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Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes.

Abstract

Silver nanoparticles (AgNPs) are present in a multitude of consumer and medical products; however, the toxicity of AgNPs is not fully understood. This research aimed to elucidate the relationship between AgNP cytotoxicity and oxidative stress and damage in rainbow trout (Oncorhynchus mykiss) hepatocytes and erythrocytes in comparison to silver ions (Ag(+)). Generally the cytotoxicity of AgNPs and Ag(+) was similar, such that both silver types generated reactive oxygen species, decreased glutathione levels, and decreased activities of glutathione reductase and glutathione-S-transferase. Nonetheless, the two silver types had different cellular targets; AgNPs increased lipid peroxidation without apparent uptake into the cells whereas Ag(+) increased DNA damage. Furthermore, the toxicity of both silver types was generally decreased in cells treated with cysteine while treatment with buthionine sulfoximine increased the toxicity of both silver types.

Authors+Show Affiliations

Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada. Electronic address: amass017@uottawa.ca.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24096131

Citation

Massarsky, Andrey, et al. "Nanosilver Cytotoxicity in Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes and Hepatocytes." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 159, 2014, pp. 10-21.
Massarsky A, Abraham R, Nguyen KC, et al. Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes. Comp Biochem Physiol C Toxicol Pharmacol. 2014;159:10-21.
Massarsky, A., Abraham, R., Nguyen, K. C., Rippstein, P., Tayabali, A. F., Trudeau, V. L., & Moon, T. W. (2014). Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 159, 10-21. https://doi.org/10.1016/j.cbpc.2013.09.008
Massarsky A, et al. Nanosilver Cytotoxicity in Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes and Hepatocytes. Comp Biochem Physiol C Toxicol Pharmacol. 2014;159:10-21. PubMed PMID: 24096131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes. AU - Massarsky,Andrey, AU - Abraham,Ren, AU - Nguyen,Kathy C, AU - Rippstein,Peter, AU - Tayabali,Azam F, AU - Trudeau,Vance L, AU - Moon,Thomas W, Y1 - 2013/10/03/ PY - 2013/06/24/received PY - 2013/09/25/revised PY - 2013/09/26/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/8/26/medline KW - Buthionine sulfoximine KW - Cysteine KW - Erythrocytes KW - Hepatocytes KW - Ionic silver toxicity KW - Nanomaterials KW - Nanosilver toxicity KW - Rainbow trout SP - 10 EP - 21 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 159 N2 - Silver nanoparticles (AgNPs) are present in a multitude of consumer and medical products; however, the toxicity of AgNPs is not fully understood. This research aimed to elucidate the relationship between AgNP cytotoxicity and oxidative stress and damage in rainbow trout (Oncorhynchus mykiss) hepatocytes and erythrocytes in comparison to silver ions (Ag(+)). Generally the cytotoxicity of AgNPs and Ag(+) was similar, such that both silver types generated reactive oxygen species, decreased glutathione levels, and decreased activities of glutathione reductase and glutathione-S-transferase. Nonetheless, the two silver types had different cellular targets; AgNPs increased lipid peroxidation without apparent uptake into the cells whereas Ag(+) increased DNA damage. Furthermore, the toxicity of both silver types was generally decreased in cells treated with cysteine while treatment with buthionine sulfoximine increased the toxicity of both silver types. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/24096131/Nanosilver_cytotoxicity_in_rainbow_trout__Oncorhynchus_mykiss__erythrocytes_and_hepatocytes_ DB - PRIME DP - Unbound Medicine ER -