) in the IDEXX-ELISA, which was replaced by a phase-1 dominance in 85% (29/34) and 62% (21/34), of the animals respectively at the end of the study. Serum samples from 13 goats before lambing that excreted C. burnetii after lambing showed antibodies against phase 2 of 100% using MONA-ELISA and 77% in the IDEXX-ELISA. The most important diagnostic instrument for Q-fever infection in goats following birth is testing of vaginal swabs using qPCR. Phase-specific serology allows an estimation of possible pathogen excretion even before birth, as well as achieving valuable results for determination of the infection phase.

Authors+Show Affiliations

Chemisches und Veterinäruntersuchungsamt Stuttgart (Chemical and Veterinary Investigation Office Stuttgart), Schaflandstrasse 3/3, 70736 Fellbach, Germany. Electronic address: reinhard.sting@cvuas.bwl.de.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24095624

Citation

Sting, Reinhard, et al. "Quantitative Real-time PCR and Phase Specific Serology Are Mutually Supportive in Q Fever Diagnostics in Goats." Veterinary Microbiology, vol. 167, no. 3-4, 2013, pp. 600-8.
Sting R, Molz K, Philipp W, et al. Quantitative real-time PCR and phase specific serology are mutually supportive in Q fever diagnostics in goats. Vet Microbiol. 2013;167(3-4):600-8.
Sting, R., Molz, K., Philipp, W., Bothe, F., Runge, M., & Ganter, M. (2013). Quantitative real-time PCR and phase specific serology are mutually supportive in Q fever diagnostics in goats. Veterinary Microbiology, 167(3-4), 600-8. https://doi.org/10.1016/j.vetmic.2013.09.015
Sting R, et al. Quantitative Real-time PCR and Phase Specific Serology Are Mutually Supportive in Q Fever Diagnostics in Goats. Vet Microbiol. 2013 Dec 27;167(3-4):600-8. PubMed PMID: 24095624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative real-time PCR and phase specific serology are mutually supportive in Q fever diagnostics in goats. AU - Sting,Reinhard, AU - Molz,Kerstin, AU - Philipp,Werner, AU - Bothe,Friederike, AU - Runge,Martin, AU - Ganter,Martin, Y1 - 2013/09/18/ PY - 2013/08/08/received PY - 2013/09/07/revised PY - 2013/09/10/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/5/27/medline KW - Coxiella burnetii KW - Goats KW - Phase-specific ELISA KW - Q fever KW - Quantitative real-time PCR SP - 600 EP - 8 JF - Veterinary microbiology JO - Vet Microbiol VL - 167 IS - 3-4 N2 - This study presents results of quantitative pathogen detection by real-time PCR (qPCR) and phase-specific serology for complete Q fever diagnostics. For this, samples of 42 goats in total were taken during a Q fever outbreak. In the early phase of the Q-fever infection, 10(4)-10(8)Coxiella (C.) burnetii pathogens per vaginal swab and 10(2)-10(6)C. burnetii per ml milk were detected using quantitative real-time PCR (qPCR). Pathogen excretion decreased continuously within two months to less than 10(4) (vaginal swab) and 10(2) (milk) C. burnetii. At the end of the study there was a shift toward a 10 fold higher excretion of the pathogen via the genital tract and milk. At the start of the study, serological tests showed a dominance of the phase-2 antibody in 76% (22/29) of the goats in the MONA- (Multiple of Normal Activity) ELISA and 79% (23/29) in the IDEXX-ELISA, which was replaced by a phase-1 dominance in 85% (29/34) and 62% (21/34), of the animals respectively at the end of the study. Serum samples from 13 goats before lambing that excreted C. burnetii after lambing showed antibodies against phase 2 of 100% using MONA-ELISA and 77% in the IDEXX-ELISA. The most important diagnostic instrument for Q-fever infection in goats following birth is testing of vaginal swabs using qPCR. Phase-specific serology allows an estimation of possible pathogen excretion even before birth, as well as achieving valuable results for determination of the infection phase. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/24095624/Quantitative_real_time_PCR_and_phase_specific_serology_are_mutually_supportive_in_Q_fever_diagnostics_in_goats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(13)00454-9 DB - PRIME DP - Unbound Medicine ER -